TY - JOUR
T1 - In vivo 24-hydroxylation of 25-hydroxyvitamin D3 in enterocolectomized rats
AU - Kumar, Rajiv
AU - Silva, Patricio
AU - Epstein, Franklin H.
PY - 1979/6
Y1 - 1979/6
N2 - To determine if the renal 25-hydroxyvitamin D3 24-hydroxylase is active in addition to the intestinal 25-hydroxyvitamin D3 24-hydroxylase in vivo in the rat, radiolabeled 25-hydroxyvitamin D3 was administered to noftnocalcemic, vitamin D-replete, enterocolectomized rats. Substantial amounts of 24, 25-dihydroxyvitamin D3 were detected and the identity of the isolated plasma 24, 25-dihydroxyvitamin D3 region in these animals was confirmed by coelution on high pressure liquid chromatography and periodate cleavage. This suggests that both intestinal and renal 25-hydroxyvitamin D3 24-hydroxylases are active in vivo in the rat.
AB - To determine if the renal 25-hydroxyvitamin D3 24-hydroxylase is active in addition to the intestinal 25-hydroxyvitamin D3 24-hydroxylase in vivo in the rat, radiolabeled 25-hydroxyvitamin D3 was administered to noftnocalcemic, vitamin D-replete, enterocolectomized rats. Substantial amounts of 24, 25-dihydroxyvitamin D3 were detected and the identity of the isolated plasma 24, 25-dihydroxyvitamin D3 region in these animals was confirmed by coelution on high pressure liquid chromatography and periodate cleavage. This suggests that both intestinal and renal 25-hydroxyvitamin D3 24-hydroxylases are active in vivo in the rat.
UR - http://www.scopus.com/inward/record.url?scp=0018772263&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018772263&partnerID=8YFLogxK
U2 - 10.1210/endo-104-6-1794
DO - 10.1210/endo-104-6-1794
M3 - Article
C2 - 446398
AN - SCOPUS:0018772263
SN - 0013-7227
VL - 104
SP - 1794
EP - 1796
JO - Endocrinology
JF - Endocrinology
IS - 6
ER -