In vitro canine distemper virus infection of canine lymphoid cells

A prelude to oncolytic therapy for lymphoma

Steven E. Suter, May B. Chein, Veronika Von Messling, Becky Yip, Roberto Cattaneo, William Vernau, Bruce R. Madewell, Cheryl A. London

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Purpose: Measles virus (MV) causes the regression of human lymphoma xenografts. The purpose of this study was to determine if canine lymphoid cells could be infected in vitro with MV or canine distemper virus (CDV, the canine Morbillivirus equivalent of MV) and determine if in vitro viral infection leads to apoptotic cell death. Experimental Design: Reverse transcriptase-PCR was used to examine the expression of both signal lymphocyte activation molecule (CD150) and membrane cofactor molecule (CD46) mRNA. An attenuated CDV expressing enhanced green fluorescent protein was used to infect canine cells in vitro. Both flow cytometry and reverse transcriptase-PCR was used to document CDV infection. Cell death was examined using a propidium iodide staining assay and Annexin V binding. Results: Canine lymphoid cell lines and neoplastic B and T lymphocytes collected from dogs with spontaneous lymphoma expressed the Morbillivirus receptor CD150 mRNA. In contrast, only neoplastic lymphocytes expressed detectable levels of CD46 mRNA. Although MV did not infect canine cells, CDV efficiently infected between 40% and 70% of all three canine lymphoid lines tested. More importantly, CDV infected 50% to 90% of neoplastic lymphocytes isolated from dogs with both B and T cell lymphoma. Apoptosis of CDV-infected cell lines was documented. Conclusions: Attenuated CDV may be a useful treatment for canine lymphoma. As such, dogs with lymphoma may represent a biologically relevant large animal model to investigate the feasibility, safety, and efficacy of Morbillivirus therapy in a clinical setting with findings that may have direct applicability in the treatment of human non-Hodgkin's lymphoma.

Original languageEnglish (US)
Pages (from-to)1579-1587
Number of pages9
JournalClinical Cancer Research
Volume11
Issue number4
DOIs
StatePublished - Feb 15 2005

Fingerprint

Canine Distemper Virus
Virus Diseases
Canidae
Lymphoma
Lymphocytes
Measles virus
Morbillivirus
Dogs
Reverse Transcriptase Polymerase Chain Reaction
Messenger RNA
Therapeutics
Cell Death
Cell Line
Propidium
T-Cell Lymphoma
Annexin A5
B-Cell Lymphoma
Lymphocyte Activation
In Vitro Techniques
Heterografts

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

In vitro canine distemper virus infection of canine lymphoid cells : A prelude to oncolytic therapy for lymphoma. / Suter, Steven E.; Chein, May B.; Von Messling, Veronika; Yip, Becky; Cattaneo, Roberto; Vernau, William; Madewell, Bruce R.; London, Cheryl A.

In: Clinical Cancer Research, Vol. 11, No. 4, 15.02.2005, p. 1579-1587.

Research output: Contribution to journalArticle

Suter, Steven E. ; Chein, May B. ; Von Messling, Veronika ; Yip, Becky ; Cattaneo, Roberto ; Vernau, William ; Madewell, Bruce R. ; London, Cheryl A. / In vitro canine distemper virus infection of canine lymphoid cells : A prelude to oncolytic therapy for lymphoma. In: Clinical Cancer Research. 2005 ; Vol. 11, No. 4. pp. 1579-1587.
@article{3075b97e0ec54794b99ef3be94909b1a,
title = "In vitro canine distemper virus infection of canine lymphoid cells: A prelude to oncolytic therapy for lymphoma",
abstract = "Purpose: Measles virus (MV) causes the regression of human lymphoma xenografts. The purpose of this study was to determine if canine lymphoid cells could be infected in vitro with MV or canine distemper virus (CDV, the canine Morbillivirus equivalent of MV) and determine if in vitro viral infection leads to apoptotic cell death. Experimental Design: Reverse transcriptase-PCR was used to examine the expression of both signal lymphocyte activation molecule (CD150) and membrane cofactor molecule (CD46) mRNA. An attenuated CDV expressing enhanced green fluorescent protein was used to infect canine cells in vitro. Both flow cytometry and reverse transcriptase-PCR was used to document CDV infection. Cell death was examined using a propidium iodide staining assay and Annexin V binding. Results: Canine lymphoid cell lines and neoplastic B and T lymphocytes collected from dogs with spontaneous lymphoma expressed the Morbillivirus receptor CD150 mRNA. In contrast, only neoplastic lymphocytes expressed detectable levels of CD46 mRNA. Although MV did not infect canine cells, CDV efficiently infected between 40{\%} and 70{\%} of all three canine lymphoid lines tested. More importantly, CDV infected 50{\%} to 90{\%} of neoplastic lymphocytes isolated from dogs with both B and T cell lymphoma. Apoptosis of CDV-infected cell lines was documented. Conclusions: Attenuated CDV may be a useful treatment for canine lymphoma. As such, dogs with lymphoma may represent a biologically relevant large animal model to investigate the feasibility, safety, and efficacy of Morbillivirus therapy in a clinical setting with findings that may have direct applicability in the treatment of human non-Hodgkin's lymphoma.",
author = "Suter, {Steven E.} and Chein, {May B.} and {Von Messling}, Veronika and Becky Yip and Roberto Cattaneo and William Vernau and Madewell, {Bruce R.} and London, {Cheryl A.}",
year = "2005",
month = "2",
day = "15",
doi = "10.1158/1078-0432.CCR-04-1944",
language = "English (US)",
volume = "11",
pages = "1579--1587",
journal = "Clinical Cancer Research",
issn = "1078-0432",
publisher = "American Association for Cancer Research Inc.",
number = "4",

}

TY - JOUR

T1 - In vitro canine distemper virus infection of canine lymphoid cells

T2 - A prelude to oncolytic therapy for lymphoma

AU - Suter, Steven E.

AU - Chein, May B.

AU - Von Messling, Veronika

AU - Yip, Becky

AU - Cattaneo, Roberto

AU - Vernau, William

AU - Madewell, Bruce R.

AU - London, Cheryl A.

PY - 2005/2/15

Y1 - 2005/2/15

N2 - Purpose: Measles virus (MV) causes the regression of human lymphoma xenografts. The purpose of this study was to determine if canine lymphoid cells could be infected in vitro with MV or canine distemper virus (CDV, the canine Morbillivirus equivalent of MV) and determine if in vitro viral infection leads to apoptotic cell death. Experimental Design: Reverse transcriptase-PCR was used to examine the expression of both signal lymphocyte activation molecule (CD150) and membrane cofactor molecule (CD46) mRNA. An attenuated CDV expressing enhanced green fluorescent protein was used to infect canine cells in vitro. Both flow cytometry and reverse transcriptase-PCR was used to document CDV infection. Cell death was examined using a propidium iodide staining assay and Annexin V binding. Results: Canine lymphoid cell lines and neoplastic B and T lymphocytes collected from dogs with spontaneous lymphoma expressed the Morbillivirus receptor CD150 mRNA. In contrast, only neoplastic lymphocytes expressed detectable levels of CD46 mRNA. Although MV did not infect canine cells, CDV efficiently infected between 40% and 70% of all three canine lymphoid lines tested. More importantly, CDV infected 50% to 90% of neoplastic lymphocytes isolated from dogs with both B and T cell lymphoma. Apoptosis of CDV-infected cell lines was documented. Conclusions: Attenuated CDV may be a useful treatment for canine lymphoma. As such, dogs with lymphoma may represent a biologically relevant large animal model to investigate the feasibility, safety, and efficacy of Morbillivirus therapy in a clinical setting with findings that may have direct applicability in the treatment of human non-Hodgkin's lymphoma.

AB - Purpose: Measles virus (MV) causes the regression of human lymphoma xenografts. The purpose of this study was to determine if canine lymphoid cells could be infected in vitro with MV or canine distemper virus (CDV, the canine Morbillivirus equivalent of MV) and determine if in vitro viral infection leads to apoptotic cell death. Experimental Design: Reverse transcriptase-PCR was used to examine the expression of both signal lymphocyte activation molecule (CD150) and membrane cofactor molecule (CD46) mRNA. An attenuated CDV expressing enhanced green fluorescent protein was used to infect canine cells in vitro. Both flow cytometry and reverse transcriptase-PCR was used to document CDV infection. Cell death was examined using a propidium iodide staining assay and Annexin V binding. Results: Canine lymphoid cell lines and neoplastic B and T lymphocytes collected from dogs with spontaneous lymphoma expressed the Morbillivirus receptor CD150 mRNA. In contrast, only neoplastic lymphocytes expressed detectable levels of CD46 mRNA. Although MV did not infect canine cells, CDV efficiently infected between 40% and 70% of all three canine lymphoid lines tested. More importantly, CDV infected 50% to 90% of neoplastic lymphocytes isolated from dogs with both B and T cell lymphoma. Apoptosis of CDV-infected cell lines was documented. Conclusions: Attenuated CDV may be a useful treatment for canine lymphoma. As such, dogs with lymphoma may represent a biologically relevant large animal model to investigate the feasibility, safety, and efficacy of Morbillivirus therapy in a clinical setting with findings that may have direct applicability in the treatment of human non-Hodgkin's lymphoma.

UR - http://www.scopus.com/inward/record.url?scp=14644416551&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14644416551&partnerID=8YFLogxK

U2 - 10.1158/1078-0432.CCR-04-1944

DO - 10.1158/1078-0432.CCR-04-1944

M3 - Article

VL - 11

SP - 1579

EP - 1587

JO - Clinical Cancer Research

JF - Clinical Cancer Research

SN - 1078-0432

IS - 4

ER -