TY - JOUR
T1 - In vitro gene transfer to chondrocytes and synovial fibroblasts by adenoviral vectors.
AU - Gouze, Jean Noel
AU - Stoddart, Martin J.
AU - Gouze, Elvire
AU - Palmer, Glyn D.
AU - Ghivizzani, Steven C.
AU - Grodzinsky, Alan J.
AU - Evans, Christopher H.
PY - 2004
Y1 - 2004
N2 - The major requirement of a successful gene transfer is the efficient delivery of an exogenous therapeutic gene to the appropriate cell type with subsequent high or regulated levels of expression. In this context, viral systems are more efficient than nonviral systems, giving higher levels of gene expression for longer periods. For the application of osteoarthritis (OA), gene products triggering anti-inflammatory or chondroprotective effects are of obvious therapeutic utility. Thus, their cognate genes are candidates for use in the gene therapy of OA. In this chapter, we describe the preparation, the use, and the effect of the transduction of chondrocytes or synovial fibroblasts with an adenoviral vector encoding the cDNA for glutamine: fructose-6-phosphate amidotransferase (GFAT). This is intended to serve as an example of a technology that can be used to evaluate the biological effects of overexpression of other cDNAs.
AB - The major requirement of a successful gene transfer is the efficient delivery of an exogenous therapeutic gene to the appropriate cell type with subsequent high or regulated levels of expression. In this context, viral systems are more efficient than nonviral systems, giving higher levels of gene expression for longer periods. For the application of osteoarthritis (OA), gene products triggering anti-inflammatory or chondroprotective effects are of obvious therapeutic utility. Thus, their cognate genes are candidates for use in the gene therapy of OA. In this chapter, we describe the preparation, the use, and the effect of the transduction of chondrocytes or synovial fibroblasts with an adenoviral vector encoding the cDNA for glutamine: fructose-6-phosphate amidotransferase (GFAT). This is intended to serve as an example of a technology that can be used to evaluate the biological effects of overexpression of other cDNAs.
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U2 - 10.1385/1-59259-810-2:147
DO - 10.1385/1-59259-810-2:147
M3 - Article
C2 - 15280594
AN - SCOPUS:16644397394
SN - 1543-1894
VL - 100
SP - 147
EP - 164
JO - Methods in molecular medicine
JF - Methods in molecular medicine
ER -