TY - JOUR
T1 - Immunohistologic analysis of invasive phenotype in breast carcinoma
T2 - A clinicopathologic study
AU - Visscher, D. W.
AU - Sarkar, F. H.
AU - Sakr, W.
AU - Crissman, J.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1993
Y1 - 1993
N2 - Acetone-fixed, cryostat sections of 81 snap-frozen invasive breast carcinomas were immunostained with monoclonal antibodies to Cathepsin D (CD), a protease believed to mediate extracellular matrix dissolution, and Type IV collagen, a constituent of basal lamina (BL). Most cases (48/81, 53 %) exhibited focal, patchy BL distribution (1 +) around tumor cell nests, although subsets with diffuse continuous (2 +) peritumoral sheets (15/81,19 %) or nearcomplete absence (0 +, 23/81, 28 %) were also observed. Elaboration of BL was correlated with favorable morphologic differentiation (0+ BL - 57 % poorly differentiated vs. 2+ BL - 13 % poorly differentiated, p = .01), absence of nodal or systemic metastases (0+ BL - 78 % metastatic vs. 2+ BL - 40 % metastatic, p = .02), and improved disease-free survival (0+ BL - 63 % recurred vs. 2+ BL -s 20 % recurred, p = .05). In addition, neoplastic cells expressed CD more frequently in tumors which lacked detectable BL synthesis (0+ BL - 91 % CD+ vs. 2+ BL - 57% CD+, P = .03). The observed relationships between morphologic growth pattern, BL synthesis and CD expression imply conventional grading in large parts reflects activity or extent of host tissue invasion by a given neoplasm. Widespread but heterogeneous distribution of BL in breast tumors also suggests partial equilibrium between neoplastic and host tissues in most cases.
AB - Acetone-fixed, cryostat sections of 81 snap-frozen invasive breast carcinomas were immunostained with monoclonal antibodies to Cathepsin D (CD), a protease believed to mediate extracellular matrix dissolution, and Type IV collagen, a constituent of basal lamina (BL). Most cases (48/81, 53 %) exhibited focal, patchy BL distribution (1 +) around tumor cell nests, although subsets with diffuse continuous (2 +) peritumoral sheets (15/81,19 %) or nearcomplete absence (0 +, 23/81, 28 %) were also observed. Elaboration of BL was correlated with favorable morphologic differentiation (0+ BL - 57 % poorly differentiated vs. 2+ BL - 13 % poorly differentiated, p = .01), absence of nodal or systemic metastases (0+ BL - 78 % metastatic vs. 2+ BL - 40 % metastatic, p = .02), and improved disease-free survival (0+ BL - 63 % recurred vs. 2+ BL -s 20 % recurred, p = .05). In addition, neoplastic cells expressed CD more frequently in tumors which lacked detectable BL synthesis (0+ BL - 91 % CD+ vs. 2+ BL - 57% CD+, P = .03). The observed relationships between morphologic growth pattern, BL synthesis and CD expression imply conventional grading in large parts reflects activity or extent of host tissue invasion by a given neoplasm. Widespread but heterogeneous distribution of BL in breast tumors also suggests partial equilibrium between neoplastic and host tissues in most cases.
KW - Basal lamina
KW - Breast carcinoma
KW - Cathepsin D
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U2 - 10.1016/S0344-0338(11)81096-4
DO - 10.1016/S0344-0338(11)81096-4
M3 - Article
C2 - 8302708
AN - SCOPUS:0027377044
SN - 0344-0338
VL - 189
SP - 867
EP - 872
JO - Pathology Research and Practice
JF - Pathology Research and Practice
IS - 8
ER -