In situ nuclear organization of regulatory machinery

Shirwin M. Pockwinse, Sayyed K. Zaidi, Ricardo F. Medina, Rachit Bakshi, Krishna P. Kota, Syed A. Ali, Daniel W. Young, Jeffery A. Nickerson, Amjad Javed, Martin Montecino, Andre J. Van Wijnen, Jane B. Lian, Janet L. Stein, Gary S. Stein

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages239-259
Number of pages21
Volume455
DOIs
StatePublished - 2008
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume455
ISSN (Print)10643745

Keywords

  • Cell synchrony
  • Confocal microscopy
  • FRAP
  • Immunofluorescence microscopy
  • Live cell microscopy
  • Nuclear matrix
  • Nuclear organization
  • Runx

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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