Impact of ascorbic acid supplementation on haematological response and erythrocyte antioxidant enzyme systems during ageing in rats

Muthuvel Jayachandran, Chinnakkannu Panneerselvam

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Haematological response and erythrocyte antioxidant activity were evaluated in young and aged rats fed a normal diet and with ascorbic acid supplementation (20 mg/100 g body weight) for 15, 30 and 60 days. Reticulocyte count, haemoglobin concentration, activities of superoxide dismutase, catalase and glutathione peroxidase, and level of blood ascorbic acid, were markedly low in aged rats while lipid peroxidation was significantly high as compared with young rats. In contrast, there was a considerable decrease in lipid peroxidation after 30 and 60 days ascorbic acid supplementation while the levels of red blood cells, reticulocytes, haemoglobin, packed cell volume and mean cell haemoglobin concentration and activities of antioxidant enzymes increased markedly after 30 and 60 days of supplementation in aged rats. In young rats, red blood cells, haemoglobin and the activities of antioxidant enzymes increased only after 60 days of ascorbic acid fortification. However, mean cell haemoglobin concentration had increased significantly even after 15 days supplementation with ascorbic acid.

Original languageEnglish (US)
Pages (from-to)65-67
Number of pages3
JournalMedical Science Research
Issue number1
StatePublished - Mar 7 1997



  • Ageing
  • Antioxidant enzymes
  • Ascorbic acid
  • Free radicals
  • Haematological response
  • Lipid peroxidation
  • Oxidative damage

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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