Improved enzymatic hydrolysis of conjugated bile acids

W. Edward Highsmith, Paul M. Hyde

Research output: Contribution to journalArticlepeer-review

Abstract

The hydrolysis of conjugated bile acids by cholylglycine hydrolase (EC 3.5.1.24) using the standard procedure for a commercial enzyme preparation was found to be incomplete, as judged by the use of 24-14C-taurocholic acid as a tracer. A method is proposed that incorporates the nonionic detergent Triton X-100 into the reaction mixture to achieve almost complete hydrolysis. It is proposed that the observed enhancement of enzyme activity is due to the formation of micelles by the detergent.

Original languageEnglish (US)
Pages (from-to)61-63
Number of pages3
JournalLipids
Volume22
Issue number1
DOIs
StatePublished - Jan 1 1987

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

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