In vivo recognition of an RNA aptamer by its transcription factor target

L. A. Cassiday, L James Maher III

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

In vitro-selected RNA aptamers are potential inhibitors of disease-relat ed macromolecules. Our laboratory previously isolated an RNA aptamer that specifically binds to the human transcription factor NF-κB. This RNA aptamer competitively inhibits DNA binding by NF-κB in vitro. In the study presented here, this aptamer was tested for binding to the p50 homodimer form of NF-κB (p502) in eukaryotic cells using a yeast three-hybrid system. We show that the α-p50 RNA aptamer selectively binds recombinant p502 expressed in yeast, demonstrating in vivo recognition of an in vitro-selected RNA aptamer by its protein target. This result suggests that RNA decoys might be used to inhibit the function of DNA-binding proteins in vivo.

Original languageEnglish (US)
Pages (from-to)2433-2438
Number of pages6
JournalBiochemistry
Volume40
Issue number8
DOIs
StatePublished - Feb 27 2001

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Nucleotide Aptamers
Transcription Factors
Yeast
Two-Hybrid System Techniques
DNA-Binding Proteins
Eukaryotic Cells
Hybrid systems
Macromolecules
Yeasts
RNA
DNA
In Vitro Techniques
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

In vivo recognition of an RNA aptamer by its transcription factor target. / Cassiday, L. A.; Maher III, L James.

In: Biochemistry, Vol. 40, No. 8, 27.02.2001, p. 2433-2438.

Research output: Contribution to journalArticle

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