Immunohistochemical detection and localization of a 72-kilodalton heat shock protein in autoimmune thyroid disease

A. E. Heufelder, J. R. Goellner, B. E. Wenzel, R. S. Bahn

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Recently described immunological functions for heat shock proteins (HSPs) and our previous demonstration of site-selective HSP-72 expression in cultured fibroblasts derived from extrathyroidal manifestations of Graves' disease (GD) prompted us to determine whether expression of the inducible 72- kilodalton HSP can be detected in human thyroid tissues. Immunohistochemistry was performed on formalin-fixed paraffin-embedded thyroid tissue specimens from patients with GD, Hashimoto's thyroiditis (HD), and multinodular goiter (MNG) as well as on normal thyroid tissue. A mouse monoclonal anti-HSP-72 antibody and an ultrasensitive avidin-biotin-peroxidase complex detection system were used for these studies. Striking differences in HSP-72 immunoreactivity were detected both between tissues from GD and HD compared with MNG and normal thyroid and between GD thyroid glands treated preoperatively with antithyroid medication and untreated GD glands. Strong HSP-72 reactivity in GD and HD tissues was detected in thyroid follicles as well lymphocytic infiltrates. No HSP-72 reactivity was detected in MNG or normal thyroid tissue. HSP-72 immunoreactivity was markedly reduced in GD glands that received preoperative antithyroid drug treatment. In conclusion, high levels of HSP-72 expression in autoimmune thyroid disease may reflect a state of chronic cellular stress, but could also represent an immunomodulatory factor of relevance in the autoimmune process in GD.

Original languageEnglish (US)
Pages (from-to)724-731
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Volume74
Issue number4
DOIs
StatePublished - 1992

Fingerprint

HSP72 Heat-Shock Proteins
Graves Disease
Thyroid Diseases
Heat-Shock Proteins
Autoimmune Diseases
Thyroid Gland
Tissue
Goiter
Antithyroid Agents
Hashimoto Disease
Drug therapy
Avidin
Biotin
Paraffin
Fibroblasts
Formaldehyde
Peroxidase
Immunohistochemistry
Demonstrations

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Immunohistochemical detection and localization of a 72-kilodalton heat shock protein in autoimmune thyroid disease. / Heufelder, A. E.; Goellner, J. R.; Wenzel, B. E.; Bahn, R. S.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 74, No. 4, 1992, p. 724-731.

Research output: Contribution to journalArticle

Heufelder, A. E. ; Goellner, J. R. ; Wenzel, B. E. ; Bahn, R. S. / Immunohistochemical detection and localization of a 72-kilodalton heat shock protein in autoimmune thyroid disease. In: Journal of Clinical Endocrinology and Metabolism. 1992 ; Vol. 74, No. 4. pp. 724-731.
@article{11d292946889478faea556fe80831b88,
title = "Immunohistochemical detection and localization of a 72-kilodalton heat shock protein in autoimmune thyroid disease",
abstract = "Recently described immunological functions for heat shock proteins (HSPs) and our previous demonstration of site-selective HSP-72 expression in cultured fibroblasts derived from extrathyroidal manifestations of Graves' disease (GD) prompted us to determine whether expression of the inducible 72- kilodalton HSP can be detected in human thyroid tissues. Immunohistochemistry was performed on formalin-fixed paraffin-embedded thyroid tissue specimens from patients with GD, Hashimoto's thyroiditis (HD), and multinodular goiter (MNG) as well as on normal thyroid tissue. A mouse monoclonal anti-HSP-72 antibody and an ultrasensitive avidin-biotin-peroxidase complex detection system were used for these studies. Striking differences in HSP-72 immunoreactivity were detected both between tissues from GD and HD compared with MNG and normal thyroid and between GD thyroid glands treated preoperatively with antithyroid medication and untreated GD glands. Strong HSP-72 reactivity in GD and HD tissues was detected in thyroid follicles as well lymphocytic infiltrates. No HSP-72 reactivity was detected in MNG or normal thyroid tissue. HSP-72 immunoreactivity was markedly reduced in GD glands that received preoperative antithyroid drug treatment. In conclusion, high levels of HSP-72 expression in autoimmune thyroid disease may reflect a state of chronic cellular stress, but could also represent an immunomodulatory factor of relevance in the autoimmune process in GD.",
author = "Heufelder, {A. E.} and Goellner, {J. R.} and Wenzel, {B. E.} and Bahn, {R. S.}",
year = "1992",
doi = "10.1210/jc.74.4.724",
language = "English (US)",
volume = "74",
pages = "724--731",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "The Endocrine Society",
number = "4",

}

TY - JOUR

T1 - Immunohistochemical detection and localization of a 72-kilodalton heat shock protein in autoimmune thyroid disease

AU - Heufelder, A. E.

AU - Goellner, J. R.

AU - Wenzel, B. E.

AU - Bahn, R. S.

PY - 1992

Y1 - 1992

N2 - Recently described immunological functions for heat shock proteins (HSPs) and our previous demonstration of site-selective HSP-72 expression in cultured fibroblasts derived from extrathyroidal manifestations of Graves' disease (GD) prompted us to determine whether expression of the inducible 72- kilodalton HSP can be detected in human thyroid tissues. Immunohistochemistry was performed on formalin-fixed paraffin-embedded thyroid tissue specimens from patients with GD, Hashimoto's thyroiditis (HD), and multinodular goiter (MNG) as well as on normal thyroid tissue. A mouse monoclonal anti-HSP-72 antibody and an ultrasensitive avidin-biotin-peroxidase complex detection system were used for these studies. Striking differences in HSP-72 immunoreactivity were detected both between tissues from GD and HD compared with MNG and normal thyroid and between GD thyroid glands treated preoperatively with antithyroid medication and untreated GD glands. Strong HSP-72 reactivity in GD and HD tissues was detected in thyroid follicles as well lymphocytic infiltrates. No HSP-72 reactivity was detected in MNG or normal thyroid tissue. HSP-72 immunoreactivity was markedly reduced in GD glands that received preoperative antithyroid drug treatment. In conclusion, high levels of HSP-72 expression in autoimmune thyroid disease may reflect a state of chronic cellular stress, but could also represent an immunomodulatory factor of relevance in the autoimmune process in GD.

AB - Recently described immunological functions for heat shock proteins (HSPs) and our previous demonstration of site-selective HSP-72 expression in cultured fibroblasts derived from extrathyroidal manifestations of Graves' disease (GD) prompted us to determine whether expression of the inducible 72- kilodalton HSP can be detected in human thyroid tissues. Immunohistochemistry was performed on formalin-fixed paraffin-embedded thyroid tissue specimens from patients with GD, Hashimoto's thyroiditis (HD), and multinodular goiter (MNG) as well as on normal thyroid tissue. A mouse monoclonal anti-HSP-72 antibody and an ultrasensitive avidin-biotin-peroxidase complex detection system were used for these studies. Striking differences in HSP-72 immunoreactivity were detected both between tissues from GD and HD compared with MNG and normal thyroid and between GD thyroid glands treated preoperatively with antithyroid medication and untreated GD glands. Strong HSP-72 reactivity in GD and HD tissues was detected in thyroid follicles as well lymphocytic infiltrates. No HSP-72 reactivity was detected in MNG or normal thyroid tissue. HSP-72 immunoreactivity was markedly reduced in GD glands that received preoperative antithyroid drug treatment. In conclusion, high levels of HSP-72 expression in autoimmune thyroid disease may reflect a state of chronic cellular stress, but could also represent an immunomodulatory factor of relevance in the autoimmune process in GD.

UR - http://www.scopus.com/inward/record.url?scp=0026588740&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026588740&partnerID=8YFLogxK

U2 - 10.1210/jc.74.4.724

DO - 10.1210/jc.74.4.724

M3 - Article

VL - 74

SP - 724

EP - 731

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 4

ER -