Immediate early gene X-1 interacts with proteins that modulate apoptosis

Rajiv Kumar, Ward Lutz, Elena Frank, Hee Jeong Im

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Immediate early gene X-1 (IEX-1) modulates apoptosis, cellular growth, mechanical strain-induced cardiac hypertrophy, and vascular intimal hyperplasia. To determine how IEX-1 alters apoptosis, we performed yeast two-hybrid studies using IEX-1 as the "bait" protein, and examined interactions between IEX-1 and proteins expressed by a human kidney cDNA expression library. We found that IEX-1 interacts with several proteins of which at least four are known to play a role in the regulation of apoptosis: (1) calcium-modulating cyclophilin ligand; (2) tumor necrosis factor-related apoptosis-inducing ligand (tumor necrosis factor superfamily, member 10); (3) ML-1 myeloid cell leukemia gene encoded protein; and (4) BAT3, a gene present in the major histo-compatibility complex. Our data suggest that IEX-1 may regulate apoptosis by directly interacting with various proteins involved in the control of apoptotic pathways.

Original languageEnglish (US)
Pages (from-to)1293-1298
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume323
Issue number4
DOIs
StatePublished - Oct 29 2004

Keywords

  • 1, 25-Dihydroxyvitamin D
  • Apoptosis
  • IEX-1
  • gly96

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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