Implementing heterogeneous immunoassay using channel and droplets in digital microfluidic platform

Y. Liu, I. Papautsky

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this work, we demonstrate a heterogeneous immunoassay in digital microfluidic platform that integrates continuous flow channel and discrete droplet functionalities. A typical digital microfluidics-based heterogeneous immunoassay requires multiple cycles of droplet-based dilution for sample wash-ing, and costly detection equipment such as photomultiplier tube [1]. Here we adopt both channel and droplets in the immunoassay protocol, permitting the use of nL of sample volume and an effective washing approach that leads to 10 pg/mL of limit-of-detection with simple colorimetric image analysis.

Original languageEnglish (US)
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages894-895
Number of pages2
ISBN (Electronic)9780979806490
StatePublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: Oct 9 2016Oct 13 2016

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period10/9/1610/13/16

Keywords

  • Digital microfluidics
  • Elisa
  • Heterogeneous immunoassay
  • Magnetic beads

ASJC Scopus subject areas

  • Control and Systems Engineering

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