Impact of Epithelial-Stromal Interactions on Peritumoral Fibroblasts in Ductal Carcinoma in Situ

Carina Strell, Janna Paulsson, Shao Bo Jin, Nicholas P. Tobin, Artur Mezheyeuski, Pernilla Roswall, Ceren Mutgan, Nicholas Mitsios, Hemming Johansson, Sarah Marie Wickberg, Jessica Svedlund, Mats Nilsson, Per Hall, Jan Mulder, Derek C. Radisky, Kristian Pietras, Jonas Bergh, Urban Lendahl, Fredrik Wärnberg, Arne Östman

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

BACKGROUND: A better definition of biomarkers and biological processes related to local recurrence and disease progression is highly warranted for ductal breast carcinoma in situ (DCIS). Stromal-epithelial interactions are likely of major importance for the biological, clinical, and pathological distinctions between high- and low-risk DCIS cases. METHODS: Stromal platelet derived growth factor receptor (PDGFR) was immunohistochemically assessed in two DCIS patient cohorts (n = 458 and n = 80). Cox proportional hazards models were used to calculate the hazard ratios of recurrence. The molecular mechanisms regulating stromal PDGFR expression were investigated in experimental in vitro co-culture systems of DCIS cells and fibroblasts and analyzed using immunoblot and quantitative real-time PCR. Knock-out of JAG1 in DCIS cells and NOTCH2 in fibroblasts was obtained through CRISPR/Cas9. Experimental data were validated by mammary fat pad injection of DCIS and DCIS-JAG1 knock-out cells (10 mice per group). All statistical tests were two-sided. RESULTS: PDGFRα(low)/PDGFRβ(high) fibroblasts were associated with increased risk for recurrence in DCIS (univariate hazard ratio = 1.59, 95% confidence interval [CI] = 1.02 to 2.46; P = .04 Wald test; multivariable hazard ratio = 1.78, 95% CI = 1.07 to 2.97; P = .03). Tissue culture and mouse model studies indicated that this fibroblast phenotype is induced by DCIS cells in a cell contact-dependent manner. Epithelial Jagged1 and fibroblast Notch2 were identified through loss-of-function studies as key juxtacrine signaling components driving the formation of the poor prognosis-associated fibroblast phenotype. CONCLUSIONS: A PDGFRα(low)/PDGFRβ(high) fibroblast subset was identified as a marker for high-risk DCIS. The Jagged-1/Notch2/PDGFR stroma-epithelial pathway was described as a novel signaling mechanism regulating this poor prognosis-associated fibroblast subset. In general terms, the study highlights epithelial-stromal crosstalk in DCIS and contributes to ongoing efforts to define clinically relevant fibroblast subsets and their etiology.

Original languageEnglish (US)
Pages (from-to)983-995
Number of pages13
JournalJournal of the National Cancer Institute
Volume111
Issue number9
DOIs
StatePublished - Sep 1 2019

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Carcinoma, Intraductal, Noninfiltrating
Platelet-Derived Growth Factor Receptors
Fibroblasts
Recurrence
Breast Carcinoma In Situ
Clustered Regularly Interspaced Short Palindromic Repeats
Confidence Intervals
Carcinoma, Ductal, Breast
Phenotype
Biological Phenomena
Coculture Techniques
Proportional Hazards Models
Disease Progression
Adipose Tissue
Real-Time Polymerase Chain Reaction
Breast
Biomarkers

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Strell, C., Paulsson, J., Jin, S. B., Tobin, N. P., Mezheyeuski, A., Roswall, P., ... Östman, A. (2019). Impact of Epithelial-Stromal Interactions on Peritumoral Fibroblasts in Ductal Carcinoma in Situ. Journal of the National Cancer Institute, 111(9), 983-995. https://doi.org/10.1093/jnci/djy234

Impact of Epithelial-Stromal Interactions on Peritumoral Fibroblasts in Ductal Carcinoma in Situ. / Strell, Carina; Paulsson, Janna; Jin, Shao Bo; Tobin, Nicholas P.; Mezheyeuski, Artur; Roswall, Pernilla; Mutgan, Ceren; Mitsios, Nicholas; Johansson, Hemming; Wickberg, Sarah Marie; Svedlund, Jessica; Nilsson, Mats; Hall, Per; Mulder, Jan; Radisky, Derek C.; Pietras, Kristian; Bergh, Jonas; Lendahl, Urban; Wärnberg, Fredrik; Östman, Arne.

In: Journal of the National Cancer Institute, Vol. 111, No. 9, 01.09.2019, p. 983-995.

Research output: Contribution to journalArticle

Strell, C, Paulsson, J, Jin, SB, Tobin, NP, Mezheyeuski, A, Roswall, P, Mutgan, C, Mitsios, N, Johansson, H, Wickberg, SM, Svedlund, J, Nilsson, M, Hall, P, Mulder, J, Radisky, DC, Pietras, K, Bergh, J, Lendahl, U, Wärnberg, F & Östman, A 2019, 'Impact of Epithelial-Stromal Interactions on Peritumoral Fibroblasts in Ductal Carcinoma in Situ', Journal of the National Cancer Institute, vol. 111, no. 9, pp. 983-995. https://doi.org/10.1093/jnci/djy234
Strell, Carina ; Paulsson, Janna ; Jin, Shao Bo ; Tobin, Nicholas P. ; Mezheyeuski, Artur ; Roswall, Pernilla ; Mutgan, Ceren ; Mitsios, Nicholas ; Johansson, Hemming ; Wickberg, Sarah Marie ; Svedlund, Jessica ; Nilsson, Mats ; Hall, Per ; Mulder, Jan ; Radisky, Derek C. ; Pietras, Kristian ; Bergh, Jonas ; Lendahl, Urban ; Wärnberg, Fredrik ; Östman, Arne. / Impact of Epithelial-Stromal Interactions on Peritumoral Fibroblasts in Ductal Carcinoma in Situ. In: Journal of the National Cancer Institute. 2019 ; Vol. 111, No. 9. pp. 983-995.
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abstract = "BACKGROUND: A better definition of biomarkers and biological processes related to local recurrence and disease progression is highly warranted for ductal breast carcinoma in situ (DCIS). Stromal-epithelial interactions are likely of major importance for the biological, clinical, and pathological distinctions between high- and low-risk DCIS cases. METHODS: Stromal platelet derived growth factor receptor (PDGFR) was immunohistochemically assessed in two DCIS patient cohorts (n = 458 and n = 80). Cox proportional hazards models were used to calculate the hazard ratios of recurrence. The molecular mechanisms regulating stromal PDGFR expression were investigated in experimental in vitro co-culture systems of DCIS cells and fibroblasts and analyzed using immunoblot and quantitative real-time PCR. Knock-out of JAG1 in DCIS cells and NOTCH2 in fibroblasts was obtained through CRISPR/Cas9. Experimental data were validated by mammary fat pad injection of DCIS and DCIS-JAG1 knock-out cells (10 mice per group). All statistical tests were two-sided. RESULTS: PDGFRα(low)/PDGFRβ(high) fibroblasts were associated with increased risk for recurrence in DCIS (univariate hazard ratio = 1.59, 95{\%} confidence interval [CI] = 1.02 to 2.46; P = .04 Wald test; multivariable hazard ratio = 1.78, 95{\%} CI = 1.07 to 2.97; P = .03). Tissue culture and mouse model studies indicated that this fibroblast phenotype is induced by DCIS cells in a cell contact-dependent manner. Epithelial Jagged1 and fibroblast Notch2 were identified through loss-of-function studies as key juxtacrine signaling components driving the formation of the poor prognosis-associated fibroblast phenotype. CONCLUSIONS: A PDGFRα(low)/PDGFRβ(high) fibroblast subset was identified as a marker for high-risk DCIS. The Jagged-1/Notch2/PDGFR stroma-epithelial pathway was described as a novel signaling mechanism regulating this poor prognosis-associated fibroblast subset. In general terms, the study highlights epithelial-stromal crosstalk in DCIS and contributes to ongoing efforts to define clinically relevant fibroblast subsets and their etiology.",
author = "Carina Strell and Janna Paulsson and Jin, {Shao Bo} and Tobin, {Nicholas P.} and Artur Mezheyeuski and Pernilla Roswall and Ceren Mutgan and Nicholas Mitsios and Hemming Johansson and Wickberg, {Sarah Marie} and Jessica Svedlund and Mats Nilsson and Per Hall and Jan Mulder and Radisky, {Derek C.} and Kristian Pietras and Jonas Bergh and Urban Lendahl and Fredrik W{\"a}rnberg and Arne {\"O}stman",
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T1 - Impact of Epithelial-Stromal Interactions on Peritumoral Fibroblasts in Ductal Carcinoma in Situ

AU - Strell, Carina

AU - Paulsson, Janna

AU - Jin, Shao Bo

AU - Tobin, Nicholas P.

AU - Mezheyeuski, Artur

AU - Roswall, Pernilla

AU - Mutgan, Ceren

AU - Mitsios, Nicholas

AU - Johansson, Hemming

AU - Wickberg, Sarah Marie

AU - Svedlund, Jessica

AU - Nilsson, Mats

AU - Hall, Per

AU - Mulder, Jan

AU - Radisky, Derek C.

AU - Pietras, Kristian

AU - Bergh, Jonas

AU - Lendahl, Urban

AU - Wärnberg, Fredrik

AU - Östman, Arne

PY - 2019/9/1

Y1 - 2019/9/1

N2 - BACKGROUND: A better definition of biomarkers and biological processes related to local recurrence and disease progression is highly warranted for ductal breast carcinoma in situ (DCIS). Stromal-epithelial interactions are likely of major importance for the biological, clinical, and pathological distinctions between high- and low-risk DCIS cases. METHODS: Stromal platelet derived growth factor receptor (PDGFR) was immunohistochemically assessed in two DCIS patient cohorts (n = 458 and n = 80). Cox proportional hazards models were used to calculate the hazard ratios of recurrence. The molecular mechanisms regulating stromal PDGFR expression were investigated in experimental in vitro co-culture systems of DCIS cells and fibroblasts and analyzed using immunoblot and quantitative real-time PCR. Knock-out of JAG1 in DCIS cells and NOTCH2 in fibroblasts was obtained through CRISPR/Cas9. Experimental data were validated by mammary fat pad injection of DCIS and DCIS-JAG1 knock-out cells (10 mice per group). All statistical tests were two-sided. RESULTS: PDGFRα(low)/PDGFRβ(high) fibroblasts were associated with increased risk for recurrence in DCIS (univariate hazard ratio = 1.59, 95% confidence interval [CI] = 1.02 to 2.46; P = .04 Wald test; multivariable hazard ratio = 1.78, 95% CI = 1.07 to 2.97; P = .03). Tissue culture and mouse model studies indicated that this fibroblast phenotype is induced by DCIS cells in a cell contact-dependent manner. Epithelial Jagged1 and fibroblast Notch2 were identified through loss-of-function studies as key juxtacrine signaling components driving the formation of the poor prognosis-associated fibroblast phenotype. CONCLUSIONS: A PDGFRα(low)/PDGFRβ(high) fibroblast subset was identified as a marker for high-risk DCIS. The Jagged-1/Notch2/PDGFR stroma-epithelial pathway was described as a novel signaling mechanism regulating this poor prognosis-associated fibroblast subset. In general terms, the study highlights epithelial-stromal crosstalk in DCIS and contributes to ongoing efforts to define clinically relevant fibroblast subsets and their etiology.

AB - BACKGROUND: A better definition of biomarkers and biological processes related to local recurrence and disease progression is highly warranted for ductal breast carcinoma in situ (DCIS). Stromal-epithelial interactions are likely of major importance for the biological, clinical, and pathological distinctions between high- and low-risk DCIS cases. METHODS: Stromal platelet derived growth factor receptor (PDGFR) was immunohistochemically assessed in two DCIS patient cohorts (n = 458 and n = 80). Cox proportional hazards models were used to calculate the hazard ratios of recurrence. The molecular mechanisms regulating stromal PDGFR expression were investigated in experimental in vitro co-culture systems of DCIS cells and fibroblasts and analyzed using immunoblot and quantitative real-time PCR. Knock-out of JAG1 in DCIS cells and NOTCH2 in fibroblasts was obtained through CRISPR/Cas9. Experimental data were validated by mammary fat pad injection of DCIS and DCIS-JAG1 knock-out cells (10 mice per group). All statistical tests were two-sided. RESULTS: PDGFRα(low)/PDGFRβ(high) fibroblasts were associated with increased risk for recurrence in DCIS (univariate hazard ratio = 1.59, 95% confidence interval [CI] = 1.02 to 2.46; P = .04 Wald test; multivariable hazard ratio = 1.78, 95% CI = 1.07 to 2.97; P = .03). Tissue culture and mouse model studies indicated that this fibroblast phenotype is induced by DCIS cells in a cell contact-dependent manner. Epithelial Jagged1 and fibroblast Notch2 were identified through loss-of-function studies as key juxtacrine signaling components driving the formation of the poor prognosis-associated fibroblast phenotype. CONCLUSIONS: A PDGFRα(low)/PDGFRβ(high) fibroblast subset was identified as a marker for high-risk DCIS. The Jagged-1/Notch2/PDGFR stroma-epithelial pathway was described as a novel signaling mechanism regulating this poor prognosis-associated fibroblast subset. In general terms, the study highlights epithelial-stromal crosstalk in DCIS and contributes to ongoing efforts to define clinically relevant fibroblast subsets and their etiology.

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