@article{189e49d393ae470d95a50adf1df39466,
title = "Immunohistochemical localisation of the serotonin 5-HT2B receptor in mouse gut, cardiovascular system, and brain",
abstract = "We recently reported the cloning of a new member of the serotonin 5-HT2 family, the 5-HT2B receptor. We now report the production and characterisation of a specific antiserum directed against the C-terminal portion of the mouse 5-HT2B receptor. After affinity purification, this polyclonal antibody recognises specifically the mouse 5-HT2B receptor. Immunohistochemical analysis of cryosections from various adult mouse tissues reveals a major 5-HT2B receptor expression in stomach, intestine and pulmonary smooth muscles as well as in myocardium. Furthermore, the antiserum recognises specific areas of the mouse brain, including cerebellar Purkinje cells and their projection areas.",
keywords = "Antibodies, Cerebellum, Heart, Smooth muscle",
author = "Choi, {Doo Sup} and Luc Maroteaux",
note = "Funding Information: AcknowledgemenWtse: wish to acknowledgPe. Hickel and T. Ding for excellentte chnicaals sistanceM, . Acker and B. Hellerf or helpi n tissuec ultureo f cells,A . Stauba ndP. Eberlingfo r peptidesy nthesis, G. Duval for rabbiti njectionas ndc are,L . Tora for antiserumpu r-ificationa ndD. Decimof or immunocytochemisatdrvyi ce,B . Boulay andJ .-M. Lafontainfeo r helpi n preparintgh ea rtworkW. e thankD r. S.J. Ward for criticalr eadingo f them anuscripatn dD r. J.-M. Launay for helpfuld iscussionsT.h is work has beens upportebdy fundsf rom the CentreN ationald e la RechercheS cientifiquet,h e InstitutN a-tionald e la Sant6e t de la RechercheM rdical,t heC entreH ospitalier UniversitairRer gional,a ndby grantsfr omthe EuropeanE conomic Communityfr, omthe Minist~red e l'EnseigemeSnut prrieuer t de la Recherchef,r om the Fondationp our la RecherchMe rdicale,a nd from the Associatiopno urla Recherchec ontrele Cancer# 6800.",
year = "1996",
month = aug,
day = "5",
doi = "10.1016/0014-5793(96)00695-3",
language = "English (US)",
volume = "391",
pages = "45--51",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1-2",
}