Immunolocalization of calcitriol receptor, plasma membrane calcium pump and casbindin-D(28k) in the cornea and ciliary body of the rat eye

J. A. Johnson, Joseph Peter Grande, P. C. Roche, R. J. Campbell, Rajiv Kumar

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Epitopes of the calcitriol receptor, the ATP-dependent plasma membrane calcium pump (PMCA) and the 28-kD vitamin-D-dependent Ca-binding protein (calbindin-D(28k)) were detected in sections of the albino rat eye using light microscopy and immunohistochemistry of paraffin-embedded tissues. Calcitriol receptor and calbindin-D(28k) epitopes were detected in both the inner and outer layers of the ciliary body epithelium. PMCA was present in both epithelial cell layers of the ciliary body but was most prominent in the inner layer. Corneal epithelium and endothelium also contained epitopes for calcitriol receptor, PMCA and calbindin-D(28k). These Ca-regulatory proteins may play a role in the cellular physiology of the albino rat eye by maintaining appropriate intracellular and aqueous humor Ca concentrations.

Original languageEnglish (US)
Pages (from-to)42-47
Number of pages6
JournalOphthalmic Research
Volume27
Issue number1
StatePublished - 1995

Fingerprint

Calbindins
Ciliary Body
Calcitriol Receptors
Cornea
Epitopes
Cell Membrane
Calcium
Corneal Endothelium
Corneal Epithelium
Aqueous Humor
Vitamin D
Paraffin
Microscopy
Carrier Proteins
Epithelium
Adenosine Triphosphate
Epithelial Cells
Immunohistochemistry
Light
Proteins

Keywords

  • Calbindin-D(28k)
  • Calcitriol receptor
  • Ciliary body
  • Cornea
  • Plasma membrane calcium pump
  • Rat

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Immunolocalization of calcitriol receptor, plasma membrane calcium pump and casbindin-D(28k) in the cornea and ciliary body of the rat eye. / Johnson, J. A.; Grande, Joseph Peter; Roche, P. C.; Campbell, R. J.; Kumar, Rajiv.

In: Ophthalmic Research, Vol. 27, No. 1, 1995, p. 42-47.

Research output: Contribution to journalArticle

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AU - Kumar, Rajiv

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