TY - JOUR
T1 - Immunologic and mass-spectrometric estimates of SHBG concentrations in healthy women
AU - Veldhuis, Johannes D.
AU - Dyer, Roy B.
AU - Trushin, Sergey A.
AU - Bondar, Olga P.
AU - Singh, Ravinder J.
AU - Klee, George G.
N1 - Funding Information:
Supported in part via R01 DK073148 and AG029362 and P30 DK050456 (Metabolic Studies Core of the Minnesota Obesity Center) from the National Institutes of Health (Bethesda, MD). The project described was supported by UL1 TR000135 from the National Center for Advancing Translational Sciences (NCTS) and 60NANB10D005Z from the National Institute of Standards and Technology (NIST) . Contents are solely the responsibility of the authors and do not necessarily represent the official views of NIH or NIST.
PY - 2014/6
Y1 - 2014/6
N2 - Objective Sex-hormone binding globulin (SHBG) concentrations across the adult female lifespan are not well defined. To address this knowledge gap, SHBG was quantified by both immunological and criterion methods, viz, mass spectrometry (MS). Methods Setting: Center for Translational Science Activities (CTSA). Participants: Healthy nonpregnant women (N = 120) ages 21 to 79 years. Outcomes: SHBG, testosterone (T), estradiol (E2) and estrone (E 1) each determined by MS. Uni- and multivariate regression of SHBG concentrations on age, body mass index (BMI), total and visceral abdominal fat (TAF, AVF), albumin, glucose, insulin, sex steroids, selected cytokines, blood pressure, and lipids. Results By univariate regression, MS-estimated SHBG correlated negatively with BMI, TAF, AVF, insulin, free T and bioavailable T (bio T) (each P ≤ 10-4), but not with blood pressure or lipids. By stepwise multivariate regression analysis, free and total T (both positive) and bio T (negative) were correlated with SHBG in all 4 assays (each P < 10 -15, R2 ≥ 0.481). In addition, TAF and BMI were negatively associated with SHBG (P ≤ 0.0066) in 2 SHBG assays, and estrone and IL-8 with SHBG weakly (P ≤ 0.035) in one SHBG assay each. When nonsignificant cytokines were excluded, SHBG was jointly associated with AVF, total T and HDL (P < 10-9, R2 = 0.358). Conclusion According to MS, three metabolic factors, T, AVF and HDL, together explain more than one-third of the interindividual variation in SHBG levels. We speculate that these measures reflect insulin action.
AB - Objective Sex-hormone binding globulin (SHBG) concentrations across the adult female lifespan are not well defined. To address this knowledge gap, SHBG was quantified by both immunological and criterion methods, viz, mass spectrometry (MS). Methods Setting: Center for Translational Science Activities (CTSA). Participants: Healthy nonpregnant women (N = 120) ages 21 to 79 years. Outcomes: SHBG, testosterone (T), estradiol (E2) and estrone (E 1) each determined by MS. Uni- and multivariate regression of SHBG concentrations on age, body mass index (BMI), total and visceral abdominal fat (TAF, AVF), albumin, glucose, insulin, sex steroids, selected cytokines, blood pressure, and lipids. Results By univariate regression, MS-estimated SHBG correlated negatively with BMI, TAF, AVF, insulin, free T and bioavailable T (bio T) (each P ≤ 10-4), but not with blood pressure or lipids. By stepwise multivariate regression analysis, free and total T (both positive) and bio T (negative) were correlated with SHBG in all 4 assays (each P < 10 -15, R2 ≥ 0.481). In addition, TAF and BMI were negatively associated with SHBG (P ≤ 0.0066) in 2 SHBG assays, and estrone and IL-8 with SHBG weakly (P ≤ 0.035) in one SHBG assay each. When nonsignificant cytokines were excluded, SHBG was jointly associated with AVF, total T and HDL (P < 10-9, R2 = 0.358). Conclusion According to MS, three metabolic factors, T, AVF and HDL, together explain more than one-third of the interindividual variation in SHBG levels. We speculate that these measures reflect insulin action.
KW - Body composition
KW - Female
KW - Insulin
KW - Obesity
KW - Sex steroid
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U2 - 10.1016/j.metabol.2014.03.010
DO - 10.1016/j.metabol.2014.03.010
M3 - Article
C2 - 24746136
AN - SCOPUS:84901197845
SN - 0026-0495
VL - 63
SP - 783
EP - 792
JO - Metabolism: Clinical and Experimental
JF - Metabolism: Clinical and Experimental
IS - 6
ER -