In situ hybridization in paracoccidioidomycosis

Thales De Brito; G. S. Sandhu; B. C. Kline; R. A. Aleff; M. P. Sandoval; R. T. Santos; A. A.H. Brandão; C. S. Lacaz

doi:10.1046/j.1365-280X.1999.00222.x

In situ hybridization in paracoccidioidomycosis

Thales De Brito, G. S. Sandhu, B. C. Kline, R. A. Aleff, M. P. Sandoval, R. T. Santos, A. A.H. Brandão, C. S. Lacaz

Cardiovascular Medicine

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence. When combining ISH with the Gridley stain which detects fungal cell walls, about 2-3% of the fungal cells present in the tissues were labelled. When the complementary probe was used, labelling was higher, reaching the 3% level.

Original language	English (US)
Pages (from-to)	207-211
Number of pages	5
Journal	Medical mycology
Volume	37
Issue number	3
DOIs	https://doi.org/10.1046/j.1365-280X.1999.00222.x
State	Published - Jun 1999

Keywords

Human paracoccidioidomycosis
In situ hybridization
Oral biopsy
Paracoccidioides brasiliensis

ASJC Scopus subject areas

Infectious Diseases

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10.1046/j.1365-280X.1999.00222.x

Cite this

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title = "In situ hybridization in paracoccidioidomycosis",

abstract = "In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence. When combining ISH with the Gridley stain which detects fungal cell walls, about 2-3% of the fungal cells present in the tissues were labelled. When the complementary probe was used, labelling was higher, reaching the 3% level.",

keywords = "Human paracoccidioidomycosis, In situ hybridization, Oral biopsy, Paracoccidioides brasiliensis",

author = "{De Brito}, Thales and Sandhu, {G. S.} and Kline, {B. C.} and Aleff, {R. A.} and Sandoval, {M. P.} and Santos, {R. T.} and Brand{\~a}o, {A. A.H.} and Lacaz, {C. S.}",

note = "Funding Information: Financial support for this project was provided by CNPq (300857:94-2) and LIM 06 and 53.",

year = "1999",

month = jun,

doi = "10.1046/j.1365-280X.1999.00222.x",

language = "English (US)",

volume = "37",

pages = "207--211",

journal = "Medical mycology",

issn = "1369-3786",

publisher = "Informa Healthcare",

number = "3",

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T1 - In situ hybridization in paracoccidioidomycosis

AU - De Brito, Thales

AU - Sandhu, G. S.

AU - Kline, B. C.

AU - Aleff, R. A.

AU - Sandoval, M. P.

AU - Santos, R. T.

AU - Brandão, A. A.H.

AU - Lacaz, C. S.

N1 - Funding Information: Financial support for this project was provided by CNPq (300857:94-2) and LIM 06 and 53.

PY - 1999/6

Y1 - 1999/6

N2 - In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence. When combining ISH with the Gridley stain which detects fungal cell walls, about 2-3% of the fungal cells present in the tissues were labelled. When the complementary probe was used, labelling was higher, reaching the 3% level.

AB - In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence. When combining ISH with the Gridley stain which detects fungal cell walls, about 2-3% of the fungal cells present in the tissues were labelled. When the complementary probe was used, labelling was higher, reaching the 3% level.

KW - Human paracoccidioidomycosis

KW - In situ hybridization

KW - Oral biopsy

KW - Paracoccidioides brasiliensis

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DO - 10.1046/j.1365-280X.1999.00222.x

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JO - Medical mycology

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In situ hybridization in paracoccidioidomycosis

Abstract

Keywords

ASJC Scopus subject areas

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