Improved enzymatic hydrolysis of conjugated bile acids

W. Edward Highsmith; Paul M. Hyde

doi:10.1007/BF02534877

Improved enzymatic hydrolysis of conjugated bile acids

W. Edward Highsmith, Paul M. Hyde

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

Abstract

The hydrolysis of conjugated bile acids by cholylglycine hydrolase (EC 3.5.1.24) using the standard procedure for a commercial enzyme preparation was found to be incomplete, as judged by the use of 24-¹⁴C-taurocholic acid as a tracer. A method is proposed that incorporates the nonionic detergent Triton X-100 into the reaction mixture to achieve almost complete hydrolysis. It is proposed that the observed enhancement of enzyme activity is due to the formation of micelles by the detergent.

Original language	English (US)
Pages (from-to)	61-63
Number of pages	3
Journal	Lipids
Volume	22
Issue number	1
DOIs	https://doi.org/10.1007/BF02534877
State	Published - Jan 1 1987

ASJC Scopus subject areas

Biochemistry
Organic Chemistry
Cell Biology

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abstract = "The hydrolysis of conjugated bile acids by cholylglycine hydrolase (EC 3.5.1.24) using the standard procedure for a commercial enzyme preparation was found to be incomplete, as judged by the use of 24-14C-taurocholic acid as a tracer. A method is proposed that incorporates the nonionic detergent Triton X-100 into the reaction mixture to achieve almost complete hydrolysis. It is proposed that the observed enhancement of enzyme activity is due to the formation of micelles by the detergent.",

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