Immunohistochemical localisation of the serotonin 5-HT2B receptor in mouse gut, cardiovascular system, and brain

Doo Sup Choi, Luc Maroteaux

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

We recently reported the cloning of a new member of the serotonin 5-HT2 family, the 5-HT2B receptor. We now report the production and characterisation of a specific antiserum directed against the C-terminal portion of the mouse 5-HT2B receptor. After affinity purification, this polyclonal antibody recognises specifically the mouse 5-HT2B receptor. Immunohistochemical analysis of cryosections from various adult mouse tissues reveals a major 5-HT2B receptor expression in stomach, intestine and pulmonary smooth muscles as well as in myocardium. Furthermore, the antiserum recognises specific areas of the mouse brain, including cerebellar Purkinje cells and their projection areas.

Original languageEnglish (US)
Pages (from-to)45-51
Number of pages7
JournalFEBS Letters
Volume391
Issue number1-2
DOIs
StatePublished - Aug 5 1996

Keywords

  • Antibodies
  • Cerebellum
  • Heart
  • Smooth muscle

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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