Immunophenotyping of chimeric cells in localized scleroderma

K. T. McNallan, C. Aponte, R. el-Azhary, Thomas Mason, A. M. Nelson, J. J. Paat, Cynthia Crowson, Ann M. Reed

Research output: Contribution to journalArticle

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Abstract

Objective. Localized scleroderma causes thickening of the skin due to excessive collagen deposition. This condition has clinical and histopathological similarities to chronic graft- vs -host disease. We wanted to identify whether chimeric cells are present in the affected tissue in localized scleroderma and to further investigate the role of chimerism by immunophenotyping the chimeric cells. We hypothesize that the presence of chimerism and immunotypic chimeric cells will lend to an understanding of the pathogenesis of localized scleroderma and possible mechanisms by which chimeric cells participate in autoimmunity. Methods. We studied skin biopsies from 18 localized scleroderma patients and compared them with concurrent biopsies from unaffected skin in a subset of patients. Skin biopsies from morphoea and linear scleroderma patients were analysed for the presence of chimeric cells using male-female (X, Y) differences. Cell surface markers (CD4, CD8, CD19/20, CD68, S100, CD14 and CD56) were determined for cell phenotyping of chimeric cells. Results. Overall, the affected tissue contained a greater number of lymphocytic inflammatory cells. In the affected tissue, 38% of the total chimeric cells were CD68+ (dendritic cell, monocyte and macrophage marker), 29% Langerin/S100+ (dendritic cell marker), 26% CD8+ (cytotoxic T-lymphocyte marker), 20% CD19/20+ (B-lymphocyte marker), 14% CD4+ (T-helper lymphocyte) and 0% CD56+ (natural killer cell marker). Conclusions. We report that not only are chimeric cells present in affected localized scleroderma lesions but they also are more likely to be dendritic cells and B lymphocytes suggesting a role in the pathogenesis of localized scleroderma.

Original languageEnglish (US)
Pages (from-to)398-402
Number of pages5
JournalRheumatology
Volume46
Issue number3
DOIs
StatePublished - Mar 2007

Fingerprint

Localized Scleroderma
Immunophenotyping
Dendritic Cells
Skin
Chimerism
Biopsy
B-Lymphocytes
Cytotoxic T-Lymphocytes
Graft vs Host Disease
Helper-Inducer T-Lymphocytes
Autoimmunity
Natural Killer Cells
Monocytes
Collagen

Keywords

  • cGVHD
  • Chimerism
  • Localized scleroderma
  • Morphoea

ASJC Scopus subject areas

  • Neuroscience(all)
  • Rheumatology

Cite this

McNallan, K. T., Aponte, C., el-Azhary, R., Mason, T., Nelson, A. M., Paat, J. J., ... Reed, A. M. (2007). Immunophenotyping of chimeric cells in localized scleroderma. Rheumatology, 46(3), 398-402. https://doi.org/10.1093/rheumatology/kel297

Immunophenotyping of chimeric cells in localized scleroderma. / McNallan, K. T.; Aponte, C.; el-Azhary, R.; Mason, Thomas; Nelson, A. M.; Paat, J. J.; Crowson, Cynthia; Reed, Ann M.

In: Rheumatology, Vol. 46, No. 3, 03.2007, p. 398-402.

Research output: Contribution to journalArticle

McNallan, KT, Aponte, C, el-Azhary, R, Mason, T, Nelson, AM, Paat, JJ, Crowson, C & Reed, AM 2007, 'Immunophenotyping of chimeric cells in localized scleroderma', Rheumatology, vol. 46, no. 3, pp. 398-402. https://doi.org/10.1093/rheumatology/kel297
McNallan KT, Aponte C, el-Azhary R, Mason T, Nelson AM, Paat JJ et al. Immunophenotyping of chimeric cells in localized scleroderma. Rheumatology. 2007 Mar;46(3):398-402. https://doi.org/10.1093/rheumatology/kel297
McNallan, K. T. ; Aponte, C. ; el-Azhary, R. ; Mason, Thomas ; Nelson, A. M. ; Paat, J. J. ; Crowson, Cynthia ; Reed, Ann M. / Immunophenotyping of chimeric cells in localized scleroderma. In: Rheumatology. 2007 ; Vol. 46, No. 3. pp. 398-402.
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abstract = "Objective. Localized scleroderma causes thickening of the skin due to excessive collagen deposition. This condition has clinical and histopathological similarities to chronic graft- vs -host disease. We wanted to identify whether chimeric cells are present in the affected tissue in localized scleroderma and to further investigate the role of chimerism by immunophenotyping the chimeric cells. We hypothesize that the presence of chimerism and immunotypic chimeric cells will lend to an understanding of the pathogenesis of localized scleroderma and possible mechanisms by which chimeric cells participate in autoimmunity. Methods. We studied skin biopsies from 18 localized scleroderma patients and compared them with concurrent biopsies from unaffected skin in a subset of patients. Skin biopsies from morphoea and linear scleroderma patients were analysed for the presence of chimeric cells using male-female (X, Y) differences. Cell surface markers (CD4, CD8, CD19/20, CD68, S100, CD14 and CD56) were determined for cell phenotyping of chimeric cells. Results. Overall, the affected tissue contained a greater number of lymphocytic inflammatory cells. In the affected tissue, 38{\%} of the total chimeric cells were CD68+ (dendritic cell, monocyte and macrophage marker), 29{\%} Langerin/S100+ (dendritic cell marker), 26{\%} CD8+ (cytotoxic T-lymphocyte marker), 20{\%} CD19/20+ (B-lymphocyte marker), 14{\%} CD4+ (T-helper lymphocyte) and 0{\%} CD56+ (natural killer cell marker). Conclusions. We report that not only are chimeric cells present in affected localized scleroderma lesions but they also are more likely to be dendritic cells and B lymphocytes suggesting a role in the pathogenesis of localized scleroderma.",
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AU - Aponte, C.

AU - el-Azhary, R.

AU - Mason, Thomas

AU - Nelson, A. M.

AU - Paat, J. J.

AU - Crowson, Cynthia

AU - Reed, Ann M.

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N2 - Objective. Localized scleroderma causes thickening of the skin due to excessive collagen deposition. This condition has clinical and histopathological similarities to chronic graft- vs -host disease. We wanted to identify whether chimeric cells are present in the affected tissue in localized scleroderma and to further investigate the role of chimerism by immunophenotyping the chimeric cells. We hypothesize that the presence of chimerism and immunotypic chimeric cells will lend to an understanding of the pathogenesis of localized scleroderma and possible mechanisms by which chimeric cells participate in autoimmunity. Methods. We studied skin biopsies from 18 localized scleroderma patients and compared them with concurrent biopsies from unaffected skin in a subset of patients. Skin biopsies from morphoea and linear scleroderma patients were analysed for the presence of chimeric cells using male-female (X, Y) differences. Cell surface markers (CD4, CD8, CD19/20, CD68, S100, CD14 and CD56) were determined for cell phenotyping of chimeric cells. Results. Overall, the affected tissue contained a greater number of lymphocytic inflammatory cells. In the affected tissue, 38% of the total chimeric cells were CD68+ (dendritic cell, monocyte and macrophage marker), 29% Langerin/S100+ (dendritic cell marker), 26% CD8+ (cytotoxic T-lymphocyte marker), 20% CD19/20+ (B-lymphocyte marker), 14% CD4+ (T-helper lymphocyte) and 0% CD56+ (natural killer cell marker). Conclusions. We report that not only are chimeric cells present in affected localized scleroderma lesions but they also are more likely to be dendritic cells and B lymphocytes suggesting a role in the pathogenesis of localized scleroderma.

AB - Objective. Localized scleroderma causes thickening of the skin due to excessive collagen deposition. This condition has clinical and histopathological similarities to chronic graft- vs -host disease. We wanted to identify whether chimeric cells are present in the affected tissue in localized scleroderma and to further investigate the role of chimerism by immunophenotyping the chimeric cells. We hypothesize that the presence of chimerism and immunotypic chimeric cells will lend to an understanding of the pathogenesis of localized scleroderma and possible mechanisms by which chimeric cells participate in autoimmunity. Methods. We studied skin biopsies from 18 localized scleroderma patients and compared them with concurrent biopsies from unaffected skin in a subset of patients. Skin biopsies from morphoea and linear scleroderma patients were analysed for the presence of chimeric cells using male-female (X, Y) differences. Cell surface markers (CD4, CD8, CD19/20, CD68, S100, CD14 and CD56) were determined for cell phenotyping of chimeric cells. Results. Overall, the affected tissue contained a greater number of lymphocytic inflammatory cells. In the affected tissue, 38% of the total chimeric cells were CD68+ (dendritic cell, monocyte and macrophage marker), 29% Langerin/S100+ (dendritic cell marker), 26% CD8+ (cytotoxic T-lymphocyte marker), 20% CD19/20+ (B-lymphocyte marker), 14% CD4+ (T-helper lymphocyte) and 0% CD56+ (natural killer cell marker). Conclusions. We report that not only are chimeric cells present in affected localized scleroderma lesions but they also are more likely to be dendritic cells and B lymphocytes suggesting a role in the pathogenesis of localized scleroderma.

KW - cGVHD

KW - Chimerism

KW - Localized scleroderma

KW - Morphoea

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