In vivo oximetry using a nitroxide-liposome system

James Glockner, H. C. Chan, H. M. Swartz

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Liposomes containing the deuterated, charged, aqueous soluble nitroxide 4-trimethyl-ammonium-2,2,6,6-tetramethylpiperidine-d 16-1 -oxyl (d-Cat 1) were used as probes to measure oxygen concentrations in vivo. Following intramuscular or intraperitoneal injection of the liposome suspension, ICR mice were placed over the surface probe of a low frequency (1.1 GHz) electron paramagnetic resonance (EPR) spectrometer. The linewidth of the deuterated nitroxide is sensitive to changes in the dissolved oxygen concentration: this parameter was calibrated separately so that linewidths measured in the injected mice could be converted into oxygen tensions. This technique detected substantial changes in pO 2 as the oxygen content of the breathing gas was changed from 21 to 85 to 0%. Intravenous injection of the liposomes also is possible, and the liposomes accumulate in the liver and spleen, where detectable, oxygen-sensitive EPR signals can be measured.

Original languageEnglish (US)
Pages (from-to)123-133
Number of pages11
JournalMagnetic Resonance in Medicine
Volume20
Issue number1
StatePublished - 1991
Externally publishedYes

Fingerprint

Oximetry
Liposomes
Oxygen
Electron Spin Resonance Spectroscopy
Inbred ICR Mouse
Intramuscular Injections
Intraperitoneal Injections
Ammonium Compounds
Intravenous Injections
Suspensions
Respiration
Spleen
Gases
Liver

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

In vivo oximetry using a nitroxide-liposome system. / Glockner, James; Chan, H. C.; Swartz, H. M.

In: Magnetic Resonance in Medicine, Vol. 20, No. 1, 1991, p. 123-133.

Research output: Contribution to journalArticle

Glockner, J, Chan, HC & Swartz, HM 1991, 'In vivo oximetry using a nitroxide-liposome system', Magnetic Resonance in Medicine, vol. 20, no. 1, pp. 123-133.
Glockner, James ; Chan, H. C. ; Swartz, H. M. / In vivo oximetry using a nitroxide-liposome system. In: Magnetic Resonance in Medicine. 1991 ; Vol. 20, No. 1. pp. 123-133.
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