Immune recognition of serum albumin-XIV. Cross-reactivity by T-lymphocyte proliferation of subdomains 3, 6 and 9 of bovine serum albumin

M. Zouhair Atassi, P. Michael Long, Kirk Beisel, Shigeki Sakata, Theodore Peters, Chella S. David

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Recently, we have shown that, with rabbit antibodies against BSA, the BSA fragments 115-184, 307-385 and 505-582 (essentially corresponding to subdomains 3, 6 and 9 of BSA) exhibit a cross-reaction which increases remarkably with the time antisera are obtained after the initial immunization. In the present report, we have examined whether this cross-reaction at the B-cell level takes place also at the T-cell level. Optimum conditions for T-cell proliferation to BSA and the three fragments were determined in terms of priming dose, challenging dose, time lymph nodes are obtained after immunization and finally duration of culture. Several strains of mice representing independent haplotypes and recombinant strains were examined for their responsiveness to BSA by T-lymphocyte proliferation, performed in the respective preimmune sera of the same mice. This afforded the identification of high-and low-responder strains to BSA. It was determined that the immune response to BSA is controlled by genes in the I-A subregion of the H-2 gene complex with some slight non-H-2 influences. In order to avoid the possibility of a genetic exclusion of response to a given antigenic site and to improve the chance of total site recognition, two high-responder strains (B10.M and B10.G) were crossed. Antibodies raised in (B10.M × B10.G)F1 mice recognized subdomains 3, 6 and 9 and these were found to be cross-reactive. Specific 125I-labelled antibodies isolated on a given subdomain-adsorbent were bound very well by adsorbents of the other two subdomains. T-cells from the F1 mice that had been primed with subdomain 3 responded to subdomain 3 and were also high responders to subdomain 6 and intermediate responders to subdomain 9. After priming with subdomain 6, the T-cells responded equally as well to subdomains 3 or 6 and slightly to subdomain 9. Finally, priming with subdomain 9, gave T-cells that responded to subdomain 9 and also gave high responses to subdomains 3 or 6. It was concluded that the cross-reactions originally observed at the B-cell level also take place at the T-cell level.

Original languageEnglish (US)
Pages (from-to)313-321
Number of pages9
JournalMolecular Immunology
Volume19
Issue number2
DOIs
StatePublished - 1982

Fingerprint

Bovine Serum Albumin
Serum Albumin
Immune Sera
T-Lymphocytes
Cross Reactions
Antibodies
Immunization
B-Lymphocytes
Haplotypes
Genes
Lymph Nodes
Cell Proliferation
Rabbits
Serum

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

Immune recognition of serum albumin-XIV. Cross-reactivity by T-lymphocyte proliferation of subdomains 3, 6 and 9 of bovine serum albumin. / Atassi, M. Zouhair; Long, P. Michael; Beisel, Kirk; Sakata, Shigeki; Peters, Theodore; David, Chella S.

In: Molecular Immunology, Vol. 19, No. 2, 1982, p. 313-321.

Research output: Contribution to journalArticle

Atassi, M. Zouhair ; Long, P. Michael ; Beisel, Kirk ; Sakata, Shigeki ; Peters, Theodore ; David, Chella S. / Immune recognition of serum albumin-XIV. Cross-reactivity by T-lymphocyte proliferation of subdomains 3, 6 and 9 of bovine serum albumin. In: Molecular Immunology. 1982 ; Vol. 19, No. 2. pp. 313-321.
@article{351a72e15eab46cf8d44e9a686d1a56b,
title = "Immune recognition of serum albumin-XIV. Cross-reactivity by T-lymphocyte proliferation of subdomains 3, 6 and 9 of bovine serum albumin",
abstract = "Recently, we have shown that, with rabbit antibodies against BSA, the BSA fragments 115-184, 307-385 and 505-582 (essentially corresponding to subdomains 3, 6 and 9 of BSA) exhibit a cross-reaction which increases remarkably with the time antisera are obtained after the initial immunization. In the present report, we have examined whether this cross-reaction at the B-cell level takes place also at the T-cell level. Optimum conditions for T-cell proliferation to BSA and the three fragments were determined in terms of priming dose, challenging dose, time lymph nodes are obtained after immunization and finally duration of culture. Several strains of mice representing independent haplotypes and recombinant strains were examined for their responsiveness to BSA by T-lymphocyte proliferation, performed in the respective preimmune sera of the same mice. This afforded the identification of high-and low-responder strains to BSA. It was determined that the immune response to BSA is controlled by genes in the I-A subregion of the H-2 gene complex with some slight non-H-2 influences. In order to avoid the possibility of a genetic exclusion of response to a given antigenic site and to improve the chance of total site recognition, two high-responder strains (B10.M and B10.G) were crossed. Antibodies raised in (B10.M × B10.G)F1 mice recognized subdomains 3, 6 and 9 and these were found to be cross-reactive. Specific 125I-labelled antibodies isolated on a given subdomain-adsorbent were bound very well by adsorbents of the other two subdomains. T-cells from the F1 mice that had been primed with subdomain 3 responded to subdomain 3 and were also high responders to subdomain 6 and intermediate responders to subdomain 9. After priming with subdomain 6, the T-cells responded equally as well to subdomains 3 or 6 and slightly to subdomain 9. Finally, priming with subdomain 9, gave T-cells that responded to subdomain 9 and also gave high responses to subdomains 3 or 6. It was concluded that the cross-reactions originally observed at the B-cell level also take place at the T-cell level.",
author = "Atassi, {M. Zouhair} and Long, {P. Michael} and Kirk Beisel and Shigeki Sakata and Theodore Peters and David, {Chella S.}",
year = "1982",
doi = "10.1016/0161-5890(82)90345-5",
language = "English (US)",
volume = "19",
pages = "313--321",
journal = "Molecular Immunology",
issn = "0161-5890",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - Immune recognition of serum albumin-XIV. Cross-reactivity by T-lymphocyte proliferation of subdomains 3, 6 and 9 of bovine serum albumin

AU - Atassi, M. Zouhair

AU - Long, P. Michael

AU - Beisel, Kirk

AU - Sakata, Shigeki

AU - Peters, Theodore

AU - David, Chella S.

PY - 1982

Y1 - 1982

N2 - Recently, we have shown that, with rabbit antibodies against BSA, the BSA fragments 115-184, 307-385 and 505-582 (essentially corresponding to subdomains 3, 6 and 9 of BSA) exhibit a cross-reaction which increases remarkably with the time antisera are obtained after the initial immunization. In the present report, we have examined whether this cross-reaction at the B-cell level takes place also at the T-cell level. Optimum conditions for T-cell proliferation to BSA and the three fragments were determined in terms of priming dose, challenging dose, time lymph nodes are obtained after immunization and finally duration of culture. Several strains of mice representing independent haplotypes and recombinant strains were examined for their responsiveness to BSA by T-lymphocyte proliferation, performed in the respective preimmune sera of the same mice. This afforded the identification of high-and low-responder strains to BSA. It was determined that the immune response to BSA is controlled by genes in the I-A subregion of the H-2 gene complex with some slight non-H-2 influences. In order to avoid the possibility of a genetic exclusion of response to a given antigenic site and to improve the chance of total site recognition, two high-responder strains (B10.M and B10.G) were crossed. Antibodies raised in (B10.M × B10.G)F1 mice recognized subdomains 3, 6 and 9 and these were found to be cross-reactive. Specific 125I-labelled antibodies isolated on a given subdomain-adsorbent were bound very well by adsorbents of the other two subdomains. T-cells from the F1 mice that had been primed with subdomain 3 responded to subdomain 3 and were also high responders to subdomain 6 and intermediate responders to subdomain 9. After priming with subdomain 6, the T-cells responded equally as well to subdomains 3 or 6 and slightly to subdomain 9. Finally, priming with subdomain 9, gave T-cells that responded to subdomain 9 and also gave high responses to subdomains 3 or 6. It was concluded that the cross-reactions originally observed at the B-cell level also take place at the T-cell level.

AB - Recently, we have shown that, with rabbit antibodies against BSA, the BSA fragments 115-184, 307-385 and 505-582 (essentially corresponding to subdomains 3, 6 and 9 of BSA) exhibit a cross-reaction which increases remarkably with the time antisera are obtained after the initial immunization. In the present report, we have examined whether this cross-reaction at the B-cell level takes place also at the T-cell level. Optimum conditions for T-cell proliferation to BSA and the three fragments were determined in terms of priming dose, challenging dose, time lymph nodes are obtained after immunization and finally duration of culture. Several strains of mice representing independent haplotypes and recombinant strains were examined for their responsiveness to BSA by T-lymphocyte proliferation, performed in the respective preimmune sera of the same mice. This afforded the identification of high-and low-responder strains to BSA. It was determined that the immune response to BSA is controlled by genes in the I-A subregion of the H-2 gene complex with some slight non-H-2 influences. In order to avoid the possibility of a genetic exclusion of response to a given antigenic site and to improve the chance of total site recognition, two high-responder strains (B10.M and B10.G) were crossed. Antibodies raised in (B10.M × B10.G)F1 mice recognized subdomains 3, 6 and 9 and these were found to be cross-reactive. Specific 125I-labelled antibodies isolated on a given subdomain-adsorbent were bound very well by adsorbents of the other two subdomains. T-cells from the F1 mice that had been primed with subdomain 3 responded to subdomain 3 and were also high responders to subdomain 6 and intermediate responders to subdomain 9. After priming with subdomain 6, the T-cells responded equally as well to subdomains 3 or 6 and slightly to subdomain 9. Finally, priming with subdomain 9, gave T-cells that responded to subdomain 9 and also gave high responses to subdomains 3 or 6. It was concluded that the cross-reactions originally observed at the B-cell level also take place at the T-cell level.

UR - http://www.scopus.com/inward/record.url?scp=0020052299&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020052299&partnerID=8YFLogxK

U2 - 10.1016/0161-5890(82)90345-5

DO - 10.1016/0161-5890(82)90345-5

M3 - Article

C2 - 6178957

AN - SCOPUS:0020052299

VL - 19

SP - 313

EP - 321

JO - Molecular Immunology

JF - Molecular Immunology

SN - 0161-5890

IS - 2

ER -