Immunophenotype of pleomorphic xanthoastrocytoma

Caterina Giannini, Bernd W. Scheithauer, Maria B S Lopes, Takanori Hirose, Johan M. Kros, Scott R. VandenBerg

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Abstract

Pleomorphic xanthoastrocytoma (PXA) is an uncommon tumor, often seizure-associated and occurring in the temporal lobe of young adults. Although its cells are considered astrocytic in nature, recent studies suggest the presence of neuronal differentiation and a possible relationship to glioneuronal neoplasms. We immunostained 40 cases of PXA, including two composite PXA-gangliogliomas (PXA-GG), with a panel of glial (glial fibrillary acidic protein, S-100 protein) and neuronal markers (class III β-tubulin, synaptophysin, neurofilament proteins, MAP2, and chromogranin A). Conventional PXAs demonstrated immunoreactivity for glial fibrillary acidic protein (100% of cases), S-100 protein (100%), class III β-tubulin (73%), synaptophysin (38%), NF proteins (18 and 8%), and MAP2 (8%). Chromogranin A stain was absent in all conventional PXA cases. Neoplastic ganglion cells in both PXA-GGs stained with class III β-tubulin, synaptophysin, and chromogranin A. Ultrastructural studies, performed in nine cases, demonstrated neuronal features including microtubules, dense core granules, and/or clear vesicles largely limited to cell processes (two PXAs) and in the cytoplasm (PXA component of one PXA-GG). Although the essential nature of PXA is clearly and uniformly glial, the significance of the limited neuronal differentiation is unclear, as it is the relationship between conventional PXA and PXA-GG. We found no evidence that the former is a precursor of the latter.

Original languageEnglish (US)
Pages (from-to)479-485
Number of pages7
JournalAmerican Journal of Surgical Pathology
Volume26
Issue number4
DOIs
StatePublished - 2002
Externally publishedYes

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Chromogranin A
Synaptophysin
Tubulin
S100 Proteins
Glial Fibrillary Acidic Protein
Neuroglia
Ganglioglioma
Neurofilament Proteins
Protein S
Temporal Lobe
Microtubules
Ganglia
Young Adult
Neoplasms
Cytoplasm
Seizures
Coloring Agents
Proteins

Keywords

  • Divergent differentiation
  • Ganglioglioma
  • Immunophenotype
  • Pleomorphic xanthoastrocytoma

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine

Cite this

Giannini, C., Scheithauer, B. W., Lopes, M. B. S., Hirose, T., Kros, J. M., & VandenBerg, S. R. (2002). Immunophenotype of pleomorphic xanthoastrocytoma. American Journal of Surgical Pathology, 26(4), 479-485. https://doi.org/10.1097/00000478-200204000-00010

Immunophenotype of pleomorphic xanthoastrocytoma. / Giannini, Caterina; Scheithauer, Bernd W.; Lopes, Maria B S; Hirose, Takanori; Kros, Johan M.; VandenBerg, Scott R.

In: American Journal of Surgical Pathology, Vol. 26, No. 4, 2002, p. 479-485.

Research output: Contribution to journalArticle

Giannini, C, Scheithauer, BW, Lopes, MBS, Hirose, T, Kros, JM & VandenBerg, SR 2002, 'Immunophenotype of pleomorphic xanthoastrocytoma', American Journal of Surgical Pathology, vol. 26, no. 4, pp. 479-485. https://doi.org/10.1097/00000478-200204000-00010
Giannini, Caterina ; Scheithauer, Bernd W. ; Lopes, Maria B S ; Hirose, Takanori ; Kros, Johan M. ; VandenBerg, Scott R. / Immunophenotype of pleomorphic xanthoastrocytoma. In: American Journal of Surgical Pathology. 2002 ; Vol. 26, No. 4. pp. 479-485.
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AU - VandenBerg, Scott R.

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