Implications of heterogeneous bead behavior on cell mechanical properties measured with magnetic twisting cytometry

Ben Fabry; Geoffrey N. Maksym; Rolf D. Hubmayr; James P. Butler; Jeffrey J. Fredberg

doi:10.1016/S0304-8853(98)00564-2

Implications of heterogeneous bead behavior on cell mechanical properties measured with magnetic twisting cytometry

Ben Fabry, Geoffrey N. Maksym, Rolf D. Hubmayr, James P. Butler, Jeffrey J. Fredberg

Pulmonary and Critical Care Medicine

Research output: Contribution to journal › Conference article › peer-review

72 Scopus citations

Abstract

Magnetic twisting cytometry (MTC) measures cellular mechanical properties, such as cell stiffness and viscosity, by applying mechanical stress to specific cell surface receptors via ligand-coated ferromagnetic beads. MTC measures simultaneously the rotation of approximately 50,000 beads attached to 20,000-40,000 cells. Here we show direct evidence of heterogeneous bead behavior and examine its consequences in the interpretation of cell mechanical properties.

Original language	English (US)
Pages (from-to)	120-125
Number of pages	6
Journal	Journal of Magnetism and Magnetic Materials
Volume	194
Issue number	1
DOIs	https://doi.org/10.1016/S0304-8853(98)00564-2
State	Published - Apr 1999
Event	Proceedings of 1998 2nd International Conference on Scientific and Clinical Applications of Magnetic Carriers - Cleveland, OH, USA Duration: May 28 1998 → May 30 1998

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics

Access to Document

10.1016/S0304-8853(98)00564-2

Cite this

@article{8ce0c6cbc6ac4a5d9de8965f572b4044,

title = "Implications of heterogeneous bead behavior on cell mechanical properties measured with magnetic twisting cytometry",

abstract = "Magnetic twisting cytometry (MTC) measures cellular mechanical properties, such as cell stiffness and viscosity, by applying mechanical stress to specific cell surface receptors via ligand-coated ferromagnetic beads. MTC measures simultaneously the rotation of approximately 50,000 beads attached to 20,000-40,000 cells. Here we show direct evidence of heterogeneous bead behavior and examine its consequences in the interpretation of cell mechanical properties.",

author = "Ben Fabry and Maksym, {Geoffrey N.} and Hubmayr, {Rolf D.} and Butler, {James P.} and Fredberg, {Jeffrey J.}",

note = "Funding Information: We thank Tori Hatch for her invaluable help with scanning EM. Ning Wang has supplied the BCE cells and materials for MTC and helped us mastering the MTC technique. Rey Panettieri (University of Pennsylvania) has supplied the HASM cells. We thank In Lim, Johanne Laporte, Paul Moore and Jianxin Chen for technical assistance with the cell preparation. This work was supported by the Swiss National Foundation, Deutsche Forschungsgemeinschaft, NIH and NSERC Canada.; Proceedings of 1998 2nd International Conference on Scientific and Clinical Applications of Magnetic Carriers ; Conference date: 28-05-1998 Through 30-05-1998",

year = "1999",

month = apr,

doi = "10.1016/S0304-8853(98)00564-2",

language = "English (US)",

volume = "194",

pages = "120--125",

journal = "Journal of Magnetism and Magnetic Materials",

issn = "0304-8853",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Implications of heterogeneous bead behavior on cell mechanical properties measured with magnetic twisting cytometry

AU - Fabry, Ben

AU - Maksym, Geoffrey N.

AU - Hubmayr, Rolf D.

AU - Butler, James P.

AU - Fredberg, Jeffrey J.

N1 - Funding Information: We thank Tori Hatch for her invaluable help with scanning EM. Ning Wang has supplied the BCE cells and materials for MTC and helped us mastering the MTC technique. Rey Panettieri (University of Pennsylvania) has supplied the HASM cells. We thank In Lim, Johanne Laporte, Paul Moore and Jianxin Chen for technical assistance with the cell preparation. This work was supported by the Swiss National Foundation, Deutsche Forschungsgemeinschaft, NIH and NSERC Canada.

PY - 1999/4

Y1 - 1999/4

N2 - Magnetic twisting cytometry (MTC) measures cellular mechanical properties, such as cell stiffness and viscosity, by applying mechanical stress to specific cell surface receptors via ligand-coated ferromagnetic beads. MTC measures simultaneously the rotation of approximately 50,000 beads attached to 20,000-40,000 cells. Here we show direct evidence of heterogeneous bead behavior and examine its consequences in the interpretation of cell mechanical properties.

AB - Magnetic twisting cytometry (MTC) measures cellular mechanical properties, such as cell stiffness and viscosity, by applying mechanical stress to specific cell surface receptors via ligand-coated ferromagnetic beads. MTC measures simultaneously the rotation of approximately 50,000 beads attached to 20,000-40,000 cells. Here we show direct evidence of heterogeneous bead behavior and examine its consequences in the interpretation of cell mechanical properties.

UR - http://www.scopus.com/inward/record.url?scp=0032633379&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032633379&partnerID=8YFLogxK

U2 - 10.1016/S0304-8853(98)00564-2

DO - 10.1016/S0304-8853(98)00564-2

M3 - Conference article

AN - SCOPUS:0032633379

SN - 0304-8853

VL - 194

SP - 120

EP - 125

JO - Journal of Magnetism and Magnetic Materials

JF - Journal of Magnetism and Magnetic Materials

IS - 1

T2 - Proceedings of 1998 2nd International Conference on Scientific and Clinical Applications of Magnetic Carriers

Y2 - 28 May 1998 through 30 May 1998

ER -

Implications of heterogeneous bead behavior on cell mechanical properties measured with magnetic twisting cytometry

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this