We describe a study testing fibrillar β-amyloid1-42 (Aβ42) vaccination in dogs. Three young beagles (4.6 years) were immunized twice with Aβ42 and a Th1 adjuvant (TiterMax Gold). Animals generated primarily IgG2 and IgM antibody responses, which were specific for the Aβ11-30 region of Aβ1-42. Next, 3 aged beagles (8.9-13.8 years) were immunized 4 times with Aβ42 and a Th2 adjuvant (Alum). We observed an acute increase in IgG2, a slower increase in IgG1 and Aβ antibodies of broader specificity (Aβ1-15> Aβ11-30> Aβ6-20). A nonsignificant increase in CSF Aβ1-40 and decrease in Aβ1-40/1-42 in cortex was detected. Canines may be a useful system for testing an Aβ vaccine.
- Alzheimer's disease
- Brain aging
ASJC Scopus subject areas
- Molecular Medicine
- Immunology and Microbiology(all)
- Public Health, Environmental and Occupational Health
- Infectious Diseases