Imaging luciferase-expressing viruses

Michael A. Barry, Shannon May, Eric A. Weaver

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

Optical imaging of luciferage gene expression has become a powerful tool to track cells and viruses in vivo in small animal models. Luciferase imaging has been used to study the location of infection by replication-defective and replication-competent viruses and to track changes in the distribution of viruses in mouse models. This approach has also been used in oncolytic studies as a noninvasive means to monitor the growth and killing of tumor cells modified with luciferase genes. In this chapter, we describe the techniques used for luciferase imaging as have been applied to track replication-defective and replication-competent adenoviruses in mouse and hamster models of oncolysis and virus pharmacology. Although these methods are simple, the process of obtaining accurate luciferase imaging data has many caveats that are discussed.

Original languageEnglish (US)
Title of host publicationOncolytic Viruses
Subtitle of host publicationMethods and Protocols
EditorsDavid Kirn, David Kirn, Steve Thorne, Ta-Chiang Liu, Ta-Chiang Liu
Pages79-87
Number of pages9
DOIs
StatePublished - Jan 1 2012

Publication series

NameMethods in Molecular Biology
Volume797
ISSN (Print)1064-3745

    Fingerprint

Keywords

  • Adenovirus
  • In vivo imaging
  • Luciferase
  • Luciferin
  • Luminescence

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Barry, M. A., May, S., & Weaver, E. A. (2012). Imaging luciferase-expressing viruses. In D. Kirn, D. Kirn, S. Thorne, T-C. Liu, & T-C. Liu (Eds.), Oncolytic Viruses: Methods and Protocols (pp. 79-87). (Methods in Molecular Biology; Vol. 797). https://doi.org/10.1007/978-1-61779-340-0_6