Impact of TIEG1 deletion on the passive mechanical properties of fast and slow twitch skeletal muscles in female mice

Malek Kammoun, Philippe Pouletaut, Francis Canon, Malayannan Subramaniam, John R Hawse, Muriel Vayssade, Sabine F. Bensamoun

Research output: Contribution to journalArticle

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Abstract

As transforming growth factor (TGF)-β inducible early gene-1 is highly expressed in skeletal muscle, the effect of TIEG1 gene deletion on the passive mechanical properties of slow and fast twitch muscle fibers was analyzed. Twenty five muscle fibers were harvested from soleus (Sol) and extensor digitorum longus (EDL) muscles from TIEG1-/- (N = 5) and control (N = 5) mice. Mechanical tests were performed on fibers and the dynamic and static stresses were measured. A viscoelastic Hill model of 3rd order was used to fit the experimental relaxation test data. In parallel, immunohistochemical analyses were performed on three serial transverse sections to detect the myosin isoforms within the slow and fast muscles. The percentage and the mean cross sectional area of each fiber type were calculated. These tests revealed a significant increase in the mechanical stress properties for the TIEG1-/- Sol fibers while a significant decrease appeared for the TIEG1-/- EDL fibers. Hill model tracked the shape of the experimental relaxation curve for both genotypes and both fiber types. Immunohistochemical results showed hypertrophy of all fiber types for TIEG1 TIEG1-/- muscles with an increase in the percentage of glycolytic fibers (IIX, and IIB) and a decrease of oxidative fibers (I, and IIA). This study has provided new insights into the role of TIEG1, known as KLF10, in the functional (SoltypeI: more resistant, EDLtypeIIB: less resistant) and morphological (glycolytic hypertrophy) properties of fast and slow twitch skeletal muscles. Further investigation at the cellular level will better reveal the role of the TIEG1 gene in skeletal muscle tissue.

Original languageEnglish (US)
Article numbere0164566
JournalPLoS One
Volume11
Issue number10
DOIs
StatePublished - Oct 1 2016

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mechanical properties
Muscle
skeletal muscle
Skeletal Muscle
hypertrophy
muscle fibers
Muscles
Mechanical properties
muscles
Fibers
mice
transforming growth factors
mechanical stress
gene deletion
Hypertrophy
testing
myosin
muscle tissues
Slow-Twitch Muscle Fibers
Fast-Twitch Muscle Fibers

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Impact of TIEG1 deletion on the passive mechanical properties of fast and slow twitch skeletal muscles in female mice. / Kammoun, Malek; Pouletaut, Philippe; Canon, Francis; Subramaniam, Malayannan; Hawse, John R; Vayssade, Muriel; Bensamoun, Sabine F.

In: PLoS One, Vol. 11, No. 10, e0164566, 01.10.2016.

Research output: Contribution to journalArticle

Kammoun, Malek ; Pouletaut, Philippe ; Canon, Francis ; Subramaniam, Malayannan ; Hawse, John R ; Vayssade, Muriel ; Bensamoun, Sabine F. / Impact of TIEG1 deletion on the passive mechanical properties of fast and slow twitch skeletal muscles in female mice. In: PLoS One. 2016 ; Vol. 11, No. 10.
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