In vitro enhancing effects of thymic dendritic cells on apoptotic cell death of thymocytes

Haidong Dong, Jing Chen, Weifeng Chen

Research output: Contribution to journalArticle

Abstract

Using terminal deoxynucleotide transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) assay and propidium iodide-DNA staining flow cytometry assay, the effects of mouse thymic dendritic cells (MTSC4) on the process of programmed cell death of thymocytes in vitro were investigated. It was noticed that thymocytes bound to MTSC4 used in this study. That the percentages of apoptotic nuclei of the bound thymocytes on MTSC4 were much higher than those of medium-cultured thymocytes, while the bound thymocytes on mouse thymic epithelial cell (MTEC1) showed much lower percentages of apoptosis. FACS analysis quantitatively confirmed the observation. Phenotype analysis showed that MTSC4 induced the deletion of CD4 + CD8 + cells and CD4 + CD8- cell in 18 h of coculture. The results suggest that the negative selection of medullary thymocytes may be achieved by thymic dendritic cells through their enhancing effects on apoptosis.

Original languageEnglish (US)
Pages (from-to)87-92
Number of pages6
JournalScience in China, Series C: Life Sciences
Volume41
Issue number1
DOIs
StatePublished - Jan 1 1998

Keywords

  • Apoptosis
  • Thymic stromal cells
  • Thymocytes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

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