In vivo and in vitro studies of cytosolic phospholipase A2 expression in Helicobacter pylori infection

G. Nardone, E. L. Holicky, J. R. Uhl, L. Sabatino, S. Staibano, A. Rocco, V. Colantuoni, B. A. Manzo, M. Romano, G. Budillon, F. R. Cockerill, Laurence J Miller

Research output: Contribution to journalArticle

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Abstract

Modifications of mucosal phospholipids have been detected in samples from patients with Helicobacter pylori-positive gastritis. These alterations appear secondary to increased phospholipase A2 activity (PLA2). The cytosolic form of this enzyme (cPLA2), normally involved in cellular signaling and growth, has been implicated in cancer pathogenesis. The aim of this study was to investigate cPLA2 expression and PLA2 activity in the gastric mucosae of patients with and without H. pylori infection. In gastric biopsies from 10 H. pylori-positive patients, cPLA2 levels, levels of mRNA as determined by reverse transcriptase PCR, levels of protein as determined by immunohistochemistry, and total PLA2 activity were higher than in 10 H. pylori-negative gastritis patients. To clarify whether H. pylori had a direct effect on the cellular expression of cPLA2, we studied cPLA2 expression in vitro with different human epithelial cell lines, one from a patient with larynx carcinoma (i.e., HEp-2 cells) and two from patients with gastric adenocarcinoma (i.e., AGS and MKN 28 cells), incubated with different H. pylori strains. The levels of cPLA2, mRNA, and protein expression were unchanged in Hep-2 cells independently of cellular adhesion or invasion of the bacteria. Moreover, no change in cPLA2 protein expression was observed in AGS or MKN 28 cells treated with wild-type H. pylori. In conclusion, our study shows increased cPLA2 expression and PLA2 activity in the gastric mucosae of patients with H. pylori infection and no change in epithelial cell lines exposed to H. pylori.

Original languageEnglish (US)
Pages (from-to)5857-5863
Number of pages7
JournalInfection and Immunity
Volume69
Issue number9
DOIs
StatePublished - 2001
Externally publishedYes

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Cytosolic Phospholipases A2
Helicobacter Infections
Helicobacter pylori
Phospholipases A2
Gastritis
Gastric Mucosa
Stomach
Epithelial Cells
In Vitro Techniques
Cell Line
Messenger RNA
Proteins
Larynx
Reverse Transcriptase Polymerase Chain Reaction
Phospholipids
Adenocarcinoma
Immunohistochemistry
Bacteria
Carcinoma
Biopsy

ASJC Scopus subject areas

  • Immunology

Cite this

In vivo and in vitro studies of cytosolic phospholipase A2 expression in Helicobacter pylori infection. / Nardone, G.; Holicky, E. L.; Uhl, J. R.; Sabatino, L.; Staibano, S.; Rocco, A.; Colantuoni, V.; Manzo, B. A.; Romano, M.; Budillon, G.; Cockerill, F. R.; Miller, Laurence J.

In: Infection and Immunity, Vol. 69, No. 9, 2001, p. 5857-5863.

Research output: Contribution to journalArticle

Nardone, G, Holicky, EL, Uhl, JR, Sabatino, L, Staibano, S, Rocco, A, Colantuoni, V, Manzo, BA, Romano, M, Budillon, G, Cockerill, FR & Miller, LJ 2001, 'In vivo and in vitro studies of cytosolic phospholipase A2 expression in Helicobacter pylori infection', Infection and Immunity, vol. 69, no. 9, pp. 5857-5863. https://doi.org/10.1128/IAI.69.9.5857-5863.2001
Nardone, G. ; Holicky, E. L. ; Uhl, J. R. ; Sabatino, L. ; Staibano, S. ; Rocco, A. ; Colantuoni, V. ; Manzo, B. A. ; Romano, M. ; Budillon, G. ; Cockerill, F. R. ; Miller, Laurence J. / In vivo and in vitro studies of cytosolic phospholipase A2 expression in Helicobacter pylori infection. In: Infection and Immunity. 2001 ; Vol. 69, No. 9. pp. 5857-5863.
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AU - Holicky, E. L.

AU - Uhl, J. R.

AU - Sabatino, L.

AU - Staibano, S.

AU - Rocco, A.

AU - Colantuoni, V.

AU - Manzo, B. A.

AU - Romano, M.

AU - Budillon, G.

AU - Cockerill, F. R.

AU - Miller, Laurence J

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