Immunohistologic labeling as an indicator of liver allograft rejection

J. D. Perkins, R. H. Wiesner, P. M. Banks, Nicholas F La Russo, J. Ludwig, R. A. Krom

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Abstract

Monoclonal antibodies were used to identify T-helper cells (T(H)) and T-suppressor/cytotoxic cells (T(S/C)) in biopsy specimens obtained 7, 21, 90, 180, and 365 days postoperatively, and during episodes of graft dysfunction, from 34 consecutive liver transplant patients treated with cyclosporine and steroids. Rejection was diagnosed by the presence of appropriate laboratory and light microscopic findings and at least 8 weeks of follow-up to exclude other causes of graft dysfunction. Four immunohistologic patterns were seen - no labeled cells (No), only lobular T(S/C), only portal T(H), and a portal mixture of T(H) and T(S/C) (mix). Of 36 specimens with the No or only lobular T(S/C) pattern, 29 were not associated with rejection. Of the 39 specimens with the portal T(H) or portal Mix pattern, 33 were associated with a rejection episode. In addition, in nine specimens from patients with no biochemical or routine histologic evidence of rejection, the presence of portal T(H) or a portal mix indicated immunologic rejection 5 days to 5 weeks before biochemical and routine histologic evidence of it was manifested. Immunohistologic labeling appears to be an early indicator of liver allograft rejection.

Original languageEnglish (US)
Pages (from-to)105-108
Number of pages4
JournalTransplantation
Volume43
Issue number1
StatePublished - 1987

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Helper-Inducer T-Lymphocytes
Allografts
Liver
Transplants
Cyclosporine
Steroids
Monoclonal Antibodies
Biopsy
Light

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Perkins, J. D., Wiesner, R. H., Banks, P. M., La Russo, N. F., Ludwig, J., & Krom, R. A. (1987). Immunohistologic labeling as an indicator of liver allograft rejection. Transplantation, 43(1), 105-108.

Immunohistologic labeling as an indicator of liver allograft rejection. / Perkins, J. D.; Wiesner, R. H.; Banks, P. M.; La Russo, Nicholas F; Ludwig, J.; Krom, R. A.

In: Transplantation, Vol. 43, No. 1, 1987, p. 105-108.

Research output: Contribution to journalArticle

Perkins, JD, Wiesner, RH, Banks, PM, La Russo, NF, Ludwig, J & Krom, RA 1987, 'Immunohistologic labeling as an indicator of liver allograft rejection', Transplantation, vol. 43, no. 1, pp. 105-108.
Perkins JD, Wiesner RH, Banks PM, La Russo NF, Ludwig J, Krom RA. Immunohistologic labeling as an indicator of liver allograft rejection. Transplantation. 1987;43(1):105-108.
Perkins, J. D. ; Wiesner, R. H. ; Banks, P. M. ; La Russo, Nicholas F ; Ludwig, J. ; Krom, R. A. / Immunohistologic labeling as an indicator of liver allograft rejection. In: Transplantation. 1987 ; Vol. 43, No. 1. pp. 105-108.
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AU - Krom, R. A.

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