Impact of hepatic arterial reconstruction on orthotopic liver transplantation in the rat

Tomohide Hori, Lindsay B. Gardner, Florence Chen, Ann Marie T. Baine, Toshiyuki Hata, Shinji Uemoto, Justin H. Nguyen

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Orthotopic liver transplantation (OLT) models in rats have been investigated in many studies, but detailed information on the impact of hepatic artery (HA) reconstruction on postoperative factors remains to be investigated. HA reconstruction also requires advanced skills. The effect of the reconstruction of the HA by a hand-suture technique in rats with a whole-liver syngeneic graft was investigated. Long-term survival, histopathological assessment, immunohistological evaluation, and blood biochemistry were investigated until postoperative day (POD) 28. From the early postoperative period, significant differences between OLTs with or without HA reconstruction were found in graft parenchymal damage, induction of apoptosis, and transaminase levels, though survival curves and the coagulation profile showed no differences. In OLT without HA reconstruction, biliary proliferation was decreased at POD 5-14, and total bilirubin level was increased at PODs 10 and 14. The study indicates that HA reconstruction is required for reliable OLT in rats.

Original languageEnglish (US)
Pages (from-to)242-252
Number of pages11
JournalJournal of Investigative Surgery
Volume25
Issue number4
DOIs
StatePublished - Aug 1 2012

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Keywords

  • Hepatic artery
  • Microsurgery

ASJC Scopus subject areas

  • Surgery

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