Impact of endurance training on murine spontaneous activity, muscle mitochondrial DNA abundance, gene transcripts, and function

Lisa S. Chow, Laura J. Greenlund, Yan Asmann, Kevin R. Short, Shelly K. McCrady, James A. Levine, K Sreekumaran Nair

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

We hypothesized that enhanced skeletal muscle mitochondrial function following aerobic exercise training is related to an increase in mitochondrial transcription factors, DNA abundance [mitochondrial DNA (mtDNA)], and mitochondria-related gene transcript levels, as well as spontaneous physical activity (SPA) levels. We report the effects of daily treadmill training on 12-wk-old FVB mice for 5 days/wk over 8 wk at 80% peak O2 consumption and studied the training effect on changes in body composition, glucose tolerance, muscle mtDNA muscle, mitochondria-related gene transcripts, in vitro muscle mitochondrial ATP production capacity (MATPC), and SPA levels. Compared with the untrained mice, the trained mice had higher peak O2 consumption (+18%; P < 0.001), lower percentage of abdominal (-25.4%; P < 0.02) and body fat (-19.5%; P < 0.01), improved glucose tolerance (P < 0.04), and higher muscle mitochondrial enzyme activity (+19.5- 43.8%; P < 0.04) and MATPC (+28.9 to +32.4%; P < 0.01). Gene array analysis showed significant differences in mRNAs of mitochondria-related ontology groups between the trained and untrained mice. Training also increased muscle mtDNA (+88.4 to +110%; P < 0.05), peroxisome proliferative-activated receptor-γ coactivator-1α protein (+99.5%; P < 0.04), and mitochondrial transcription factor A mRNA levels (+21.7%; P < 0.004) levels. SPA levels were higher in trained mice (P =- 0.056, two-sided t-test) and significantly correlated with two separate substrate-based measurements of MATPC (P < 0.02). In conclusion, aerobic exercise training enhances muscle mitochondrial transcription factors, mtDNA abundance, mitochondria-related gene transcript levels, and mitochondrial function, and this enhancement in mitochondrial function occurs in association with increased SPA.

Original languageEnglish (US)
Pages (from-to)1078-1089
Number of pages12
JournalJournal of Applied Physiology
Volume102
Issue number3
DOIs
StatePublished - Mar 2007

Fingerprint

Mitochondrial DNA
Muscles
Exercise
Mitochondria
Adenosine Triphosphate
Genes
Transcription Factors
Muscle Mitochondrion
Glucose
Messenger RNA
Peroxisomes
Body Composition
Adipose Tissue
Skeletal Muscle
DNA
Enzymes
Proteins

Keywords

  • Endurance exercise
  • Gene array
  • Mitochondria adenosine 5′-triphosphate production
  • Mouse
  • Physical activity

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Impact of endurance training on murine spontaneous activity, muscle mitochondrial DNA abundance, gene transcripts, and function. / Chow, Lisa S.; Greenlund, Laura J.; Asmann, Yan; Short, Kevin R.; McCrady, Shelly K.; Levine, James A.; Nair, K Sreekumaran.

In: Journal of Applied Physiology, Vol. 102, No. 3, 03.2007, p. 1078-1089.

Research output: Contribution to journalArticle

Chow, Lisa S. ; Greenlund, Laura J. ; Asmann, Yan ; Short, Kevin R. ; McCrady, Shelly K. ; Levine, James A. ; Nair, K Sreekumaran. / Impact of endurance training on murine spontaneous activity, muscle mitochondrial DNA abundance, gene transcripts, and function. In: Journal of Applied Physiology. 2007 ; Vol. 102, No. 3. pp. 1078-1089.
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abstract = "We hypothesized that enhanced skeletal muscle mitochondrial function following aerobic exercise training is related to an increase in mitochondrial transcription factors, DNA abundance [mitochondrial DNA (mtDNA)], and mitochondria-related gene transcript levels, as well as spontaneous physical activity (SPA) levels. We report the effects of daily treadmill training on 12-wk-old FVB mice for 5 days/wk over 8 wk at 80{\%} peak O2 consumption and studied the training effect on changes in body composition, glucose tolerance, muscle mtDNA muscle, mitochondria-related gene transcripts, in vitro muscle mitochondrial ATP production capacity (MATPC), and SPA levels. Compared with the untrained mice, the trained mice had higher peak O2 consumption (+18{\%}; P < 0.001), lower percentage of abdominal (-25.4{\%}; P < 0.02) and body fat (-19.5{\%}; P < 0.01), improved glucose tolerance (P < 0.04), and higher muscle mitochondrial enzyme activity (+19.5- 43.8{\%}; P < 0.04) and MATPC (+28.9 to +32.4{\%}; P < 0.01). Gene array analysis showed significant differences in mRNAs of mitochondria-related ontology groups between the trained and untrained mice. Training also increased muscle mtDNA (+88.4 to +110{\%}; P < 0.05), peroxisome proliferative-activated receptor-γ coactivator-1α protein (+99.5{\%}; P < 0.04), and mitochondrial transcription factor A mRNA levels (+21.7{\%}; P < 0.004) levels. SPA levels were higher in trained mice (P =- 0.056, two-sided t-test) and significantly correlated with two separate substrate-based measurements of MATPC (P < 0.02). In conclusion, aerobic exercise training enhances muscle mitochondrial transcription factors, mtDNA abundance, mitochondria-related gene transcript levels, and mitochondrial function, and this enhancement in mitochondrial function occurs in association with increased SPA.",
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