In vivo 24-hydroxylation of 25-hydroxyvitamin D3 in enterocolectomized rats

Rajiv Kumar, P. Silva, F. H. Epstein

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4 Scopus citations

Abstract

To determine if the renal 25-hydroxyvitamin D3 24-hydroxylase is active in addition to the intestinal 25-hydroxy-vitamin D3 24-hydroxylase in vivo in the rat, radiolabeled 25-hydroxyvitamin D3 was administered to normocalcemic, vitamin D-replete, enterocolectomized rats. Substantial amounts of 24,25-dihydroxyvitamin D3 were detected and the identity of the isolated plasma 24,25-dihydroxyvitamin D3 region in these animals was confirmed by coelution on high pressure liquid chromatography and periodate cleavage. This suggests that both intestinal and renal 25-hydroxyvitamin D3 24-hydroxylases are active in vivo in the rat.

Original languageEnglish (US)
Pages (from-to)1794-1796
Number of pages3
JournalEndocrinology
Volume104
Issue number6
StatePublished - 1979
Externally publishedYes

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ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

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