In vivo expression and function of hybrid Ia dimers (E(α)A(β)) in recombinant and transgenic mice

G. D. Anderson, C. S. David

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We have found cell surface expression of an E(α) molecule in recombinant and transgenic mouse strains lacking an E(β) molecule. Flow cytometry has shown low level expression of E(α) in B10.RQB3 (I-A(q)E(α)(k)) and B10.RFB2 (I-A(f)E(α)(k)) mice. We have also found that B10.Q (H-2(q)) mice can express the E(α)(k) transgene. Since these strains do not have functional E(β) chains, we propose that the E(α)A(β) hybrid dimers are formed in low numbers and can be picked up by FACS analysis. So far we have not been able to identify these hybrid molecules by cytotoxicity or immunoprecipitation. The E(α)/A(β) molecule can function in vivo during thymic selection in the clonal deletion of two V(β) TCR subsets, V(β)11 and V(β)6, which have been shown to interact with the intact I-E molecule.

Original languageEnglish (US)
Pages (from-to)1003-1008
Number of pages6
JournalJournal of Experimental Medicine
Volume170
Issue number3
StatePublished - 1989

Fingerprint

Transgenic Mice
Clonal Deletion
Transgenes
Immunoprecipitation
Flow Cytometry

ASJC Scopus subject areas

  • Immunology

Cite this

In vivo expression and function of hybrid Ia dimers (E(α)A(β)) in recombinant and transgenic mice. / Anderson, G. D.; David, C. S.

In: Journal of Experimental Medicine, Vol. 170, No. 3, 1989, p. 1003-1008.

Research output: Contribution to journalArticle

@article{be991f9e91cb4a1c970c9368716dab5d,
title = "In vivo expression and function of hybrid Ia dimers (E(α)A(β)) in recombinant and transgenic mice",
abstract = "We have found cell surface expression of an E(α) molecule in recombinant and transgenic mouse strains lacking an E(β) molecule. Flow cytometry has shown low level expression of E(α) in B10.RQB3 (I-A(q)E(α)(k)) and B10.RFB2 (I-A(f)E(α)(k)) mice. We have also found that B10.Q (H-2(q)) mice can express the E(α)(k) transgene. Since these strains do not have functional E(β) chains, we propose that the E(α)A(β) hybrid dimers are formed in low numbers and can be picked up by FACS analysis. So far we have not been able to identify these hybrid molecules by cytotoxicity or immunoprecipitation. The E(α)/A(β) molecule can function in vivo during thymic selection in the clonal deletion of two V(β) TCR subsets, V(β)11 and V(β)6, which have been shown to interact with the intact I-E molecule.",
author = "Anderson, {G. D.} and David, {C. S.}",
year = "1989",
language = "English (US)",
volume = "170",
pages = "1003--1008",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "3",

}

TY - JOUR

T1 - In vivo expression and function of hybrid Ia dimers (E(α)A(β)) in recombinant and transgenic mice

AU - Anderson, G. D.

AU - David, C. S.

PY - 1989

Y1 - 1989

N2 - We have found cell surface expression of an E(α) molecule in recombinant and transgenic mouse strains lacking an E(β) molecule. Flow cytometry has shown low level expression of E(α) in B10.RQB3 (I-A(q)E(α)(k)) and B10.RFB2 (I-A(f)E(α)(k)) mice. We have also found that B10.Q (H-2(q)) mice can express the E(α)(k) transgene. Since these strains do not have functional E(β) chains, we propose that the E(α)A(β) hybrid dimers are formed in low numbers and can be picked up by FACS analysis. So far we have not been able to identify these hybrid molecules by cytotoxicity or immunoprecipitation. The E(α)/A(β) molecule can function in vivo during thymic selection in the clonal deletion of two V(β) TCR subsets, V(β)11 and V(β)6, which have been shown to interact with the intact I-E molecule.

AB - We have found cell surface expression of an E(α) molecule in recombinant and transgenic mouse strains lacking an E(β) molecule. Flow cytometry has shown low level expression of E(α) in B10.RQB3 (I-A(q)E(α)(k)) and B10.RFB2 (I-A(f)E(α)(k)) mice. We have also found that B10.Q (H-2(q)) mice can express the E(α)(k) transgene. Since these strains do not have functional E(β) chains, we propose that the E(α)A(β) hybrid dimers are formed in low numbers and can be picked up by FACS analysis. So far we have not been able to identify these hybrid molecules by cytotoxicity or immunoprecipitation. The E(α)/A(β) molecule can function in vivo during thymic selection in the clonal deletion of two V(β) TCR subsets, V(β)11 and V(β)6, which have been shown to interact with the intact I-E molecule.

UR - http://www.scopus.com/inward/record.url?scp=0024457337&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024457337&partnerID=8YFLogxK

M3 - Article

C2 - 2788700

AN - SCOPUS:0024457337

VL - 170

SP - 1003

EP - 1008

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 3

ER -