In vitro autoantibody production by normal adult and cord blood B cells

David S. Pisetsky, Diane F. Jelinek, Laurie M. McAnally, Charles F. Reich, Peter E. Lipsky

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

To investigate the repertoire of autoantibodies in humans, anti-DNA and rheumatoid factor (RF) production in vitro was assessed in cultures of adult peripheral blood B cells and neonatal umbilical venous blood B cells. B cells were stimulated under various culture conditions, using an immobilized monoclonal anti-CD3 antibody and adult T cells or Staphylococcus aureus (SA) in the presence or absence of adult T cells or factors derived from mitogen-stimulated adult T cells as polyclonal B cell activators. Total IgM, as well as IgM anti-DNA and RF, were assessed by ELISA. Total IgM production was induced from adult and neonatal B cells with SA plus T cell factors, as well as anti-CD3-stimuIated T cells. RF was induced from adult and cord blood B cells by either mode of stimulation, whereas significant anti-DNA production was observed only when B cells were stimulated with anti-CD3-activated T cells. These results confirm the presence of B cell precursors for autoantibodies in the preimmune as well as normal adult repertoire, and indicate that the production of anti-DNA and RF appears to be regulated independently.

Original languageEnglish (US)
Pages (from-to)899-903
Number of pages5
JournalJournal of Clinical Investigation
Volume85
Issue number3
DOIs
StatePublished - Mar 1990

Keywords

  • Anti-CD3, B cell stimulation
  • Anti-DNA
  • B cell repertoire
  • Neonatal immune response
  • Rheumatoid factor

ASJC Scopus subject areas

  • Medicine(all)

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