Improved staining method for determining the extent of thermal damage to cells

Margaret E. Sherwood, Thomas J Flotte

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background and Objective: Enzyme histochemical stains of frozen sections have been used by investigators to assess thermal damage. The assessment of thermal damage to cells in lipid-rich tissues such as subcutaneous tissue and sebaceous glands can be difficult due to the quality of frozen sections of such tissues. The purpose of this study is to develop an improved method for this type of evaluation. Study Design/Materials and Methods: Thick frozen sections of thermally damaged pig and human skin were stained for lactate dehydrogenase. The sections were fixed in formalin and processed for paraffin-embedded sections. Results: The sections showed well-defined localization of the enzymatic deposits as well as preservation of the tissue architecture. Conclusion: The paraffin-embedded lactate dehydrogenase stained sections provide improved evaluation of thermally damaged tissues, particularly the lipid rich tissues.

Original languageEnglish (US)
Pages (from-to)128-131
Number of pages4
JournalLasers in Surgery and Medicine
Volume39
Issue number2
DOIs
StatePublished - Feb 2007
Externally publishedYes

Fingerprint

Frozen Sections
Hot Temperature
Staining and Labeling
L-Lactate Dehydrogenase
Paraffin
Tissue Preservation
Lipids
Sebaceous Glands
Subcutaneous Tissue
Formaldehyde
Coloring Agents
Swine
Research Personnel
Skin
Enzymes

Keywords

  • Lactate dehydrogenase
  • Pathology
  • Thermal damage

ASJC Scopus subject areas

  • Surgery

Cite this

Improved staining method for determining the extent of thermal damage to cells. / Sherwood, Margaret E.; Flotte, Thomas J.

In: Lasers in Surgery and Medicine, Vol. 39, No. 2, 02.2007, p. 128-131.

Research output: Contribution to journalArticle

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