Immunohistochemical detection of bcl-2 protein in adenocarcinoma and Non-Neoplastic cellular compartments of the lung

Sudha K. Rao, Murli Krishna, Bruce A. Woda, Lou Savas, Armando E. Fraire

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The expression of bcl-2 seems to influence the biologic activity of a variety of neoplasms. In this study, we examined bcl-2 protein expression in 47 surgically resected adenocarcinomas of the lung to determine its expression in the neoplastic and adjacent non-neoplastic cells. Seven squamous cell carcinomas, six small cell carcinomas, five non-neoplastic lung lesions, and five normal lungs were also studied. The bcl-2 protein was detected after antigen retrieval with bcl-2 monoclonal antibody using archival formalin-fixed, paraffin-embedded tissue material. Fifty-one of 52 cases had bronchioles that could be evaluated and all expressed bcl-2, and of these, 12 showed basal immunostaining, 17 showed luminal immunostaining, and 22 showed immunostaining in both cellular compartments. Thirty of the 47 adenocarcinomas expressed bcl-2, whereas four of seven squamous cell carcinomas and all six small cell carcinomas were bcl-2 positive, bcl-2 immunostaining was correlated with a survival using the Fisher exact test. There were no statistically significant differences in survival between patients with bcl-2-positive and bcl-2-negative adenocarcinomas (P = 0.332). Similar results were obtained using the Kaplan-Mier product limit survival analysis method (P = 0.356). Our study also showed that expression of bcl-2 is common in pulmonary adenocarcinomas and that bcl-2 expression may be found in the basal and the luminal surfaces of normal bronchioles.

Original languageEnglish (US)
Pages (from-to)555-559
Number of pages5
JournalModern Pathology
Volume9
Issue number5
StatePublished - May 1996
Externally publishedYes

Fingerprint

Bronchioles
Small Cell Carcinoma
Squamous Cell Carcinoma
Adenocarcinoma
Lung
Survival
Survival Analysis
Paraffin
Formaldehyde
Proteins
Monoclonal Antibodies
Antigens
Neoplasms
Adenocarcinoma of lung

Keywords

  • Adenocarcinoma
  • bcl-2 protein
  • Bronchioles
  • Immunostaining
  • Lung

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Rao, S. K., Krishna, M., Woda, B. A., Savas, L., & Fraire, A. E. (1996). Immunohistochemical detection of bcl-2 protein in adenocarcinoma and Non-Neoplastic cellular compartments of the lung. Modern Pathology, 9(5), 555-559.

Immunohistochemical detection of bcl-2 protein in adenocarcinoma and Non-Neoplastic cellular compartments of the lung. / Rao, Sudha K.; Krishna, Murli; Woda, Bruce A.; Savas, Lou; Fraire, Armando E.

In: Modern Pathology, Vol. 9, No. 5, 05.1996, p. 555-559.

Research output: Contribution to journalArticle

Rao, SK, Krishna, M, Woda, BA, Savas, L & Fraire, AE 1996, 'Immunohistochemical detection of bcl-2 protein in adenocarcinoma and Non-Neoplastic cellular compartments of the lung', Modern Pathology, vol. 9, no. 5, pp. 555-559.
Rao, Sudha K. ; Krishna, Murli ; Woda, Bruce A. ; Savas, Lou ; Fraire, Armando E. / Immunohistochemical detection of bcl-2 protein in adenocarcinoma and Non-Neoplastic cellular compartments of the lung. In: Modern Pathology. 1996 ; Vol. 9, No. 5. pp. 555-559.
@article{dd6e785134334f67af14d45f670660cf,
title = "Immunohistochemical detection of bcl-2 protein in adenocarcinoma and Non-Neoplastic cellular compartments of the lung",
abstract = "The expression of bcl-2 seems to influence the biologic activity of a variety of neoplasms. In this study, we examined bcl-2 protein expression in 47 surgically resected adenocarcinomas of the lung to determine its expression in the neoplastic and adjacent non-neoplastic cells. Seven squamous cell carcinomas, six small cell carcinomas, five non-neoplastic lung lesions, and five normal lungs were also studied. The bcl-2 protein was detected after antigen retrieval with bcl-2 monoclonal antibody using archival formalin-fixed, paraffin-embedded tissue material. Fifty-one of 52 cases had bronchioles that could be evaluated and all expressed bcl-2, and of these, 12 showed basal immunostaining, 17 showed luminal immunostaining, and 22 showed immunostaining in both cellular compartments. Thirty of the 47 adenocarcinomas expressed bcl-2, whereas four of seven squamous cell carcinomas and all six small cell carcinomas were bcl-2 positive, bcl-2 immunostaining was correlated with a survival using the Fisher exact test. There were no statistically significant differences in survival between patients with bcl-2-positive and bcl-2-negative adenocarcinomas (P = 0.332). Similar results were obtained using the Kaplan-Mier product limit survival analysis method (P = 0.356). Our study also showed that expression of bcl-2 is common in pulmonary adenocarcinomas and that bcl-2 expression may be found in the basal and the luminal surfaces of normal bronchioles.",
keywords = "Adenocarcinoma, bcl-2 protein, Bronchioles, Immunostaining, Lung",
author = "Rao, {Sudha K.} and Murli Krishna and Woda, {Bruce A.} and Lou Savas and Fraire, {Armando E.}",
year = "1996",
month = "5",
language = "English (US)",
volume = "9",
pages = "555--559",
journal = "Modern Pathology",
issn = "0893-3952",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Immunohistochemical detection of bcl-2 protein in adenocarcinoma and Non-Neoplastic cellular compartments of the lung

AU - Rao, Sudha K.

AU - Krishna, Murli

AU - Woda, Bruce A.

AU - Savas, Lou

AU - Fraire, Armando E.

PY - 1996/5

Y1 - 1996/5

N2 - The expression of bcl-2 seems to influence the biologic activity of a variety of neoplasms. In this study, we examined bcl-2 protein expression in 47 surgically resected adenocarcinomas of the lung to determine its expression in the neoplastic and adjacent non-neoplastic cells. Seven squamous cell carcinomas, six small cell carcinomas, five non-neoplastic lung lesions, and five normal lungs were also studied. The bcl-2 protein was detected after antigen retrieval with bcl-2 monoclonal antibody using archival formalin-fixed, paraffin-embedded tissue material. Fifty-one of 52 cases had bronchioles that could be evaluated and all expressed bcl-2, and of these, 12 showed basal immunostaining, 17 showed luminal immunostaining, and 22 showed immunostaining in both cellular compartments. Thirty of the 47 adenocarcinomas expressed bcl-2, whereas four of seven squamous cell carcinomas and all six small cell carcinomas were bcl-2 positive, bcl-2 immunostaining was correlated with a survival using the Fisher exact test. There were no statistically significant differences in survival between patients with bcl-2-positive and bcl-2-negative adenocarcinomas (P = 0.332). Similar results were obtained using the Kaplan-Mier product limit survival analysis method (P = 0.356). Our study also showed that expression of bcl-2 is common in pulmonary adenocarcinomas and that bcl-2 expression may be found in the basal and the luminal surfaces of normal bronchioles.

AB - The expression of bcl-2 seems to influence the biologic activity of a variety of neoplasms. In this study, we examined bcl-2 protein expression in 47 surgically resected adenocarcinomas of the lung to determine its expression in the neoplastic and adjacent non-neoplastic cells. Seven squamous cell carcinomas, six small cell carcinomas, five non-neoplastic lung lesions, and five normal lungs were also studied. The bcl-2 protein was detected after antigen retrieval with bcl-2 monoclonal antibody using archival formalin-fixed, paraffin-embedded tissue material. Fifty-one of 52 cases had bronchioles that could be evaluated and all expressed bcl-2, and of these, 12 showed basal immunostaining, 17 showed luminal immunostaining, and 22 showed immunostaining in both cellular compartments. Thirty of the 47 adenocarcinomas expressed bcl-2, whereas four of seven squamous cell carcinomas and all six small cell carcinomas were bcl-2 positive, bcl-2 immunostaining was correlated with a survival using the Fisher exact test. There were no statistically significant differences in survival between patients with bcl-2-positive and bcl-2-negative adenocarcinomas (P = 0.332). Similar results were obtained using the Kaplan-Mier product limit survival analysis method (P = 0.356). Our study also showed that expression of bcl-2 is common in pulmonary adenocarcinomas and that bcl-2 expression may be found in the basal and the luminal surfaces of normal bronchioles.

KW - Adenocarcinoma

KW - bcl-2 protein

KW - Bronchioles

KW - Immunostaining

KW - Lung

UR - http://www.scopus.com/inward/record.url?scp=0029893789&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029893789&partnerID=8YFLogxK

M3 - Article

C2 - 8733771

AN - SCOPUS:0029893789

VL - 9

SP - 555

EP - 559

JO - Modern Pathology

JF - Modern Pathology

SN - 0893-3952

IS - 5

ER -