Improved tendon healing using bFGF, BMP-12 and TGFβ1 in a rat model

M. Majewski, P. Heisterbach, C. Jaquiéry, L. Dürselen, A. Todorov, I. Martin, Christopher H Evans, Sebastian A. Müller

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Several growth factors (GFs) are expressed as tendons heal, but it remains unknown whether their combined application enhances the healing process. This matter was addressed by applying a combination of basic fibroblast growth factor (bFGF), bone morphogenetic protein 12 (BMP-12) and transforming growth factor beta 1 (TGFβ1) in a rat Achilles tendon transection model. GFs were applied in one of the three following ways: i) direct application of all three factors at the time of surgery; ii) sequential, tiered percutaneous injection of individual factors immediately after surgery, 48 h and 96 h later; iii) load of all three factors onto a collagen sponge implanted at the time of surgery. After 1, 2, 4 and 8 weeks, healing was assessed based on tendon length and thickness, mechanical strength, stiffness and histology. Best results were achieved when GFs were loaded onto a collagen sponge – with a rapid increase in mechanical strength (load to failure, 71.2 N vs. 7.7 N in controls), consistent tendon length over time (9.9 mm vs. 16.2 mm in controls) and faster tendon remodelling, as measured by histology – followed by tiered injection therapy over 96 h. In conclusion, implantation of a GF-loaded collagen sponge could provide a promising treatment, especially in high-performance athletes and revision cases prone to re-rupture. For conservative treatment, tiered percutaneous GF application could be an option for improving clinical outcome.

Original languageEnglish (US)
Pages (from-to)318-334
Number of pages17
JournalEuropean Cells and Materials
Volume35
DOIs
StatePublished - Jan 1 2018

Fingerprint

Tendons
Fibroblast Growth Factor 2
Fibroblasts
Transforming Growth Factor beta
Rats
Intercellular Signaling Peptides and Proteins
Bone
Proteins
Porifera
Surgery
Histology
Collagen
Strength of materials
Injections
Achilles Tendon
Athletes
Rupture
Loads (forces)
Stiffness
growth differentiation factor 7

Keywords

  • Achilles tendon
  • Growth factors
  • Rat
  • Tendon engineering
  • Tendon healing

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering
  • Cell Biology

Cite this

Majewski, M., Heisterbach, P., Jaquiéry, C., Dürselen, L., Todorov, A., Martin, I., ... Müller, S. A. (2018). Improved tendon healing using bFGF, BMP-12 and TGFβ1 in a rat model. European Cells and Materials, 35, 318-334. https://doi.org/10.22203/eCM.v035a22

Improved tendon healing using bFGF, BMP-12 and TGFβ1 in a rat model. / Majewski, M.; Heisterbach, P.; Jaquiéry, C.; Dürselen, L.; Todorov, A.; Martin, I.; Evans, Christopher H; Müller, Sebastian A.

In: European Cells and Materials, Vol. 35, 01.01.2018, p. 318-334.

Research output: Contribution to journalArticle

Majewski, M, Heisterbach, P, Jaquiéry, C, Dürselen, L, Todorov, A, Martin, I, Evans, CH & Müller, SA 2018, 'Improved tendon healing using bFGF, BMP-12 and TGFβ1 in a rat model', European Cells and Materials, vol. 35, pp. 318-334. https://doi.org/10.22203/eCM.v035a22
Majewski M, Heisterbach P, Jaquiéry C, Dürselen L, Todorov A, Martin I et al. Improved tendon healing using bFGF, BMP-12 and TGFβ1 in a rat model. European Cells and Materials. 2018 Jan 1;35:318-334. https://doi.org/10.22203/eCM.v035a22
Majewski, M. ; Heisterbach, P. ; Jaquiéry, C. ; Dürselen, L. ; Todorov, A. ; Martin, I. ; Evans, Christopher H ; Müller, Sebastian A. / Improved tendon healing using bFGF, BMP-12 and TGFβ1 in a rat model. In: European Cells and Materials. 2018 ; Vol. 35. pp. 318-334.
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