In vitro model of a fibrosa layer of a heart valve

Soumen Jana, Amir Lerman, Robert D. Simari

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The fibrosa layer of a cardiac aortic valve is composed mostly of a dense network of type I collagen fibers oriented in circumferential direction. This main layer bears the tensile load and responds to the high stress on a leaflet. The inner fibrosa layer is also the site of pathophysiologic changes that result in valvular dysfunction, including stenosis and regurgitation. In vitro studies of these changes are limited by the absence of a substrate that mimics the circumferentially oriented structure of the fibrosa layer. In heart valve tissue engineering, generation of this layer is challenging. This study aimed to develop an artificial fibrosa layer of a native aortic leaflet. A unique morphologically biomimicked, pliable, but standalone substrate with circumferentially oriented nanofibers was fabricated by electrospinning on a novel collector designed for this study. The substrate had low-bulk tensile stiffness and ultimate strength; thus, cultured valvular interstitial cells (VICs) showed a fibroblast phenotype that is generally observed in a healthy aortic leaflet. Furthermore, gene and protein expression and morphology of VICs in substrates were close to those in the fibrosa layer of a native aortic leaflet. This artificial fibrosa layer can be useful for in vitro studies of valvular dysfunctions.

Original languageEnglish (US)
Pages (from-to)20012-20020
Number of pages9
JournalACS Applied Materials and Interfaces
Volume7
Issue number36
DOIs
StatePublished - Sep 16 2015

Fingerprint

Substrates
Electrospinning
Fibroblasts
Nanofibers
Collagen Type I
Tissue engineering
Collagen
Loads (forces)
Genes
Stiffness
Proteins
Fibers
Direction compound

Keywords

  • cardiac valve
  • fibrosa layer
  • nanofibrous substrate
  • tissue engineering
  • valvular interstitial cells

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

In vitro model of a fibrosa layer of a heart valve. / Jana, Soumen; Lerman, Amir; Simari, Robert D.

In: ACS Applied Materials and Interfaces, Vol. 7, No. 36, 16.09.2015, p. 20012-20020.

Research output: Contribution to journalArticle

@article{dc17ffc5c29b45d794aee321c8ffe45f,
title = "In vitro model of a fibrosa layer of a heart valve",
abstract = "The fibrosa layer of a cardiac aortic valve is composed mostly of a dense network of type I collagen fibers oriented in circumferential direction. This main layer bears the tensile load and responds to the high stress on a leaflet. The inner fibrosa layer is also the site of pathophysiologic changes that result in valvular dysfunction, including stenosis and regurgitation. In vitro studies of these changes are limited by the absence of a substrate that mimics the circumferentially oriented structure of the fibrosa layer. In heart valve tissue engineering, generation of this layer is challenging. This study aimed to develop an artificial fibrosa layer of a native aortic leaflet. A unique morphologically biomimicked, pliable, but standalone substrate with circumferentially oriented nanofibers was fabricated by electrospinning on a novel collector designed for this study. The substrate had low-bulk tensile stiffness and ultimate strength; thus, cultured valvular interstitial cells (VICs) showed a fibroblast phenotype that is generally observed in a healthy aortic leaflet. Furthermore, gene and protein expression and morphology of VICs in substrates were close to those in the fibrosa layer of a native aortic leaflet. This artificial fibrosa layer can be useful for in vitro studies of valvular dysfunctions.",
keywords = "cardiac valve, fibrosa layer, nanofibrous substrate, tissue engineering, valvular interstitial cells",
author = "Soumen Jana and Amir Lerman and Simari, {Robert D.}",
year = "2015",
month = "9",
day = "16",
doi = "10.1021/acsami.5b04805",
language = "English (US)",
volume = "7",
pages = "20012--20020",
journal = "ACS applied materials & interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "36",

}

TY - JOUR

T1 - In vitro model of a fibrosa layer of a heart valve

AU - Jana, Soumen

AU - Lerman, Amir

AU - Simari, Robert D.

PY - 2015/9/16

Y1 - 2015/9/16

N2 - The fibrosa layer of a cardiac aortic valve is composed mostly of a dense network of type I collagen fibers oriented in circumferential direction. This main layer bears the tensile load and responds to the high stress on a leaflet. The inner fibrosa layer is also the site of pathophysiologic changes that result in valvular dysfunction, including stenosis and regurgitation. In vitro studies of these changes are limited by the absence of a substrate that mimics the circumferentially oriented structure of the fibrosa layer. In heart valve tissue engineering, generation of this layer is challenging. This study aimed to develop an artificial fibrosa layer of a native aortic leaflet. A unique morphologically biomimicked, pliable, but standalone substrate with circumferentially oriented nanofibers was fabricated by electrospinning on a novel collector designed for this study. The substrate had low-bulk tensile stiffness and ultimate strength; thus, cultured valvular interstitial cells (VICs) showed a fibroblast phenotype that is generally observed in a healthy aortic leaflet. Furthermore, gene and protein expression and morphology of VICs in substrates were close to those in the fibrosa layer of a native aortic leaflet. This artificial fibrosa layer can be useful for in vitro studies of valvular dysfunctions.

AB - The fibrosa layer of a cardiac aortic valve is composed mostly of a dense network of type I collagen fibers oriented in circumferential direction. This main layer bears the tensile load and responds to the high stress on a leaflet. The inner fibrosa layer is also the site of pathophysiologic changes that result in valvular dysfunction, including stenosis and regurgitation. In vitro studies of these changes are limited by the absence of a substrate that mimics the circumferentially oriented structure of the fibrosa layer. In heart valve tissue engineering, generation of this layer is challenging. This study aimed to develop an artificial fibrosa layer of a native aortic leaflet. A unique morphologically biomimicked, pliable, but standalone substrate with circumferentially oriented nanofibers was fabricated by electrospinning on a novel collector designed for this study. The substrate had low-bulk tensile stiffness and ultimate strength; thus, cultured valvular interstitial cells (VICs) showed a fibroblast phenotype that is generally observed in a healthy aortic leaflet. Furthermore, gene and protein expression and morphology of VICs in substrates were close to those in the fibrosa layer of a native aortic leaflet. This artificial fibrosa layer can be useful for in vitro studies of valvular dysfunctions.

KW - cardiac valve

KW - fibrosa layer

KW - nanofibrous substrate

KW - tissue engineering

KW - valvular interstitial cells

UR - http://www.scopus.com/inward/record.url?scp=84941760195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941760195&partnerID=8YFLogxK

U2 - 10.1021/acsami.5b04805

DO - 10.1021/acsami.5b04805

M3 - Article

C2 - 26295833

AN - SCOPUS:84941760195

VL - 7

SP - 20012

EP - 20020

JO - ACS applied materials & interfaces

JF - ACS applied materials & interfaces

SN - 1944-8244

IS - 36

ER -