To determine if the renal 25-hydroxyvitamin D3 24-hydroxylase is active in addition to the intestinal 25-hydroxy-vitamin D3 24-hydroxylase in vivo in the rat, radiolabeled 25-hydroxyvitamin D3 was administered to normocalcemic, vitamin D-replete, enterocolectomized rats. Substantial amounts of 24,25-dihydroxyvitamin D3 were detected and the identity of the isolated plasma 24,25-dihydroxyvitamin D3 region in these animals was confirmed by coelution on high pressure liquid chromatography and periodate cleavage. This suggests that both intestinal and renal 25-hydroxyvitamin D3 24-hydroxylases are active in vivo in the rat.
|Original language||English (US)|
|Number of pages||3|
|State||Published - 1979|
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism