TY - JOUR
T1 - In vivo dynamics of antigen-specific regulatory T cells not predicted from behavior in vitro
AU - Klein, Ludger
AU - Khazaie, Khashayarsha
AU - Von Boehmer, Harald
PY - 2003/7/22
Y1 - 2003/7/22
N2 - Adoptive transfer of antigen-specific CD25+CD4+ regulatory T cells was used to analyze the stability of their phenotype, their behavior after immunization, and their mode of suppressing cotransferred naive T cells in vivo. We found that regulatory T cells maintained their phenotype in the absence of antigen, were not anergic in vivo, and proliferated as extensively as naive CD4+ T cells after immunization without losing their suppressive function in vivo and in vitro. In vivo, the expansion of cotransferred naive T cells was suppressed relatively late in the response such that regulatory T cells expressing mostly IL-10 but not IL-2 or IFN-γ represented the dominant subset of cells. Our results reveal properties of regulatory T cells that were not predicted from in vitro studies.
AB - Adoptive transfer of antigen-specific CD25+CD4+ regulatory T cells was used to analyze the stability of their phenotype, their behavior after immunization, and their mode of suppressing cotransferred naive T cells in vivo. We found that regulatory T cells maintained their phenotype in the absence of antigen, were not anergic in vivo, and proliferated as extensively as naive CD4+ T cells after immunization without losing their suppressive function in vivo and in vitro. In vivo, the expansion of cotransferred naive T cells was suppressed relatively late in the response such that regulatory T cells expressing mostly IL-10 but not IL-2 or IFN-γ represented the dominant subset of cells. Our results reveal properties of regulatory T cells that were not predicted from in vitro studies.
UR - http://www.scopus.com/inward/record.url?scp=0041806547&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0041806547&partnerID=8YFLogxK
U2 - 10.1073/pnas.1533365100
DO - 10.1073/pnas.1533365100
M3 - Article
C2 - 12857946
AN - SCOPUS:0041806547
SN - 0027-8424
VL - 100
SP - 8886
EP - 8891
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 15
ER -