In situ nuclear organization of regulatory machinery

Shirwin M. Pockwinse; Sayyed K. Zaidi; Ricardo F. Medina; Rachit Bakshi; Krishna P. Kota; Syed A. Ali; Daniel W. Young; Jeffery A. Nickerson; Amjad Javed; Martin Montecino; Andre J. Van Wijnen; Jane B. Lian; Janet L. Stein; Gary S. Stein

doi:10.1007/978-1-59745-104-8_17

In situ nuclear organization of regulatory machinery

Shirwin M. Pockwinse, Sayyed K. Zaidi, Ricardo F. Medina, Rachit Bakshi, Krishna P. Kota, Syed A. Ali, Daniel W. Young, Jeffery A. Nickerson, Amjad Javed, Martin Montecino, Andre J. Van Wijnen, Jane B. Lian, Janet L. Stein, Gary S. Stein

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Abstract

Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

Original language	English (US)
Title of host publication	Osteoporosis
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press
Pages	239-259
Number of pages	21
ISBN (Print)	9781588298287
DOIs	https://doi.org/10.1007/978-1-59745-104-8_17
State	Published - 2008

Publication series

Name	Methods in Molecular Biology
Volume	455
ISSN (Print)	1064-3745

Keywords

Cell synchrony
Confocal microscopy
FRAP
Immunofluorescence microscopy
Live cell microscopy
Nuclear matrix
Nuclear organization
Runx

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-59745-104-8_17

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Pockwinse, S. M., Zaidi, S. K., Medina, R. F., Bakshi, R., Kota, K. P., Ali, S. A., Young, D. W., Nickerson, J. A., Javed, A., Montecino, M., Van Wijnen, A. J., Lian, J. B., Stein, J. L., & Stein, G. S. (2008). In situ nuclear organization of regulatory machinery. In Osteoporosis: Methods and Protocols (pp. 239-259). (Methods in Molecular Biology; Vol. 455). Humana Press. https://doi.org/10.1007/978-1-59745-104-8_17

Pockwinse, SM, Zaidi, SK, Medina, RF, Bakshi, R, Kota, KP, Ali, SA, Young, DW, Nickerson, JA, Javed, A, Montecino, M, Van Wijnen, AJ, Lian, JB, Stein, JL & Stein, GS 2008, In situ nuclear organization of regulatory machinery. in Osteoporosis: Methods and Protocols. Methods in Molecular Biology, vol. 455, Humana Press, pp. 239-259. https://doi.org/10.1007/978-1-59745-104-8_17

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abstract = "Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.",

keywords = "Cell synchrony, Confocal microscopy, FRAP, Immunofluorescence microscopy, Live cell microscopy, Nuclear matrix, Nuclear organization, Runx",

author = "Pockwinse, {Shirwin M.} and Zaidi, {Sayyed K.} and Medina, {Ricardo F.} and Rachit Bakshi and Kota, {Krishna P.} and Ali, {Syed A.} and Young, {Daniel W.} and Nickerson, {Jeffery A.} and Amjad Javed and Martin Montecino and {Van Wijnen}, {Andre J.} and Lian, {Jane B.} and Stein, {Janet L.} and Stein, {Gary S.}",

year = "2008",

doi = "10.1007/978-1-59745-104-8_17",

language = "English (US)",

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series = "Methods in Molecular Biology",

publisher = "Humana Press",

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AU - Pockwinse, Shirwin M.

AU - Zaidi, Sayyed K.

AU - Medina, Ricardo F.

AU - Bakshi, Rachit

AU - Kota, Krishna P.

AU - Ali, Syed A.

AU - Young, Daniel W.

AU - Nickerson, Jeffery A.

AU - Javed, Amjad

AU - Montecino, Martin

AU - Van Wijnen, Andre J.

AU - Lian, Jane B.

AU - Stein, Janet L.

AU - Stein, Gary S.

PY - 2008

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AB - Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

KW - Cell synchrony

KW - Confocal microscopy

KW - FRAP

KW - Immunofluorescence microscopy

KW - Live cell microscopy

KW - Nuclear matrix

KW - Nuclear organization

KW - Runx

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ER -

In situ nuclear organization of regulatory machinery

Abstract

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Keywords

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