Impact of aging on diaphragm muscle function in male and female Fischer 344 rats

Obaid U. Khurram, Matthew J. Fogarty, Tiffany L. Sarrafian, Arjun Bhatt, Carlos Bernardo Mantilla, Gary C Sieck

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The diaphragm muscle (DIAm) is the primary inspiratory muscle in mammals and is active during ventilatory behaviors, but it is also involved in higher-force behaviors such as those necessary for clearing the airway. Our laboratory has previously reported DIAm sarcopenia in rats and mice characterized by DIAm atrophy and a reduction in maximum specific force at 24 months of age. In Fischer 344 rats, these studies were limited to male animals, although in other studies, we noted a more rapid increase in body mass from 6 to 24 months of age in females (~140%) compared to males (~110%). This difference in body weight gain suggests a possible sex difference in the manifestation of sarcopenia. In mice, we previously measured transdiaphragmatic pressure (Pdi) to evaluate in vivo DIAm force generation across a range of motor behaviors, but found no evidence of sex-related differences. The purpose of this study in Fischer 344 rats was to evaluate if there are sex-related differences in DIAm sarcopenia, and if such differences translate to a functional impact on Pdi generation across motor behaviors and maximal Pdi (Pdimax) elicited by bilateral phrenic nerve stimulation. In both males and females, DIAm sarcopenia was apparent in 24-month-old rats with a ~30% reduction in both maximum specific force and the cross-sectional area of type IIx and/or IIb fibers. Importantly, in both males and females, Pdi generated during ventilatory behaviors was unimpaired by sarcopenia, even during more forceful ventilatory efforts induced via airway occlusion. Although ventilatory behaviors were preserved with aging, there was a ~20% reduction in Pdimax, which likely impairs the ability of the DIAm to generate higher-force expulsive airway clearance behaviors necessary to maintain airway patency.

Original languageEnglish (US)
Article numbere13786
JournalPhysiological Reports
Volume6
Issue number13
DOIs
StatePublished - Jul 1 2018

Fingerprint

Inbred F344 Rats
Diaphragm
Sarcopenia
Muscles
Sex Characteristics
Phrenic Nerve
Aptitude
Muscular Atrophy
Weight Gain
Mammals
Body Weight
Pressure

Keywords

  • Aging
  • diaphragm muscle
  • fiber type
  • Sarcopenia
  • sex differences
  • transdiaphragmatic pressure

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Khurram, O. U., Fogarty, M. J., Sarrafian, T. L., Bhatt, A., Mantilla, C. B., & Sieck, G. C. (2018). Impact of aging on diaphragm muscle function in male and female Fischer 344 rats. Physiological Reports, 6(13), [e13786]. https://doi.org/10.14814/phy2.13786

Impact of aging on diaphragm muscle function in male and female Fischer 344 rats. / Khurram, Obaid U.; Fogarty, Matthew J.; Sarrafian, Tiffany L.; Bhatt, Arjun; Mantilla, Carlos Bernardo; Sieck, Gary C.

In: Physiological Reports, Vol. 6, No. 13, e13786, 01.07.2018.

Research output: Contribution to journalArticle

Khurram, Obaid U. ; Fogarty, Matthew J. ; Sarrafian, Tiffany L. ; Bhatt, Arjun ; Mantilla, Carlos Bernardo ; Sieck, Gary C. / Impact of aging on diaphragm muscle function in male and female Fischer 344 rats. In: Physiological Reports. 2018 ; Vol. 6, No. 13.
@article{da9f328a86de4bbe9a1a9270433ff02e,
title = "Impact of aging on diaphragm muscle function in male and female Fischer 344 rats",
abstract = "The diaphragm muscle (DIAm) is the primary inspiratory muscle in mammals and is active during ventilatory behaviors, but it is also involved in higher-force behaviors such as those necessary for clearing the airway. Our laboratory has previously reported DIAm sarcopenia in rats and mice characterized by DIAm atrophy and a reduction in maximum specific force at 24 months of age. In Fischer 344 rats, these studies were limited to male animals, although in other studies, we noted a more rapid increase in body mass from 6 to 24 months of age in females (~140{\%}) compared to males (~110{\%}). This difference in body weight gain suggests a possible sex difference in the manifestation of sarcopenia. In mice, we previously measured transdiaphragmatic pressure (Pdi) to evaluate in vivo DIAm force generation across a range of motor behaviors, but found no evidence of sex-related differences. The purpose of this study in Fischer 344 rats was to evaluate if there are sex-related differences in DIAm sarcopenia, and if such differences translate to a functional impact on Pdi generation across motor behaviors and maximal Pdi (Pdimax) elicited by bilateral phrenic nerve stimulation. In both males and females, DIAm sarcopenia was apparent in 24-month-old rats with a ~30{\%} reduction in both maximum specific force and the cross-sectional area of type IIx and/or IIb fibers. Importantly, in both males and females, Pdi generated during ventilatory behaviors was unimpaired by sarcopenia, even during more forceful ventilatory efforts induced via airway occlusion. Although ventilatory behaviors were preserved with aging, there was a ~20{\%} reduction in Pdimax, which likely impairs the ability of the DIAm to generate higher-force expulsive airway clearance behaviors necessary to maintain airway patency.",
keywords = "Aging, diaphragm muscle, fiber type, Sarcopenia, sex differences, transdiaphragmatic pressure",
author = "Khurram, {Obaid U.} and Fogarty, {Matthew J.} and Sarrafian, {Tiffany L.} and Arjun Bhatt and Mantilla, {Carlos Bernardo} and Sieck, {Gary C}",
year = "2018",
month = "7",
day = "1",
doi = "10.14814/phy2.13786",
language = "English (US)",
volume = "6",
journal = "Physiological Reports",
issn = "2051-817X",
publisher = "John Wiley and Sons Inc.",
number = "13",

}

TY - JOUR

T1 - Impact of aging on diaphragm muscle function in male and female Fischer 344 rats

AU - Khurram, Obaid U.

AU - Fogarty, Matthew J.

AU - Sarrafian, Tiffany L.

AU - Bhatt, Arjun

AU - Mantilla, Carlos Bernardo

AU - Sieck, Gary C

PY - 2018/7/1

Y1 - 2018/7/1

N2 - The diaphragm muscle (DIAm) is the primary inspiratory muscle in mammals and is active during ventilatory behaviors, but it is also involved in higher-force behaviors such as those necessary for clearing the airway. Our laboratory has previously reported DIAm sarcopenia in rats and mice characterized by DIAm atrophy and a reduction in maximum specific force at 24 months of age. In Fischer 344 rats, these studies were limited to male animals, although in other studies, we noted a more rapid increase in body mass from 6 to 24 months of age in females (~140%) compared to males (~110%). This difference in body weight gain suggests a possible sex difference in the manifestation of sarcopenia. In mice, we previously measured transdiaphragmatic pressure (Pdi) to evaluate in vivo DIAm force generation across a range of motor behaviors, but found no evidence of sex-related differences. The purpose of this study in Fischer 344 rats was to evaluate if there are sex-related differences in DIAm sarcopenia, and if such differences translate to a functional impact on Pdi generation across motor behaviors and maximal Pdi (Pdimax) elicited by bilateral phrenic nerve stimulation. In both males and females, DIAm sarcopenia was apparent in 24-month-old rats with a ~30% reduction in both maximum specific force and the cross-sectional area of type IIx and/or IIb fibers. Importantly, in both males and females, Pdi generated during ventilatory behaviors was unimpaired by sarcopenia, even during more forceful ventilatory efforts induced via airway occlusion. Although ventilatory behaviors were preserved with aging, there was a ~20% reduction in Pdimax, which likely impairs the ability of the DIAm to generate higher-force expulsive airway clearance behaviors necessary to maintain airway patency.

AB - The diaphragm muscle (DIAm) is the primary inspiratory muscle in mammals and is active during ventilatory behaviors, but it is also involved in higher-force behaviors such as those necessary for clearing the airway. Our laboratory has previously reported DIAm sarcopenia in rats and mice characterized by DIAm atrophy and a reduction in maximum specific force at 24 months of age. In Fischer 344 rats, these studies were limited to male animals, although in other studies, we noted a more rapid increase in body mass from 6 to 24 months of age in females (~140%) compared to males (~110%). This difference in body weight gain suggests a possible sex difference in the manifestation of sarcopenia. In mice, we previously measured transdiaphragmatic pressure (Pdi) to evaluate in vivo DIAm force generation across a range of motor behaviors, but found no evidence of sex-related differences. The purpose of this study in Fischer 344 rats was to evaluate if there are sex-related differences in DIAm sarcopenia, and if such differences translate to a functional impact on Pdi generation across motor behaviors and maximal Pdi (Pdimax) elicited by bilateral phrenic nerve stimulation. In both males and females, DIAm sarcopenia was apparent in 24-month-old rats with a ~30% reduction in both maximum specific force and the cross-sectional area of type IIx and/or IIb fibers. Importantly, in both males and females, Pdi generated during ventilatory behaviors was unimpaired by sarcopenia, even during more forceful ventilatory efforts induced via airway occlusion. Although ventilatory behaviors were preserved with aging, there was a ~20% reduction in Pdimax, which likely impairs the ability of the DIAm to generate higher-force expulsive airway clearance behaviors necessary to maintain airway patency.

KW - Aging

KW - diaphragm muscle

KW - fiber type

KW - Sarcopenia

KW - sex differences

KW - transdiaphragmatic pressure

UR - http://www.scopus.com/inward/record.url?scp=85049803835&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049803835&partnerID=8YFLogxK

U2 - 10.14814/phy2.13786

DO - 10.14814/phy2.13786

M3 - Article

C2 - 29981218

AN - SCOPUS:85049803835

VL - 6

JO - Physiological Reports

JF - Physiological Reports

SN - 2051-817X

IS - 13

M1 - e13786

ER -