Immunohistochemical localization of the 1,25(OH)2D3 receptor and calbindin D(28k) in human and rat pancreas

J. A. Johnson, J. P. Grande, P. C. Roche, R. Kumar

Research output: Contribution to journalArticle

190 Scopus citations

Abstract

1,25-Dihydroxyvitamin D3 [1,25(OH)2D3] is required for normal glucose- stimulated insulin release from pancreatic β-cells. Biochemical characterization techniques have demonstrated the presence of the 1,25(OH)2D3 receptor (VDR) in homogenates of whole pancreas. Autoradiographic studies using radiolabeled 1,25(OH)2D3 suggest that the VDR is localized to β-cells but are inconclusive. We used immunohistochemical techniques to stain serial sections from both human and rat pancreas with polyclonal antibodies to human VDR, chick calbindin D(28k), insulin, glucagon, and somatostatin. VDR was present in the islet cells and also at low levels in acinar cells of the human and rat pancreas. Calbindin D(28k) was distributed in a manner similar to the VDR in pancreatic islets but was not present in acini. These results show for the first time that VDR and calbindin D(28k) are present in human pancreatic tissue. VDR and calbindin D(28k) are focally distributed throughout pancreatic islet cell types in humans and rats; VDR is also present in the exocrine pancreas. These findings suggest that 1,25(OH)2D3 may influence both endocrine and exocrine pancreatic function.

Original languageEnglish (US)
Pages (from-to)E356-E360
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume267
Issue number3 30-3
DOIs
StatePublished - 1994

Keywords

  • 1,25-dihydroxyvitamin D
  • calbindin
  • calcium binding protein
  • insulin
  • pancreas
  • vitamin D receptor
  • β-cells

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

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