Immunohistochemical studies of c-kit, transforming growth factor-β, and basic fibroblast growth factor in mast cell disease

Jin Young Baek, Ayalew Tefferi, Animesh Pardanani, Chin Yang Li

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

In 58 patients with mast cell disease (MCD) and three with basophilic leukemia, bone marrow (54 cases) or skin tissue (four cases) was studied immunohistochemically for expression of Kit (c-kit protein), the different isomers of transforming growth factor-β (TGF-β), basic fibroblast growth factor (bFGF), and their respective receptors. Kit was expressed in all cases of MCD but in none of basophilic leukemia. Expression pattern of cytokines and their receptors was variable in systemic MCD with (SMCD-HD) or without (SMCD) associated hematologic disorder. However, type I TGF-β receptor (TGFβ1R) was not expressed in 30% of SMCD-HD patients or in patients with mast cell leukemia, but the remaining cases of MCD showed near uniform expression. The associated hematologic disorders in TGFβ1R-negative cases of SMCD-HD were prognostically less favorable than those associated with TGFβ1R-positive cases of SMCD-HD. The results confirm the diagnostic value of Kit immunohistochemistry in MCD and suggest a biologically relevant heterogeneity in TGFβ1R expression among patients with SMCD-HD.

Original languageEnglish (US)
Pages (from-to)83-90
Number of pages8
JournalLeukemia Research
Volume26
Issue number1
DOIs
StatePublished - Jan 1 2002

Keywords

  • Basophilic leukemia
  • Cytokine
  • Immunohistochemistry
  • Mast cell disease
  • Mastocytosis
  • Stem cell factor

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Immunohistochemical studies of c-kit, transforming growth factor-β, and basic fibroblast growth factor in mast cell disease'. Together they form a unique fingerprint.

  • Cite this