Immunogenicity of P/Q-type calcium channel in small cell lung cancer: Investigation of α1 subunit polyglutamine expansion

J. L. Black, T. R. Nelson, K. Snow, Vanda A Lennon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The ectopic expression of neuronal P/Q-type voltage-gated calcium channels in small cell lung carcinoma (SCLC) is thought to induce antisynaptic autoimmunity in the paraneoplastic Lambert-Eaton myasthenic syndrome. The gene CACNL1A4, encoding the principal (α1A) subunit of this calcium channel, is mutated in several inherited neurological disorders. One of these disorders (spinocerebellar ataxia, type 6, or SCA-6) involves the expansion of a trinucleotide (CAG) repeat unit. We hypothesized that a somatic CAG repeat instability of this gene in neoplastic cells might generate a non-self epitope capable of initiating autoimmunity to P/Q-type calcium channels. We therefore analyzed the CACNL1A4 gene in SCLC lines established from metastases derived from seven individual patients (four associated with Lambert-Eaton myasthenic syndrome, one associated with myasthenia gravis, and two not associated with neurological autoimmunity). We compared their CAG repeat numbers (determined by polymerase chain reaction (PCR) amplification followed by separation of products on a 6% polyacrylamide/8M urea gel) to published norms and to DNA from a patient with SCA-6. The number of CAG repeats in SCLC DNA fell within a normal range whether or not the neoplasm was complicated by neurological autoimmunity. Therefore, it is unlikely that somatically unstable CAG repeat units in the gene encoding the P/Q-type voltage-gated calcium channel account for this tumor protein's immunogenicity in the Lambert-Eaton myasthenic syndrome.

Original languageEnglish (US)
Pages (from-to)592-596
Number of pages5
JournalTissue Antigens
Volume54
Issue number6
DOIs
StatePublished - 1999

Fingerprint

Q-Type Calcium Channels
P-Type Calcium Channels
Small Cell Lung Carcinoma
Lambert-Eaton Myasthenic Syndrome
Autoimmunity
Calcium Channels
Genes
Spinocerebellar Ataxias
Trinucleotide Repeats
Myasthenia Gravis
DNA
Nervous System Diseases
Urea
Epitopes
Neoplasms
Reference Values
Gels
Neoplasm Metastasis
Polymerase Chain Reaction
polyglutamine

Keywords

  • Lambert-Eaton myasthenic syndrome
  • P/Q-type voltage-gated calcium channel
  • Paraneoplastic autoimmunity

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Immunogenicity of P/Q-type calcium channel in small cell lung cancer : Investigation of α1 subunit polyglutamine expansion. / Black, J. L.; Nelson, T. R.; Snow, K.; Lennon, Vanda A.

In: Tissue Antigens, Vol. 54, No. 6, 1999, p. 592-596.

Research output: Contribution to journalArticle

@article{1279b84e7678409496c6a4dfeb020284,
title = "Immunogenicity of P/Q-type calcium channel in small cell lung cancer: Investigation of α1 subunit polyglutamine expansion",
abstract = "The ectopic expression of neuronal P/Q-type voltage-gated calcium channels in small cell lung carcinoma (SCLC) is thought to induce antisynaptic autoimmunity in the paraneoplastic Lambert-Eaton myasthenic syndrome. The gene CACNL1A4, encoding the principal (α1A) subunit of this calcium channel, is mutated in several inherited neurological disorders. One of these disorders (spinocerebellar ataxia, type 6, or SCA-6) involves the expansion of a trinucleotide (CAG) repeat unit. We hypothesized that a somatic CAG repeat instability of this gene in neoplastic cells might generate a non-self epitope capable of initiating autoimmunity to P/Q-type calcium channels. We therefore analyzed the CACNL1A4 gene in SCLC lines established from metastases derived from seven individual patients (four associated with Lambert-Eaton myasthenic syndrome, one associated with myasthenia gravis, and two not associated with neurological autoimmunity). We compared their CAG repeat numbers (determined by polymerase chain reaction (PCR) amplification followed by separation of products on a 6{\%} polyacrylamide/8M urea gel) to published norms and to DNA from a patient with SCA-6. The number of CAG repeats in SCLC DNA fell within a normal range whether or not the neoplasm was complicated by neurological autoimmunity. Therefore, it is unlikely that somatically unstable CAG repeat units in the gene encoding the P/Q-type voltage-gated calcium channel account for this tumor protein's immunogenicity in the Lambert-Eaton myasthenic syndrome.",
keywords = "Lambert-Eaton myasthenic syndrome, P/Q-type voltage-gated calcium channel, Paraneoplastic autoimmunity",
author = "Black, {J. L.} and Nelson, {T. R.} and K. Snow and Lennon, {Vanda A}",
year = "1999",
doi = "10.1034/j.1399-0039.1999.540609.x",
language = "English (US)",
volume = "54",
pages = "592--596",
journal = "HLA",
issn = "2059-2302",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Immunogenicity of P/Q-type calcium channel in small cell lung cancer

T2 - Investigation of α1 subunit polyglutamine expansion

AU - Black, J. L.

AU - Nelson, T. R.

AU - Snow, K.

AU - Lennon, Vanda A

PY - 1999

Y1 - 1999

N2 - The ectopic expression of neuronal P/Q-type voltage-gated calcium channels in small cell lung carcinoma (SCLC) is thought to induce antisynaptic autoimmunity in the paraneoplastic Lambert-Eaton myasthenic syndrome. The gene CACNL1A4, encoding the principal (α1A) subunit of this calcium channel, is mutated in several inherited neurological disorders. One of these disorders (spinocerebellar ataxia, type 6, or SCA-6) involves the expansion of a trinucleotide (CAG) repeat unit. We hypothesized that a somatic CAG repeat instability of this gene in neoplastic cells might generate a non-self epitope capable of initiating autoimmunity to P/Q-type calcium channels. We therefore analyzed the CACNL1A4 gene in SCLC lines established from metastases derived from seven individual patients (four associated with Lambert-Eaton myasthenic syndrome, one associated with myasthenia gravis, and two not associated with neurological autoimmunity). We compared their CAG repeat numbers (determined by polymerase chain reaction (PCR) amplification followed by separation of products on a 6% polyacrylamide/8M urea gel) to published norms and to DNA from a patient with SCA-6. The number of CAG repeats in SCLC DNA fell within a normal range whether or not the neoplasm was complicated by neurological autoimmunity. Therefore, it is unlikely that somatically unstable CAG repeat units in the gene encoding the P/Q-type voltage-gated calcium channel account for this tumor protein's immunogenicity in the Lambert-Eaton myasthenic syndrome.

AB - The ectopic expression of neuronal P/Q-type voltage-gated calcium channels in small cell lung carcinoma (SCLC) is thought to induce antisynaptic autoimmunity in the paraneoplastic Lambert-Eaton myasthenic syndrome. The gene CACNL1A4, encoding the principal (α1A) subunit of this calcium channel, is mutated in several inherited neurological disorders. One of these disorders (spinocerebellar ataxia, type 6, or SCA-6) involves the expansion of a trinucleotide (CAG) repeat unit. We hypothesized that a somatic CAG repeat instability of this gene in neoplastic cells might generate a non-self epitope capable of initiating autoimmunity to P/Q-type calcium channels. We therefore analyzed the CACNL1A4 gene in SCLC lines established from metastases derived from seven individual patients (four associated with Lambert-Eaton myasthenic syndrome, one associated with myasthenia gravis, and two not associated with neurological autoimmunity). We compared their CAG repeat numbers (determined by polymerase chain reaction (PCR) amplification followed by separation of products on a 6% polyacrylamide/8M urea gel) to published norms and to DNA from a patient with SCA-6. The number of CAG repeats in SCLC DNA fell within a normal range whether or not the neoplasm was complicated by neurological autoimmunity. Therefore, it is unlikely that somatically unstable CAG repeat units in the gene encoding the P/Q-type voltage-gated calcium channel account for this tumor protein's immunogenicity in the Lambert-Eaton myasthenic syndrome.

KW - Lambert-Eaton myasthenic syndrome

KW - P/Q-type voltage-gated calcium channel

KW - Paraneoplastic autoimmunity

UR - http://www.scopus.com/inward/record.url?scp=0033398551&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033398551&partnerID=8YFLogxK

U2 - 10.1034/j.1399-0039.1999.540609.x

DO - 10.1034/j.1399-0039.1999.540609.x

M3 - Article

C2 - 10674974

AN - SCOPUS:0033398551

VL - 54

SP - 592

EP - 596

JO - HLA

JF - HLA

SN - 2059-2302

IS - 6

ER -