Impaired Ca2+-dependent activation of large-conductance Ca 2+-activated K+ channels in the coronary artery smooth muscle cells of Zucker diabetic fatty rats

Tong D Lu, Dan Ye, Tongrong He, Xiao Li Wang, Hai Long Wang, Hon Chi Lee

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Abstract

The large-conductance Ca2+-activated K+ (BK) channels play an important role in the regulation of cellular excitability in response to changes in intracellular metabolic state and Ca2+ homeostasis. In vascular smooth muscle, BK channels are key determinants of vasoreactivity and vital-organ perfusion. Vascular BK channel functions are impaired in diabetes mellitus, but the mechanisms underlying such changes have not been examined in detail. We examined and compared the activities and kinetics of BK channels in coronary arterial smooth muscle cells from Lean control and Zucker Diabetic Fatty (ZDF) rats, using single-channel recording techniques. We found that BK channels in ZDF rats have impaired Ca2+ sensitivity, including an increased free Ca2+ concentration at half-maximal effect on channel activation, a reduced steepness of Ca2+ dose-dependent curve, altered Ca2+-dependent gating properties with decreased maximal open probability, and a shortened mean open-time and prolonged mean closed-time durations. In addition, the BK channel β-subunit-mediated activation by dehydrosoyasaponin-1 (DHS-1) was lost in cells from ZDF rats. Immunoblotting analysis confirmed a 2.1-fold decrease in BK channel β1-subunit expression in ZDF rats, compared with that of Lean rats. These abnormalities in BK channel gating lead to an increase in the energy barrier for channel activation, and may contribute to the development of vascular dysfunction and complications in type 2 diabetes mellitus.

Original languageEnglish (US)
Pages (from-to)5165-5177
Number of pages13
JournalBiophysical Journal
Volume95
Issue number11
DOIs
StatePublished - Dec 1 2008

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Large-Conductance Calcium-Activated Potassium Channels
Smooth Muscle Myocytes
Coronary Vessels
Blood Vessels
Calcium-Activated Potassium Channels
Vascular Smooth Muscle
Immunoblotting
Type 2 Diabetes Mellitus
Diabetes Mellitus
Homeostasis
Perfusion

ASJC Scopus subject areas

  • Biophysics

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Impaired Ca2+-dependent activation of large-conductance Ca 2+-activated K+ channels in the coronary artery smooth muscle cells of Zucker diabetic fatty rats. / Lu, Tong D; Ye, Dan; He, Tongrong; Wang, Xiao Li; Wang, Hai Long; Lee, Hon Chi.

In: Biophysical Journal, Vol. 95, No. 11, 01.12.2008, p. 5165-5177.

Research output: Contribution to journalArticle

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