Immunohistochemical testing of conventional adenomas for loss of expression of mismatch repair proteins in Lynch syndrome mutation carriers: A case series from the Australasian site of the colon cancer family registry

Michael D. Walsh, Daniel D. Buchanan, Sally Ann Pearson, Mark Clendenning, Mark A. Jenkins, Aung Ko Win, Rhiannon J. Walters, Kevin J. Spring, Belinda Nagler, Erika Pavluk, Sven T. Arnold, Jack Goldblatt, Jill George, Graeme K. Suthers, Kerry Phillips, John L. Hopper, Jeremy R. Jass, John A. Baron, Dennis J. Ahnen, Stephen N ThibodeauNoralane Morey Lindor, Susan Parry, Neal I. Walker, Christophe Rosty, Joanne P. Young

Research output: Contribution to journalArticle

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Abstract

Debate continues as to the usefulness of assessing adenomas for loss of mismatch repair protein expression to identify individuals with suspected Lynch syndrome. We tested 109 polyps from 69 proven mutation carriers (35 females and 34 males) belonging to 49 Lynch syndrome families. All polyps were tested by immunohistochemistry for four mismatch repair proteins MLH1, MSH2, MSH6 and PMS2. Detailed pathology review was performed by specialist gastrointestinal pathologists. The majority of polyps (86%) were conventional adenomas (n=94), with 65 tubular and 28 tubulovillous adenomas and a single villous adenoma. The remaining 15 lesions (14%) were serrated polyps. Overall, loss of mismatch repair expression was noted for 78/109 (72%) of polyps. Loss of mismatch repair expression was seen in 74 of 94 (79%) conventional adenomas, and 4 of 15 (27%) serrated polyps from mismatch repair gene mutation carriers. In all instances, loss of expression was consistent with the underlying germline mutation. Mismatch repair protein expression was lost in 27 of 29 adenomas with a villous component compared with 47 of 65 adenomas without this feature (93 vs 73%; P=0.028). A strong trend was observed for high-grade dysplasia. Mismatch repair deficiency was observed in 12 of 12 conventional adenomas with high-grade dysplasia compared with 60 of 79 with low-grade dysplasia (100 vs 76%; P=0.065). We were unable to demonstrate a significant association between conventional adenoma size or site and mismatch repair deficiency. All (4/4 or 100%) of the serrated polyps demonstrating mismatch repair deficiency were traditional serrated adenomas from a single family. Diagnostic testing of adenomas in suspected Lynch syndrome families is a useful alternative in cases where cancers are unavailable. The overwhelming majority of conventional adenomas from mutation carriers show loss of mismatch repair protein expression concordant with the underlying germline mutation.

Original languageEnglish (US)
Pages (from-to)722-730
Number of pages9
JournalModern Pathology
Volume25
Issue number5
DOIs
StatePublished - May 2012

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Hereditary Nonpolyposis Colorectal Neoplasms
DNA Mismatch Repair
Adenoma
Colonic Neoplasms
Registries
Polyps
Mutation
Proteins
Germ-Line Mutation
Villous Adenoma
Immunohistochemistry

Keywords

  • adenomas
  • Lynch syndrome

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Immunohistochemical testing of conventional adenomas for loss of expression of mismatch repair proteins in Lynch syndrome mutation carriers : A case series from the Australasian site of the colon cancer family registry. / Walsh, Michael D.; Buchanan, Daniel D.; Pearson, Sally Ann; Clendenning, Mark; Jenkins, Mark A.; Win, Aung Ko; Walters, Rhiannon J.; Spring, Kevin J.; Nagler, Belinda; Pavluk, Erika; Arnold, Sven T.; Goldblatt, Jack; George, Jill; Suthers, Graeme K.; Phillips, Kerry; Hopper, John L.; Jass, Jeremy R.; Baron, John A.; Ahnen, Dennis J.; Thibodeau, Stephen N; Lindor, Noralane Morey; Parry, Susan; Walker, Neal I.; Rosty, Christophe; Young, Joanne P.

In: Modern Pathology, Vol. 25, No. 5, 05.2012, p. 722-730.

Research output: Contribution to journalArticle

Walsh, MD, Buchanan, DD, Pearson, SA, Clendenning, M, Jenkins, MA, Win, AK, Walters, RJ, Spring, KJ, Nagler, B, Pavluk, E, Arnold, ST, Goldblatt, J, George, J, Suthers, GK, Phillips, K, Hopper, JL, Jass, JR, Baron, JA, Ahnen, DJ, Thibodeau, SN, Lindor, NM, Parry, S, Walker, NI, Rosty, C & Young, JP 2012, 'Immunohistochemical testing of conventional adenomas for loss of expression of mismatch repair proteins in Lynch syndrome mutation carriers: A case series from the Australasian site of the colon cancer family registry', Modern Pathology, vol. 25, no. 5, pp. 722-730. https://doi.org/10.1038/modpathol.2011.209
Walsh, Michael D. ; Buchanan, Daniel D. ; Pearson, Sally Ann ; Clendenning, Mark ; Jenkins, Mark A. ; Win, Aung Ko ; Walters, Rhiannon J. ; Spring, Kevin J. ; Nagler, Belinda ; Pavluk, Erika ; Arnold, Sven T. ; Goldblatt, Jack ; George, Jill ; Suthers, Graeme K. ; Phillips, Kerry ; Hopper, John L. ; Jass, Jeremy R. ; Baron, John A. ; Ahnen, Dennis J. ; Thibodeau, Stephen N ; Lindor, Noralane Morey ; Parry, Susan ; Walker, Neal I. ; Rosty, Christophe ; Young, Joanne P. / Immunohistochemical testing of conventional adenomas for loss of expression of mismatch repair proteins in Lynch syndrome mutation carriers : A case series from the Australasian site of the colon cancer family registry. In: Modern Pathology. 2012 ; Vol. 25, No. 5. pp. 722-730.
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AU - Walsh, Michael D.

AU - Buchanan, Daniel D.

AU - Pearson, Sally Ann

AU - Clendenning, Mark

AU - Jenkins, Mark A.

AU - Win, Aung Ko

AU - Walters, Rhiannon J.

AU - Spring, Kevin J.

AU - Nagler, Belinda

AU - Pavluk, Erika

AU - Arnold, Sven T.

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AU - Phillips, Kerry

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AU - Baron, John A.

AU - Ahnen, Dennis J.

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AU - Lindor, Noralane Morey

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N2 - Debate continues as to the usefulness of assessing adenomas for loss of mismatch repair protein expression to identify individuals with suspected Lynch syndrome. We tested 109 polyps from 69 proven mutation carriers (35 females and 34 males) belonging to 49 Lynch syndrome families. All polyps were tested by immunohistochemistry for four mismatch repair proteins MLH1, MSH2, MSH6 and PMS2. Detailed pathology review was performed by specialist gastrointestinal pathologists. The majority of polyps (86%) were conventional adenomas (n=94), with 65 tubular and 28 tubulovillous adenomas and a single villous adenoma. The remaining 15 lesions (14%) were serrated polyps. Overall, loss of mismatch repair expression was noted for 78/109 (72%) of polyps. Loss of mismatch repair expression was seen in 74 of 94 (79%) conventional adenomas, and 4 of 15 (27%) serrated polyps from mismatch repair gene mutation carriers. In all instances, loss of expression was consistent with the underlying germline mutation. Mismatch repair protein expression was lost in 27 of 29 adenomas with a villous component compared with 47 of 65 adenomas without this feature (93 vs 73%; P=0.028). A strong trend was observed for high-grade dysplasia. Mismatch repair deficiency was observed in 12 of 12 conventional adenomas with high-grade dysplasia compared with 60 of 79 with low-grade dysplasia (100 vs 76%; P=0.065). We were unable to demonstrate a significant association between conventional adenoma size or site and mismatch repair deficiency. All (4/4 or 100%) of the serrated polyps demonstrating mismatch repair deficiency were traditional serrated adenomas from a single family. Diagnostic testing of adenomas in suspected Lynch syndrome families is a useful alternative in cases where cancers are unavailable. The overwhelming majority of conventional adenomas from mutation carriers show loss of mismatch repair protein expression concordant with the underlying germline mutation.

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