TY - JOUR
T1 - In humans at least 75% of insulin secretion arises from punctuated insulin secretory bursts
AU - Pørksen, Niels
AU - Nyholm, Birgit
AU - Veldhuis, Johannes D.
AU - Butler, Peter C.
AU - Schmitz, Ole
PY - 1997
Y1 - 1997
N2 - Detection of insulin secretory bursts in peripheral blood is hampered by hepatic insulin extraction, dilution in the systemic insulin pool, and time- delayed damping of secretory burst amplitude. Previous studies in dogs in vive and other experiments in vitro have shown that ~70% of all insulin is released within distinct insulin secretory bursts. To establish a method for detection and quantification of pulsatile insulin release in humans on the basis of peripheral insulin concentration measurements, we used a high- sensitivity, -specificity, and -precision insulin enzyme-linked immunosorbent assay (ELISA) and optimized an established deconvolution methodology to quantify the frequency, mass, and amplitude of insulin secretory bursts as well as to estimate the relative contribution of pulsatile insulin release to overall insulin secretion. By use of minutely sampled serum insulin concentrations measured by a highly sensitive insulin ELISA, and insulin kinetics of 2.8 min (first half-life), 5.0 min (second half-life), and a fractional slow component of 0.28, the deconvolved insulin secretion rates in 20 healthy subjects during glucose infusion (4.5 mg · kg-1 · min-1) could be resolved into a series (4.7 ± 0.1 min/pulse) of approximately symmetric insulin secretory bursts with a mean mass of 87 ± 12 pmol · l- 1 · pulse-1 and a mean amplitude (maximal release rate) of 35 ± 4.7 pmol · l-1 · min-1. The relative contribution of pulsatile to overall insulin secretion was 75 ± 1.6% (range 59-85%). We conclude that in vive insulin secretion in humans during nominal glucose stimulation consists of a series of punctuated insulin secretory bursts accounting for ≤75% of total insulin secretion.
AB - Detection of insulin secretory bursts in peripheral blood is hampered by hepatic insulin extraction, dilution in the systemic insulin pool, and time- delayed damping of secretory burst amplitude. Previous studies in dogs in vive and other experiments in vitro have shown that ~70% of all insulin is released within distinct insulin secretory bursts. To establish a method for detection and quantification of pulsatile insulin release in humans on the basis of peripheral insulin concentration measurements, we used a high- sensitivity, -specificity, and -precision insulin enzyme-linked immunosorbent assay (ELISA) and optimized an established deconvolution methodology to quantify the frequency, mass, and amplitude of insulin secretory bursts as well as to estimate the relative contribution of pulsatile insulin release to overall insulin secretion. By use of minutely sampled serum insulin concentrations measured by a highly sensitive insulin ELISA, and insulin kinetics of 2.8 min (first half-life), 5.0 min (second half-life), and a fractional slow component of 0.28, the deconvolved insulin secretion rates in 20 healthy subjects during glucose infusion (4.5 mg · kg-1 · min-1) could be resolved into a series (4.7 ± 0.1 min/pulse) of approximately symmetric insulin secretory bursts with a mean mass of 87 ± 12 pmol · l- 1 · pulse-1 and a mean amplitude (maximal release rate) of 35 ± 4.7 pmol · l-1 · min-1. The relative contribution of pulsatile to overall insulin secretion was 75 ± 1.6% (range 59-85%). We conclude that in vive insulin secretion in humans during nominal glucose stimulation consists of a series of punctuated insulin secretory bursts accounting for ≤75% of total insulin secretion.
KW - Amplitude
KW - C-peptide
KW - Glucose
KW - Oscillations
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U2 - 10.1152/ajpendo.1997.273.5.e908
DO - 10.1152/ajpendo.1997.273.5.e908
M3 - Article
C2 - 9374676
AN - SCOPUS:0030658922
SN - 0193-1849
VL - 273
SP - E908-E914
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
IS - 5 36-5
ER -