Imageable fluorescent metastasis resulting in transgenic GFP mice orthotopically implanted with human-patient primary pancreatic cancer specimens

Atsushi Suetsugu, Matthew Katz, Jason Fleming, Mark Truty, Ryan Thomas, Shigetoyo Saji, Hisataka Moriwaki, Michael Bouvet, Robert M. Hoffman

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Tumors from pancreatic cancer patients were established in NODISClD mice immediately after surgery and subsequently passaged orthotopically in transgenic nude mice ubiquitously expressing green fluorescent protein (GFP). The primary patient tumors acquired GFP-expressing stroma. Subsequent liver metastases, and disseminated peritoneal metastases maintained the stroma from the primary tumor, and possibly recruited additional GFP-expressing stroma, resulting in their very bright fluorescence. The GFP-expressing stroma included cancer-associated fibroblasts and tumor-associated macrophages in both the primary and metastatic tumors. This imageable model of metastasis from a patient-tumor is an important advance over patient "tumorgraft" models currently in use, which are implanted subcutaneously, do not metastasize and are not imageable. The new imageable model of patient pancreatic cancer metastasis provides unique opportunities to identify current and novel antimetastatic therapeutics for individual patients.

Original languageEnglish (US)
Pages (from-to)1175-1180
Number of pages6
JournalAnticancer research
Volume32
Issue number4
StatePublished - Apr 2012

Keywords

  • GFP
  • Human-patient pancreatic cancer
  • Imaging
  • Metastasis
  • Microenvironment
  • Nude mice
  • Stroma
  • Tumor

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Suetsugu, A., Katz, M., Fleming, J., Truty, M., Thomas, R., Saji, S., Moriwaki, H., Bouvet, M., & Hoffman, R. M. (2012). Imageable fluorescent metastasis resulting in transgenic GFP mice orthotopically implanted with human-patient primary pancreatic cancer specimens. Anticancer research, 32(4), 1175-1180.