In situ hybridization in paracoccidioidomycosis

Thales De Brito, G. S. Sandhu, B. C. Kline, R. A. Aleff, M. P. Sandoval, R. T. Santos, A. A.H. Brandão, C. S. Lacaz

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence. When combining ISH with the Gridley stain which detects fungal cell walls, about 2-3% of the fungal cells present in the tissues were labelled. When the complementary probe was used, labelling was higher, reaching the 3% level.

Original languageEnglish (US)
Pages (from-to)207-211
Number of pages5
JournalMedical mycology
Volume37
Issue number3
DOIs
StatePublished - Jun 1 1999

Keywords

  • Human paracoccidioidomycosis
  • In situ hybridization
  • Oral biopsy
  • Paracoccidioides brasiliensis

ASJC Scopus subject areas

  • Infectious Diseases

Fingerprint Dive into the research topics of 'In situ hybridization in paracoccidioidomycosis'. Together they form a unique fingerprint.

  • Cite this

    De Brito, T., Sandhu, G. S., Kline, B. C., Aleff, R. A., Sandoval, M. P., Santos, R. T., Brandão, A. A. H., & Lacaz, C. S. (1999). In situ hybridization in paracoccidioidomycosis. Medical mycology, 37(3), 207-211. https://doi.org/10.1046/j.1365-280X.1999.00222.x