In vitro proliferation and adhesion of basic fibroblast growth factor- producing fibroblasts on platinum coils

David F Kallmes, M. Kathleen Borland, Harry J. Cloft, Talissa A. Altes, Jacques E. Dion, Mary E. Jensen, Gerald R. Hankins, Gregory A. Helm

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

PURPOSE: To evaluate the growth and adhesion characteristics in vitro of genetically modified basic fibroblast growth factor producing fibroblasts on platinum detachable coils. MATERIALS AND METHODS: Costs of two sizes were coated with laminin, poly L-lysine, fibronectin, and type I and type IV collagen and were cultured with basic fibroblast growth-factor secreting fibroblasts. Type I collagen strands were inserted in the lumen of some coils. Cellular proliferation and adherence during passage of coils through microcatheters were studied with both light and scanning electron microscopy. Growth factor concentration in the culture medium was measured. RESULTS: Rapid cellular proliferation was noted on all coated coils except those coated with type IV collagen. Proliferation on uncoated coils was slightly slower than on most coated coils, although confluent cell layers were present on uncoated larger-diameter coils within 48 hours. Cells had a marked propensity to grow between the primary coil. Windings into the coil lumen, except in coils that contained collagen filaments. Passage through microcatheters caused widespread stripping of cells from the outer surface of coils, especially the uncoated samples. Viable cells remained in the coil lumen. Supernatant contained high concentrations of growth. CONCLUSIONS: Platinum embolic coils are a promising mechanism of cell delivery for stimulation of scan formation or other desirable biologic effects.

Original languageEnglish (US)
Pages (from-to)237-243
Number of pages7
JournalRadiology
Volume206
Issue number1
StatePublished - Jan 1998
Externally publishedYes

Fingerprint

Fibroblast Growth Factor 2
Platinum
Fibroblasts
Collagen Type IV
Collagen Type I
Cell Proliferation
Laminin
Growth
Fibronectins
Electron Scanning Microscopy
Lysine
Culture Media
Intercellular Signaling Peptides and Proteins
Collagen
In Vitro Techniques
Light
Costs and Cost Analysis

Keywords

  • Aneurysm, cerebral
  • Cerebral blood vessels, therapeutic blockade

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

Kallmes, D. F., Borland, M. K., Cloft, H. J., Altes, T. A., Dion, J. E., Jensen, M. E., ... Helm, G. A. (1998). In vitro proliferation and adhesion of basic fibroblast growth factor- producing fibroblasts on platinum coils. Radiology, 206(1), 237-243.

In vitro proliferation and adhesion of basic fibroblast growth factor- producing fibroblasts on platinum coils. / Kallmes, David F; Borland, M. Kathleen; Cloft, Harry J.; Altes, Talissa A.; Dion, Jacques E.; Jensen, Mary E.; Hankins, Gerald R.; Helm, Gregory A.

In: Radiology, Vol. 206, No. 1, 01.1998, p. 237-243.

Research output: Contribution to journalArticle

Kallmes, DF, Borland, MK, Cloft, HJ, Altes, TA, Dion, JE, Jensen, ME, Hankins, GR & Helm, GA 1998, 'In vitro proliferation and adhesion of basic fibroblast growth factor- producing fibroblasts on platinum coils', Radiology, vol. 206, no. 1, pp. 237-243.
Kallmes DF, Borland MK, Cloft HJ, Altes TA, Dion JE, Jensen ME et al. In vitro proliferation and adhesion of basic fibroblast growth factor- producing fibroblasts on platinum coils. Radiology. 1998 Jan;206(1):237-243.
Kallmes, David F ; Borland, M. Kathleen ; Cloft, Harry J. ; Altes, Talissa A. ; Dion, Jacques E. ; Jensen, Mary E. ; Hankins, Gerald R. ; Helm, Gregory A. / In vitro proliferation and adhesion of basic fibroblast growth factor- producing fibroblasts on platinum coils. In: Radiology. 1998 ; Vol. 206, No. 1. pp. 237-243.
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AB - PURPOSE: To evaluate the growth and adhesion characteristics in vitro of genetically modified basic fibroblast growth factor producing fibroblasts on platinum detachable coils. MATERIALS AND METHODS: Costs of two sizes were coated with laminin, poly L-lysine, fibronectin, and type I and type IV collagen and were cultured with basic fibroblast growth-factor secreting fibroblasts. Type I collagen strands were inserted in the lumen of some coils. Cellular proliferation and adherence during passage of coils through microcatheters were studied with both light and scanning electron microscopy. Growth factor concentration in the culture medium was measured. RESULTS: Rapid cellular proliferation was noted on all coated coils except those coated with type IV collagen. Proliferation on uncoated coils was slightly slower than on most coated coils, although confluent cell layers were present on uncoated larger-diameter coils within 48 hours. Cells had a marked propensity to grow between the primary coil. Windings into the coil lumen, except in coils that contained collagen filaments. Passage through microcatheters caused widespread stripping of cells from the outer surface of coils, especially the uncoated samples. Viable cells remained in the coil lumen. Supernatant contained high concentrations of growth. CONCLUSIONS: Platinum embolic coils are a promising mechanism of cell delivery for stimulation of scan formation or other desirable biologic effects.

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