Immunologic and mass-spectrometric estimates of SHBG concentrations in healthy women

Johannes D Veldhuis, Roy B. Dyer, Sergey A. Trushin, Olga P. Bondar, Ravinder Jit Singh, George G. Klee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective Sex-hormone binding globulin (SHBG) concentrations across the adult female lifespan are not well defined. To address this knowledge gap, SHBG was quantified by both immunological and criterion methods, viz, mass spectrometry (MS). Methods Setting: Center for Translational Science Activities (CTSA). Participants: Healthy nonpregnant women (N = 120) ages 21 to 79 years. Outcomes: SHBG, testosterone (T), estradiol (E2) and estrone (E 1) each determined by MS. Uni- and multivariate regression of SHBG concentrations on age, body mass index (BMI), total and visceral abdominal fat (TAF, AVF), albumin, glucose, insulin, sex steroids, selected cytokines, blood pressure, and lipids. Results By univariate regression, MS-estimated SHBG correlated negatively with BMI, TAF, AVF, insulin, free T and bioavailable T (bio T) (each P ≤ 10-4), but not with blood pressure or lipids. By stepwise multivariate regression analysis, free and total T (both positive) and bio T (negative) were correlated with SHBG in all 4 assays (each P < 10 -15, R2 ≥ 0.481). In addition, TAF and BMI were negatively associated with SHBG (P ≤ 0.0066) in 2 SHBG assays, and estrone and IL-8 with SHBG weakly (P ≤ 0.035) in one SHBG assay each. When nonsignificant cytokines were excluded, SHBG was jointly associated with AVF, total T and HDL (P < 10-9, R2 = 0.358). Conclusion According to MS, three metabolic factors, T, AVF and HDL, together explain more than one-third of the interindividual variation in SHBG levels. We speculate that these measures reflect insulin action.

Original languageEnglish (US)
Pages (from-to)783-792
Number of pages10
JournalMetabolism: Clinical and Experimental
Volume63
Issue number6
DOIs
StatePublished - 2014

Fingerprint

Sex Hormone-Binding Globulin
Mass Spectrometry
Body Mass Index
Estrone
Insulin
Cytokines
Blood Pressure
Lipids
Intra-Abdominal Fat
Interleukin-8
Testosterone
Albumins
Estradiol
Healthy Volunteers
Multivariate Analysis
Steroids

Keywords

  • Body composition
  • Female
  • Insulin
  • Obesity
  • Sex steroid

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Immunologic and mass-spectrometric estimates of SHBG concentrations in healthy women. / Veldhuis, Johannes D; Dyer, Roy B.; Trushin, Sergey A.; Bondar, Olga P.; Singh, Ravinder Jit; Klee, George G.

In: Metabolism: Clinical and Experimental, Vol. 63, No. 6, 2014, p. 783-792.

Research output: Contribution to journalArticle

Veldhuis, Johannes D ; Dyer, Roy B. ; Trushin, Sergey A. ; Bondar, Olga P. ; Singh, Ravinder Jit ; Klee, George G. / Immunologic and mass-spectrometric estimates of SHBG concentrations in healthy women. In: Metabolism: Clinical and Experimental. 2014 ; Vol. 63, No. 6. pp. 783-792.
@article{6fb65fcb2c9a4aa898b76b72ec0eb940,
title = "Immunologic and mass-spectrometric estimates of SHBG concentrations in healthy women",
abstract = "Objective Sex-hormone binding globulin (SHBG) concentrations across the adult female lifespan are not well defined. To address this knowledge gap, SHBG was quantified by both immunological and criterion methods, viz, mass spectrometry (MS). Methods Setting: Center for Translational Science Activities (CTSA). Participants: Healthy nonpregnant women (N = 120) ages 21 to 79 years. Outcomes: SHBG, testosterone (T), estradiol (E2) and estrone (E 1) each determined by MS. Uni- and multivariate regression of SHBG concentrations on age, body mass index (BMI), total and visceral abdominal fat (TAF, AVF), albumin, glucose, insulin, sex steroids, selected cytokines, blood pressure, and lipids. Results By univariate regression, MS-estimated SHBG correlated negatively with BMI, TAF, AVF, insulin, free T and bioavailable T (bio T) (each P ≤ 10-4), but not with blood pressure or lipids. By stepwise multivariate regression analysis, free and total T (both positive) and bio T (negative) were correlated with SHBG in all 4 assays (each P < 10 -15, R2 ≥ 0.481). In addition, TAF and BMI were negatively associated with SHBG (P ≤ 0.0066) in 2 SHBG assays, and estrone and IL-8 with SHBG weakly (P ≤ 0.035) in one SHBG assay each. When nonsignificant cytokines were excluded, SHBG was jointly associated with AVF, total T and HDL (P < 10-9, R2 = 0.358). Conclusion According to MS, three metabolic factors, T, AVF and HDL, together explain more than one-third of the interindividual variation in SHBG levels. We speculate that these measures reflect insulin action.",
keywords = "Body composition, Female, Insulin, Obesity, Sex steroid",
author = "Veldhuis, {Johannes D} and Dyer, {Roy B.} and Trushin, {Sergey A.} and Bondar, {Olga P.} and Singh, {Ravinder Jit} and Klee, {George G.}",
year = "2014",
doi = "10.1016/j.metabol.2014.03.010",
language = "English (US)",
volume = "63",
pages = "783--792",
journal = "Metabolism: Clinical and Experimental",
issn = "0026-0495",
publisher = "W.B. Saunders Ltd",
number = "6",

}

TY - JOUR

T1 - Immunologic and mass-spectrometric estimates of SHBG concentrations in healthy women

AU - Veldhuis, Johannes D

AU - Dyer, Roy B.

AU - Trushin, Sergey A.

AU - Bondar, Olga P.

AU - Singh, Ravinder Jit

AU - Klee, George G.

PY - 2014

Y1 - 2014

N2 - Objective Sex-hormone binding globulin (SHBG) concentrations across the adult female lifespan are not well defined. To address this knowledge gap, SHBG was quantified by both immunological and criterion methods, viz, mass spectrometry (MS). Methods Setting: Center for Translational Science Activities (CTSA). Participants: Healthy nonpregnant women (N = 120) ages 21 to 79 years. Outcomes: SHBG, testosterone (T), estradiol (E2) and estrone (E 1) each determined by MS. Uni- and multivariate regression of SHBG concentrations on age, body mass index (BMI), total and visceral abdominal fat (TAF, AVF), albumin, glucose, insulin, sex steroids, selected cytokines, blood pressure, and lipids. Results By univariate regression, MS-estimated SHBG correlated negatively with BMI, TAF, AVF, insulin, free T and bioavailable T (bio T) (each P ≤ 10-4), but not with blood pressure or lipids. By stepwise multivariate regression analysis, free and total T (both positive) and bio T (negative) were correlated with SHBG in all 4 assays (each P < 10 -15, R2 ≥ 0.481). In addition, TAF and BMI were negatively associated with SHBG (P ≤ 0.0066) in 2 SHBG assays, and estrone and IL-8 with SHBG weakly (P ≤ 0.035) in one SHBG assay each. When nonsignificant cytokines were excluded, SHBG was jointly associated with AVF, total T and HDL (P < 10-9, R2 = 0.358). Conclusion According to MS, three metabolic factors, T, AVF and HDL, together explain more than one-third of the interindividual variation in SHBG levels. We speculate that these measures reflect insulin action.

AB - Objective Sex-hormone binding globulin (SHBG) concentrations across the adult female lifespan are not well defined. To address this knowledge gap, SHBG was quantified by both immunological and criterion methods, viz, mass spectrometry (MS). Methods Setting: Center for Translational Science Activities (CTSA). Participants: Healthy nonpregnant women (N = 120) ages 21 to 79 years. Outcomes: SHBG, testosterone (T), estradiol (E2) and estrone (E 1) each determined by MS. Uni- and multivariate regression of SHBG concentrations on age, body mass index (BMI), total and visceral abdominal fat (TAF, AVF), albumin, glucose, insulin, sex steroids, selected cytokines, blood pressure, and lipids. Results By univariate regression, MS-estimated SHBG correlated negatively with BMI, TAF, AVF, insulin, free T and bioavailable T (bio T) (each P ≤ 10-4), but not with blood pressure or lipids. By stepwise multivariate regression analysis, free and total T (both positive) and bio T (negative) were correlated with SHBG in all 4 assays (each P < 10 -15, R2 ≥ 0.481). In addition, TAF and BMI were negatively associated with SHBG (P ≤ 0.0066) in 2 SHBG assays, and estrone and IL-8 with SHBG weakly (P ≤ 0.035) in one SHBG assay each. When nonsignificant cytokines were excluded, SHBG was jointly associated with AVF, total T and HDL (P < 10-9, R2 = 0.358). Conclusion According to MS, three metabolic factors, T, AVF and HDL, together explain more than one-third of the interindividual variation in SHBG levels. We speculate that these measures reflect insulin action.

KW - Body composition

KW - Female

KW - Insulin

KW - Obesity

KW - Sex steroid

UR - http://www.scopus.com/inward/record.url?scp=84901197845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901197845&partnerID=8YFLogxK

U2 - 10.1016/j.metabol.2014.03.010

DO - 10.1016/j.metabol.2014.03.010

M3 - Article

VL - 63

SP - 783

EP - 792

JO - Metabolism: Clinical and Experimental

JF - Metabolism: Clinical and Experimental

SN - 0026-0495

IS - 6

ER -