TY - GEN
T1 - Implementing heterogeneous immunoassay using channel and droplets in digital microfluidic platform
AU - Liu, Y.
AU - Papautsky, I.
N1 - Funding Information:
We gratefully acknowledge support by the National Science Foundation (ECCS-1001141) and Ohio Center for Microfluidic Innovation at University of Cincinnati.
PY - 2016
Y1 - 2016
N2 - In this work, we demonstrate a heterogeneous immunoassay in digital microfluidic platform that integrates continuous flow channel and discrete droplet functionalities. A typical digital microfluidics-based heterogeneous immunoassay requires multiple cycles of droplet-based dilution for sample wash-ing, and costly detection equipment such as photomultiplier tube [1]. Here we adopt both channel and droplets in the immunoassay protocol, permitting the use of nL of sample volume and an effective washing approach that leads to 10 pg/mL of limit-of-detection with simple colorimetric image analysis.
AB - In this work, we demonstrate a heterogeneous immunoassay in digital microfluidic platform that integrates continuous flow channel and discrete droplet functionalities. A typical digital microfluidics-based heterogeneous immunoassay requires multiple cycles of droplet-based dilution for sample wash-ing, and costly detection equipment such as photomultiplier tube [1]. Here we adopt both channel and droplets in the immunoassay protocol, permitting the use of nL of sample volume and an effective washing approach that leads to 10 pg/mL of limit-of-detection with simple colorimetric image analysis.
KW - Digital microfluidics
KW - Elisa
KW - Heterogeneous immunoassay
KW - Magnetic beads
UR - http://www.scopus.com/inward/record.url?scp=85014228230&partnerID=8YFLogxK
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M3 - Conference contribution
AN - SCOPUS:85014228230
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 894
EP - 895
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Y2 - 9 October 2016 through 13 October 2016
ER -