In vitro autoantibody production by normal adult and cord blood B cells

David S. Pisetsky, Diane F Jelinek, Laurie M. McAnally, Charles F. Reich, Peter E. Lipsky

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

To investigate the repertoire of autoantibodies in humans, anti-DNA and rheumatoid factor (RF) production in vitro was assessed in cultures of adult peripheral blood B cells and neonatal umbilical venous blood B cells. B cells were stimulated under various culture conditions, using an immobilized monoclonal anti-CD3 antibody and adult T cells or Staphylococcus aureus (SA) in the presence or absence of adult T cells or factors derived from mitogen-stimulated adult T cells as polyclonal B cell activators. Total IgM, as well as IgM anti-DNA and RF, were assessed by ELISA. Total IgM production was induced from adult and neonatal B cells with SA plus T cell factors, as well as anti-CD3-stimuIated T cells. RF was induced from adult and cord blood B cells by either mode of stimulation, whereas significant anti-DNA production was observed only when B cells were stimulated with anti-CD3-activated T cells. These results confirm the presence of B cell precursors for autoantibodies in the preimmune as well as normal adult repertoire, and indicate that the production of anti-DNA and RF appears to be regulated independently.

Original languageEnglish (US)
Pages (from-to)899-903
Number of pages5
JournalJournal of Clinical Investigation
Volume85
Issue number3
StatePublished - Mar 1990
Externally publishedYes

Fingerprint

Fetal Blood
Autoantibodies
Blood Cells
B-Lymphocytes
Rheumatoid Factor
T-Lymphocytes
DNA
Immunoglobulin M
Staphylococcus aureus
TCF Transcription Factors
Umbilicus
B-Lymphoid Precursor Cells
Mitogens
In Vitro Techniques
Anti-Idiotypic Antibodies
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies

Keywords

  • Anti-CD3, B cell stimulation
  • Anti-DNA
  • B cell repertoire
  • Neonatal immune response
  • Rheumatoid factor

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Pisetsky, D. S., Jelinek, D. F., McAnally, L. M., Reich, C. F., & Lipsky, P. E. (1990). In vitro autoantibody production by normal adult and cord blood B cells. Journal of Clinical Investigation, 85(3), 899-903.

In vitro autoantibody production by normal adult and cord blood B cells. / Pisetsky, David S.; Jelinek, Diane F; McAnally, Laurie M.; Reich, Charles F.; Lipsky, Peter E.

In: Journal of Clinical Investigation, Vol. 85, No. 3, 03.1990, p. 899-903.

Research output: Contribution to journalArticle

Pisetsky, DS, Jelinek, DF, McAnally, LM, Reich, CF & Lipsky, PE 1990, 'In vitro autoantibody production by normal adult and cord blood B cells', Journal of Clinical Investigation, vol. 85, no. 3, pp. 899-903.
Pisetsky, David S. ; Jelinek, Diane F ; McAnally, Laurie M. ; Reich, Charles F. ; Lipsky, Peter E. / In vitro autoantibody production by normal adult and cord blood B cells. In: Journal of Clinical Investigation. 1990 ; Vol. 85, No. 3. pp. 899-903.
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