Immunohistochemical localisation of the serotonin 5-HT2B receptor in mouse gut, cardiovascular system, and brain

Doo Sup Choi, Luc Maroteaux

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

We recently reported the cloning of a new member of the serotonin 5-HT2 family, the 5-HT2B receptor. We now report the production and characterisation of a specific antiserum directed against the C-terminal portion of the mouse 5-HT2B receptor. After affinity purification, this polyclonal antibody recognises specifically the mouse 5-HT2B receptor. Immunohistochemical analysis of cryosections from various adult mouse tissues reveals a major 5-HT2B receptor expression in stomach, intestine and pulmonary smooth muscles as well as in myocardium. Furthermore, the antiserum recognises specific areas of the mouse brain, including cerebellar Purkinje cells and their projection areas.

Original languageEnglish (US)
Pages (from-to)45-51
Number of pages7
JournalFEBS Letters
Volume391
Issue number1-2
DOIs
StatePublished - Aug 5 1996
Externally publishedYes

Fingerprint

Receptor, Serotonin, 5-HT2B
Cardiovascular system
Cardiovascular System
Brain
Immune Sera
Cloning
Purkinje Cells
Intestines
Purification
Smooth Muscle
Muscle
Organism Cloning
Serotonin
Stomach
Myocardium
Tissue
Lung
Antibodies

Keywords

  • Antibodies
  • Cerebellum
  • Heart
  • Smooth muscle

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Immunohistochemical localisation of the serotonin 5-HT2B receptor in mouse gut, cardiovascular system, and brain. / Choi, Doo Sup; Maroteaux, Luc.

In: FEBS Letters, Vol. 391, No. 1-2, 05.08.1996, p. 45-51.

Research output: Contribution to journalArticle

@article{189e49d393ae470d95a50adf1df39466,
title = "Immunohistochemical localisation of the serotonin 5-HT2B receptor in mouse gut, cardiovascular system, and brain",
abstract = "We recently reported the cloning of a new member of the serotonin 5-HT2 family, the 5-HT2B receptor. We now report the production and characterisation of a specific antiserum directed against the C-terminal portion of the mouse 5-HT2B receptor. After affinity purification, this polyclonal antibody recognises specifically the mouse 5-HT2B receptor. Immunohistochemical analysis of cryosections from various adult mouse tissues reveals a major 5-HT2B receptor expression in stomach, intestine and pulmonary smooth muscles as well as in myocardium. Furthermore, the antiserum recognises specific areas of the mouse brain, including cerebellar Purkinje cells and their projection areas.",
keywords = "Antibodies, Cerebellum, Heart, Smooth muscle",
author = "Choi, {Doo Sup} and Luc Maroteaux",
year = "1996",
month = "8",
day = "5",
doi = "10.1016/0014-5793(96)00695-3",
language = "English (US)",
volume = "391",
pages = "45--51",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Immunohistochemical localisation of the serotonin 5-HT2B receptor in mouse gut, cardiovascular system, and brain

AU - Choi, Doo Sup

AU - Maroteaux, Luc

PY - 1996/8/5

Y1 - 1996/8/5

N2 - We recently reported the cloning of a new member of the serotonin 5-HT2 family, the 5-HT2B receptor. We now report the production and characterisation of a specific antiserum directed against the C-terminal portion of the mouse 5-HT2B receptor. After affinity purification, this polyclonal antibody recognises specifically the mouse 5-HT2B receptor. Immunohistochemical analysis of cryosections from various adult mouse tissues reveals a major 5-HT2B receptor expression in stomach, intestine and pulmonary smooth muscles as well as in myocardium. Furthermore, the antiserum recognises specific areas of the mouse brain, including cerebellar Purkinje cells and their projection areas.

AB - We recently reported the cloning of a new member of the serotonin 5-HT2 family, the 5-HT2B receptor. We now report the production and characterisation of a specific antiserum directed against the C-terminal portion of the mouse 5-HT2B receptor. After affinity purification, this polyclonal antibody recognises specifically the mouse 5-HT2B receptor. Immunohistochemical analysis of cryosections from various adult mouse tissues reveals a major 5-HT2B receptor expression in stomach, intestine and pulmonary smooth muscles as well as in myocardium. Furthermore, the antiserum recognises specific areas of the mouse brain, including cerebellar Purkinje cells and their projection areas.

KW - Antibodies

KW - Cerebellum

KW - Heart

KW - Smooth muscle

UR - http://www.scopus.com/inward/record.url?scp=0030570807&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030570807&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(96)00695-3

DO - 10.1016/0014-5793(96)00695-3

M3 - Article

C2 - 8706927

AN - SCOPUS:0030570807

VL - 391

SP - 45

EP - 51

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-2

ER -