In situ hybridization in paracoccidioidomycosis

Thales De Brito, G. S. Sandhu, B. C. Kline, R. A. Aleff, M. P. Sandoval, R. T. Santos, A. A H Brandão, C. S. Lacaz

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence. When combining ISH with the Gridley stain which detects fungal cell walls, about 2-3% of the fungal cells present in the tissues were labelled. When the complementary probe was used, labelling was higher, reaching the 3% level.

Original languageEnglish (US)
Pages (from-to)207-211
Number of pages5
JournalMedical Mycology
Volume37
Issue number3
DOIs
StatePublished - Jun 1999

Fingerprint

Paracoccidioidomycosis
in situ hybridization
In Situ Hybridization
Paracoccidioides
Paracoccidioides brasiliensis
Base Pairing
guinea pigs
Cell Wall
Testis
biopsy
mouth
testes
Guinea Pigs
Coloring Agents
Soil
cell walls
Biopsy
soil
cells
tissues

Keywords

  • Human paracoccidioidomycosis
  • In situ hybridization
  • Oral biopsy
  • Paracoccidioides brasiliensis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • veterinary(all)
  • Microbiology

Cite this

De Brito, T., Sandhu, G. S., Kline, B. C., Aleff, R. A., Sandoval, M. P., Santos, R. T., ... Lacaz, C. S. (1999). In situ hybridization in paracoccidioidomycosis. Medical Mycology, 37(3), 207-211. https://doi.org/10.1046/j.1365-280X.1999.00222.x

In situ hybridization in paracoccidioidomycosis. / De Brito, Thales; Sandhu, G. S.; Kline, B. C.; Aleff, R. A.; Sandoval, M. P.; Santos, R. T.; Brandão, A. A H; Lacaz, C. S.

In: Medical Mycology, Vol. 37, No. 3, 06.1999, p. 207-211.

Research output: Contribution to journalArticle

De Brito, T, Sandhu, GS, Kline, BC, Aleff, RA, Sandoval, MP, Santos, RT, Brandão, AAH & Lacaz, CS 1999, 'In situ hybridization in paracoccidioidomycosis', Medical Mycology, vol. 37, no. 3, pp. 207-211. https://doi.org/10.1046/j.1365-280X.1999.00222.x
De Brito T, Sandhu GS, Kline BC, Aleff RA, Sandoval MP, Santos RT et al. In situ hybridization in paracoccidioidomycosis. Medical Mycology. 1999 Jun;37(3):207-211. https://doi.org/10.1046/j.1365-280X.1999.00222.x
De Brito, Thales ; Sandhu, G. S. ; Kline, B. C. ; Aleff, R. A. ; Sandoval, M. P. ; Santos, R. T. ; Brandão, A. A H ; Lacaz, C. S. / In situ hybridization in paracoccidioidomycosis. In: Medical Mycology. 1999 ; Vol. 37, No. 3. pp. 207-211.
@article{956395b6876d4f1fb99c8885a3990005,
title = "In situ hybridization in paracoccidioidomycosis",
abstract = "In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence. When combining ISH with the Gridley stain which detects fungal cell walls, about 2-3{\%} of the fungal cells present in the tissues were labelled. When the complementary probe was used, labelling was higher, reaching the 3{\%} level.",
keywords = "Human paracoccidioidomycosis, In situ hybridization, Oral biopsy, Paracoccidioides brasiliensis",
author = "{De Brito}, Thales and Sandhu, {G. S.} and Kline, {B. C.} and Aleff, {R. A.} and Sandoval, {M. P.} and Santos, {R. T.} and Brand{\~a}o, {A. A H} and Lacaz, {C. S.}",
year = "1999",
month = "6",
doi = "10.1046/j.1365-280X.1999.00222.x",
language = "English (US)",
volume = "37",
pages = "207--211",
journal = "Medical Mycology",
issn = "1369-3786",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - In situ hybridization in paracoccidioidomycosis

AU - De Brito, Thales

AU - Sandhu, G. S.

AU - Kline, B. C.

AU - Aleff, R. A.

AU - Sandoval, M. P.

AU - Santos, R. T.

AU - Brandão, A. A H

AU - Lacaz, C. S.

PY - 1999/6

Y1 - 1999/6

N2 - In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence. When combining ISH with the Gridley stain which detects fungal cell walls, about 2-3% of the fungal cells present in the tissues were labelled. When the complementary probe was used, labelling was higher, reaching the 3% level.

AB - In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence. When combining ISH with the Gridley stain which detects fungal cell walls, about 2-3% of the fungal cells present in the tissues were labelled. When the complementary probe was used, labelling was higher, reaching the 3% level.

KW - Human paracoccidioidomycosis

KW - In situ hybridization

KW - Oral biopsy

KW - Paracoccidioides brasiliensis

UR - http://www.scopus.com/inward/record.url?scp=0032787810&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032787810&partnerID=8YFLogxK

U2 - 10.1046/j.1365-280X.1999.00222.x

DO - 10.1046/j.1365-280X.1999.00222.x

M3 - Article

VL - 37

SP - 207

EP - 211

JO - Medical Mycology

JF - Medical Mycology

SN - 1369-3786

IS - 3

ER -