Importance of the vasculature in cyst formation after spinal cord injury: Laboratory investigation

Gemma E. Rooney, Toshiki Endo, Syed Ameenuddin, Bingkun Chen, Sandeep Vaishya, LouAnn Gross, Terry K. Schiefer, Bradford L. Currier, Robert J. Spinner, Michael J Yaszemski, Anthony John Windebank

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Object. Glial scar and cystic formation greatly contribute to the inhibition of axonal regeneration after spinal cord injury (SCI). Attempts to promote axonal regeneration are extremely challenging in this type of hostile environment. The objective of this study was to examine the surgical methods that may be used to assess the factors that influence the level of scar and cystic formation in SCI. Methods. In the first part of this study, a complete transection was performed at vertebral level T9-10 in adult female Sprague-Dawley rats. The dura mater was either left open (control group) or was closed using sutures or hyaluronic acid. In the second part of the study, complete or subpial transection was performed, with the same dural closure technique applied to both groups. Histological analysis of longitudinal sections of the spinal cord was performed, and the percentage of scar and cyst formation was determined. Results. Dural closure using sutures resulted in significantly less glial scar formation (p = 0.0248), while incorporation of the subpial transection surgical technique was then shown to significantly decrease cyst formation (p < 0.0001). Conclusions. In this study, the authors demonstrated the importance of the vasculature in cyst formation after spinal cord trauma and confirmed the importance of dural closure in reducing glial scar formation.

Original languageEnglish (US)
Pages (from-to)432-437
Number of pages6
JournalJournal of Neurosurgery: Spine
Volume11
Issue number4
DOIs
StatePublished - Oct 2009

Fingerprint

Spinal Cord Injuries
Cicatrix
Cysts
Neuroglia
Sutures
Regeneration
Dura Mater
Hyaluronic Acid
Sprague Dawley Rats
Spinal Cord
Control Groups

Keywords

  • Glial cell response to injury
  • Traumatic spinal cord injury
  • Vascular injury

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery
  • Neurology

Cite this

Importance of the vasculature in cyst formation after spinal cord injury : Laboratory investigation. / Rooney, Gemma E.; Endo, Toshiki; Ameenuddin, Syed; Chen, Bingkun; Vaishya, Sandeep; Gross, LouAnn; Schiefer, Terry K.; Currier, Bradford L.; Spinner, Robert J.; Yaszemski, Michael J; Windebank, Anthony John.

In: Journal of Neurosurgery: Spine, Vol. 11, No. 4, 10.2009, p. 432-437.

Research output: Contribution to journalArticle

Rooney, GE, Endo, T, Ameenuddin, S, Chen, B, Vaishya, S, Gross, L, Schiefer, TK, Currier, BL, Spinner, RJ, Yaszemski, MJ & Windebank, AJ 2009, 'Importance of the vasculature in cyst formation after spinal cord injury: Laboratory investigation', Journal of Neurosurgery: Spine, vol. 11, no. 4, pp. 432-437. https://doi.org/10.3171/2009.4.SPINE08784
Rooney, Gemma E. ; Endo, Toshiki ; Ameenuddin, Syed ; Chen, Bingkun ; Vaishya, Sandeep ; Gross, LouAnn ; Schiefer, Terry K. ; Currier, Bradford L. ; Spinner, Robert J. ; Yaszemski, Michael J ; Windebank, Anthony John. / Importance of the vasculature in cyst formation after spinal cord injury : Laboratory investigation. In: Journal of Neurosurgery: Spine. 2009 ; Vol. 11, No. 4. pp. 432-437.
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