Immunohistologic evaluation of invasion-associated proteases in breast carcinoma.

Daniel W Visscher, F. Sarkar, P. LoRusso, W. Sakr, S. Ottosen, S. Wykes, J. D. Crissman

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Abstract

Immunostaining of two invasion-associated proteolytic enzymes, cathepsin D (CD) and urokinase-type plasminogen activator (uPA), was assessed in cryostat sections of 86 stage-heterogeneous breast carcinomas using monoclonal antibodies. Most tumors displayed a focal and/or heterogeneous staining pattern. Overall, staining was more frequent in host-derived stromal and inflammatory cells (uPA 54%, CD 89%) than neoplastic epithelium per se (uPA 24%, CD 70%). Intense (i.e., 2+) stromal, but not neoplastic, CD was significantly correlated with nodal or systematic metastases (node negative--10% versus node positive/systemic--33%, p = 0.04). Further, cumulative staining of more than one enzyme (CD + uPA) or more than one tumor component (stroma + epithelium) correlated with metastatic disease (no metastases--35% versus metastatic--72%, p = 0.005). Neither stromal nor epithelial CD alone was significantly correlated with short-term recurrence free survival, however additive CD staining (i.e., stromal + epithelial) was strongly predictive, overall (both + -75% recurred versus both weak/negative--16% recurred, p = 0.0004) and in node positive patients (p = 0.02). We conclude that (a) enzymes putatively mediating extracellular matrix dissolution may be derived from multiple sources and (b) the metastatic capacity and/or clinical aggressiveness of breast carcinomas may reflect overall proteolytic enzyme expression, suggesting that cooperative enzyme interaction may be required for invasive growth and/or metastasis.

Original languageEnglish (US)
Pages (from-to)302-306
Number of pages5
JournalModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
Volume6
Issue number3
StatePublished - May 1993
Externally publishedYes

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Cathepsin D
Peptide Hydrolases
Breast Neoplasms
Urokinase-Type Plasminogen Activator
Staining and Labeling
Neoplasm Metastasis
Enzymes
Epithelium
Stromal Cells
Extracellular Matrix
Neoplasms
Monoclonal Antibodies
Recurrence
Survival
Growth

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Immunohistologic evaluation of invasion-associated proteases in breast carcinoma. / Visscher, Daniel W; Sarkar, F.; LoRusso, P.; Sakr, W.; Ottosen, S.; Wykes, S.; Crissman, J. D.

In: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 6, No. 3, 05.1993, p. 302-306.

Research output: Contribution to journalArticle

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abstract = "Immunostaining of two invasion-associated proteolytic enzymes, cathepsin D (CD) and urokinase-type plasminogen activator (uPA), was assessed in cryostat sections of 86 stage-heterogeneous breast carcinomas using monoclonal antibodies. Most tumors displayed a focal and/or heterogeneous staining pattern. Overall, staining was more frequent in host-derived stromal and inflammatory cells (uPA 54{\%}, CD 89{\%}) than neoplastic epithelium per se (uPA 24{\%}, CD 70{\%}). Intense (i.e., 2+) stromal, but not neoplastic, CD was significantly correlated with nodal or systematic metastases (node negative--10{\%} versus node positive/systemic--33{\%}, p = 0.04). Further, cumulative staining of more than one enzyme (CD + uPA) or more than one tumor component (stroma + epithelium) correlated with metastatic disease (no metastases--35{\%} versus metastatic--72{\%}, p = 0.005). Neither stromal nor epithelial CD alone was significantly correlated with short-term recurrence free survival, however additive CD staining (i.e., stromal + epithelial) was strongly predictive, overall (both + -75{\%} recurred versus both weak/negative--16{\%} recurred, p = 0.0004) and in node positive patients (p = 0.02). We conclude that (a) enzymes putatively mediating extracellular matrix dissolution may be derived from multiple sources and (b) the metastatic capacity and/or clinical aggressiveness of breast carcinomas may reflect overall proteolytic enzyme expression, suggesting that cooperative enzyme interaction may be required for invasive growth and/or metastasis.",
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AU - Sarkar, F.

AU - LoRusso, P.

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AU - Ottosen, S.

AU - Wykes, S.

AU - Crissman, J. D.

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