Impact of RNA degradation on fusion detection by RNA-seq

Jaime I. Davila, Numrah M. Fadra, Xiaoke Wang, Amber M. McDonald, Asha A. Nair, Barbara R. Crusan, Xianglin Wu, Joseph H. Blommel, Jin Jen, Kandelaria M. Rumilla, Robert Brian Jenkins, Umut Aypar, Eric W Klee, Benjamin R. Kipp, Kevin C. Halling

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: RNA-seq is a well-established method for studying the transcriptome. Popular methods for library preparation in RNA-seq such as Illumina TruSeq® RNA v2 kit use a poly-A pulldown strategy. Such methods can cause loss of coverage at the 5' end of genes, impacting the ability to detect fusions when used on degraded samples. The goal of this study was to quantify the effects RNA degradation has on fusion detection when using poly-A selected mRNA and to identify the variables involved in this process. Results: Using both artificially and naturally degraded samples, we found that there is a reduced ability to detect fusions as the distance of the breakpoint from the 3' end of the gene increases. The median transcript coverage decreases exponentially as a function of the distance from the 3' end and there is a linear relationship between the coverage decay rate and the RNA integrity number (RIN). Based on these findings we developed plots that show the probability of detecting a gene fusion ("sensitivity") as a function of the distance of the fusion breakpoint from the 3' end. Conclusions: This study developed a strategy to assess the impact that RNA degradation has on the ability to detect gene fusions by RNA-seq.

Original languageEnglish (US)
Article number814
JournalBMC Genomics
Volume17
Issue number1
DOIs
StatePublished - Oct 20 2016

Fingerprint

RNA Stability
RNA
Gene Fusion
Poly A
Transcriptome
Genes
Libraries
Messenger RNA

Keywords

  • Fusion detection
  • Poly-A pulldown
  • RNA degradation
  • RNA-seq

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

Davila, J. I., Fadra, N. M., Wang, X., McDonald, A. M., Nair, A. A., Crusan, B. R., ... Halling, K. C. (2016). Impact of RNA degradation on fusion detection by RNA-seq. BMC Genomics, 17(1), [814]. https://doi.org/10.1186/s12864-016-3161-9

Impact of RNA degradation on fusion detection by RNA-seq. / Davila, Jaime I.; Fadra, Numrah M.; Wang, Xiaoke; McDonald, Amber M.; Nair, Asha A.; Crusan, Barbara R.; Wu, Xianglin; Blommel, Joseph H.; Jen, Jin; Rumilla, Kandelaria M.; Jenkins, Robert Brian; Aypar, Umut; Klee, Eric W; Kipp, Benjamin R.; Halling, Kevin C.

In: BMC Genomics, Vol. 17, No. 1, 814, 20.10.2016.

Research output: Contribution to journalArticle

Davila, JI, Fadra, NM, Wang, X, McDonald, AM, Nair, AA, Crusan, BR, Wu, X, Blommel, JH, Jen, J, Rumilla, KM, Jenkins, RB, Aypar, U, Klee, EW, Kipp, BR & Halling, KC 2016, 'Impact of RNA degradation on fusion detection by RNA-seq', BMC Genomics, vol. 17, no. 1, 814. https://doi.org/10.1186/s12864-016-3161-9
Davila JI, Fadra NM, Wang X, McDonald AM, Nair AA, Crusan BR et al. Impact of RNA degradation on fusion detection by RNA-seq. BMC Genomics. 2016 Oct 20;17(1). 814. https://doi.org/10.1186/s12864-016-3161-9
Davila, Jaime I. ; Fadra, Numrah M. ; Wang, Xiaoke ; McDonald, Amber M. ; Nair, Asha A. ; Crusan, Barbara R. ; Wu, Xianglin ; Blommel, Joseph H. ; Jen, Jin ; Rumilla, Kandelaria M. ; Jenkins, Robert Brian ; Aypar, Umut ; Klee, Eric W ; Kipp, Benjamin R. ; Halling, Kevin C. / Impact of RNA degradation on fusion detection by RNA-seq. In: BMC Genomics. 2016 ; Vol. 17, No. 1.
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